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A method for rapidly degrading high-molecular-weight polyamino acids and its application

A high-molecular-weight, polyamino acid technology, applied in the fields of macromolecule and microwave chemistry, and bioengineering, can solve the problems of high molecular weight polyamino acids, achieve high application value, high thermal efficiency, and improve reproducibility

Inactive Publication Date: 2015-10-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method that is faster, more efficient, energy-consuming and cost-effective than ordinary acid hydrolysis and enzymatic hydrolysis, according to the problem that the molecular weight of natural collagen, protein and existing industrially produced polyamino acids is too high. low degradation method

Method used

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  • A method for rapidly degrading high-molecular-weight polyamino acids and its application
  • A method for rapidly degrading high-molecular-weight polyamino acids and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Weigh 1g of γ-polyglutamic acid and dissolve it in 50mL of pure water, and adjust the pH value to 0.0 with hydrochloric acid respectively. A three-necked round-bottomed flask is used as a container, one of which is the feeding port, one port is connected to the infrared temperature detection head, and the other port is connected to the reflux condensing device. The power of the microwave reactor was set at 500 W, the temperature of the microwave reaction was set at 70°C, and the reactions were performed for 2, 4, 8, 16, 30, 60, 90, and 120 minutes, respectively. The resulting degradation product was dialyzed in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 7000 Da, and then freeze-dried to obtain a powder product.

Embodiment 2

[0045] Weigh 1g of γ-polyglutamic acid and dissolve it in 50mL of pure water, and adjust the pH to 1.0 with hydrochloric acid. A three-necked round-bottomed flask is used as a container, one of which is the feeding port, one port is connected to the infrared temperature detection head, and the other port is connected to the reflux condensing device. The power of the microwave reactor was set at 500 W, the temperature of the microwave reaction was set at 70°C, and the reactions were performed for 2, 4, 8, 16, 30, 60, 90, and 120 minutes, respectively. The resulting degradation product was dialyzed in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 7000 Da, and then freeze-dried to obtain a powder product.

Embodiment 3

[0047] Weigh 1g of γ-polyglutamic acid and dissolve it in 50mL of pure water, and adjust the pH value to 2.0 with hydrochloric acid respectively. A three-necked round-bottomed flask is used as a container, one of which is the feeding port, one port is connected to the infrared temperature detection head, and the other port is connected to the reflux condensing device. The power of the microwave reactor was set at 500 W, the temperature of the microwave reaction was set at 70°C, and the reactions were performed for 2, 4, 8, 16, 30, 60, 90, and 120 minutes, respectively. The resulting degradation product was dialyzed in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 7000 Da, and then freeze-dried to obtain a powder product.

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Abstract

The invention discloses a method for rapidly degrading high-molecular-weight polyamino acid, collagen and protein and an application of the method. According to the method, under the condition of microwave heating, due to the influence of a thermal effect and a non-thermal effect of microwaves, the degradation velocity of the high-molecular-weight polyamino acid, collagen and protein is greatly improved and can reach more than 2 times that of a conventional hydrothermal system, and the degradation velocity is improved to be more than 90 times that of the conventional hydrothermal system by adjusting and controlling the temperature and pH value of the system. A microwave-assisted degradation technology adopted by the method has significant characteristics of low energy consumption, high efficiency and capability of easily realizing continuous large-scale production, so that the application fields of the high-molecular-weight polyamino acid, collagen and protein can be further expanded and the application values of the high-molecular-weight polyamino acid, collagen and protein can be improved hopefully.

Description

technical field [0001] The invention relates to the fields of bioengineering, macromolecules and microwave chemistry, in particular to a method and application for rapidly degrading high-molecular-weight polyamino acids. Background technique [0002] Polyamino acid refers to a class of polymers synthesized by chemical or microbial methods from the same amino acid monomer, such as ε-polylysine, γ-polyglutamic acid, polyaspartic acid, polyornithine, etc. . Polyamino acids have many superior characteristics: (1) As a biomacromolecule, polyamino acids have good biocompatibility and tissue affinity, which can promote tissue repair and cell growth; Under the action, it can be degraded into amino acids, which are easily absorbed and metabolized by the body, and are safe and non-toxic; (3) Polyamino acids are multivalent ions, which can replace non-pathogenic viruses as the carrier of gene therapy. (4) Polyamino acid has good resistance to organic solvent corrosion, heat resistanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/48
Inventor 李皓张梁张超康华蒋庆
Owner SUN YAT SEN UNIV
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