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Preparation method and application of double-mode molecular probe based on gadolinium doped carbon point

A molecular probe and dual-mode technology, applied in the field of nanomaterials, can solve the problems of limitation, sensitivity, selectivity, resolution and insufficient safety, etc., and achieve the effects of high yield, environmental friendliness and simple process

Inactive Publication Date: 2013-09-04
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Description
  • Claims
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Problems solved by technology

[0006] It can be seen from the above that the existing various imaging technologies have their own advantages, but they also have shortcomings in sensitivity, selectivity, resolution, and safety. Therefore, they are limited in experimental research or clinical application.

Method used

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  • Preparation method and application of double-mode molecular probe based on gadolinium doped carbon point
  • Preparation method and application of double-mode molecular probe based on gadolinium doped carbon point
  • Preparation method and application of double-mode molecular probe based on gadolinium doped carbon point

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preparation example Construction

[0035] The method for preparing gadolinium-doped carbon dots of the present invention comprises the following steps:

[0036] A) Use carbohydrate aqueous solution as carbon source, mix with polyol;

[0037] B) with mineral acid as catalyst, add the system described in step A;

[0038] C) the system described in step B, adds gadolinium ion;

[0039] D) with microwave as heat source, the system in step C is heated;

[0040] E) Dialysis and purification with a dialysis bag with a molecular weight cut-off of 100 Da or 500 Da to obtain an aqueous solution of gadolinium-doped carbon dots.

[0041] Another object of the present invention is to use the prepared gadolinium-doped carbon dots for optical imaging, which comprises the following steps:

[0042] A) The gadolinium-doped carbon dots were incubated with cells at 37°C, 5% CO 2 co-incubated under conditions;

[0043] B) Laser confocal microscope to observe the optical imaging of cells by the gadolinium-doped carbon dots in A...

Embodiment 1

[0049] 1) take 1ml mass concentration as 30% sucrose aqueous solution as carbon source, mix with diethylene glycol (or glycerol) in a volume ratio of 1:3, and the total reaction volume is 4ml;

[0050] 2) using 200 μl of concentrated sulfuric acid (or concentrated hydrochloric acid) as a catalyst, adding the system in step 1;

[0051] 3) Add 100mg of GdCl 3 , add the described system in step 2;

[0052] 4) using the household microwave oven of 500-1000W as a heat source, microwave heating the system of step 3 for 50s;

[0053] 5) Dialyzing and purifying with a dialysis bag with a molecular weight cut-off of 100 Da to obtain an aqueous solution of carbon dots;

[0054]6) Characterization of photoluminescence properties of the obtained carbon dots: no color is displayed under sunlight ( figure 1 A), the carbon quantum dot aqueous solution sample was irradiated with an ultraviolet lamp with a wavelength of 365 nm, and a clear green light could be observed ( figure 1 B); Obtai...

Embodiment 2

[0056] 1) Seed C6 cells in a 6-well culture dish with a coverslip at 37°C, 5% CO 2 cultivated in an incubator;

[0057] 2) After the cells adhere to the wall, aspirate the supernatant, add the medium containing the gadolinium-doped carbon dots prepared by the present invention, at 37° C., 5% CO 2 Incubate for 24h in the incubator;

[0058] 3) Take out the coverslip, wash with PBS buffer, and fix the cells;

[0059] 4) Observe the cell imaging under a laser confocal microscope (405 nm) ( image 3 ), gadolinium-doped carbon dot-labeled cells emit green fluorescence under excitation at 405 nm. in image 3 A is the bright field laser confocal image of C6 tumor cells, image 3 B is a laser confocal image under excitation at 405 nm.

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Abstract

The invention discloses a double-mode molecular probe based on a gadolinium doped carbon point. The double-mode molecular probe based on the gadolinium doped carbon point is obtained by the following method: (A) adding carbohydrate which is used as a carbon source to a polyhydric alcohol solvent to prepare a mixed solution; (B) adding inorganic acid which is used as a catalyst into the mixed solution obtained in the step A; (3) adding GdCl3 which is used as a gadolinium source into the mixed solution obtained in the step B; (D) carrying out microwave heating on the product obtained in the step C for 30-60 seconds; and (E) dialyzing the heated product by using a dialysis bag with molecular weight cutoff of 100Da or 500Da to obtain an aqueous solution of the gadolinium doped carbon point. The gadolinium doped carbon point can be directly applied to MRI (Magnetic Resonance Imaging)-optical double-mode imaging of a tumor cell.

Description

technical field [0001] The invention belongs to the field of nanomaterials, and in particular relates to a gadolinium-doped carbon dot as an MRI-optical dual-mode molecular probe. [0002] The present invention also relates to the "bottom-up" preparation method of the above-mentioned gadolinium-doped carbon dot-based dual-mode molecular probe. [0003] The present invention also relates to the application of the above-mentioned gadolinium-doped carbon dot-based dual-mode molecular probe in cell labeling. [0004] The present invention also relates to the above-mentioned gadolinium-doped carbon dot-based dual-mode molecular probe as an MRI T1 contrast enhancer. Background technique [0005] Molecular imaging technology enables real-time, dynamic and non-invasive imaging of physiological and pathological changes in biological organisms at the molecular level. Compared with traditional medical imaging technology, molecular imaging technology performs in vivo imaging of change...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/77G01N21/64A61K49/06
Inventor 顾微宫宁强王昊肖宁陈璐李彦楠叶玲
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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