Closed nucleic acid chromatographic test paper detection kit preserved at normal temperature and detection method

Abstract

The invention discloses a method for preserving nucleic acid amplification reagents at normal temperature. Partial nucleic acid amplification reagents or all reagents are converted to solid state through a solidifying process and preserved stably at normal temperature. The invention further discloses a closed nucleic acid chromatographic test paper detection kit preserved at normal temperature. The kit comprises a nucleic acid extraction reagent of a sampler, the nucleic acid amplification reagent preserved at normal temperature, a closed chromatographic test paper detection device and an innocent treatment method of nucleic acid amplification products. The closed chromatographic test paper detection device comprises immunochromatographic test paper and a closed detection device, wherein the immunochromatographic test paper is fixedly arranged in the closed detection device, and sample liquor detected cannot be contacted with external environment. In addition, the invention further discloses a method for nucleic acid detection by using the kit. According to the invention, the nucleic acid reagents can be conveyed and preserved at normal temperature and extracted simply and quickly, and the nucleic acid amplification products are hermetically, quickly and sensitively detected, so that cross infection is avoided.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a method for storing nucleic acid amplification reagents at normal temperature; in addition, the invention also relates to a closed nucleic acid chromatography test paper detection kit and a detection method thereof for storage at normal temperature, and also discloses nucleic acid amplification The harmless disposal method of the product. Background technique [0002] Since 1985, the American Cetus company used Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) to realize the specific amplification of nucleic acid, this technology has been applied to medical in vitro diagnosis and medical treatment because of its high sensitivity, high specificity and high efficiency. Food safety testing and other fields. In recent years, with the rapid development of molecular biology and the extensive application of biophysical techniques, new nucleic acid amplification modes are also emerging. ...

AI Technical Summary

Problems solved by technology

However, these methods and measures require strict experimental conditions and expensive experimental facilities and equipment, and are not suitable for popularization to grassroots users.

The second is to use dUTP instead of dTTP in the nucleic acid amplification reaction, and add UNG enzyme to degrade the uracil base in the contaminated product U-DNA, and completely hydrolyze and break the U-DNA chain under high temperature denaturing conditions to eliminate Due to the amplification caused by the contaminated product, the specificity of the amplification result is guaranteed, but this method requires variable temperature operation, many steps, high cost, and there is a certain probability of contamination, which limits its application

Gu Jiayong et al. (CN1888902) invented a closed nucleic acid lateral flow chromatography detection device, which can improve detection sensitivity and initially solve the problem of amplification product contamination

However, the disadvantages of this device are: 1. The device is large in size and is not suitable for large-scale production and transportation of finished products; 2. The test strips in the device are complicated to install and are not suitable for large-scale test paper assembly and production; 3. After the device is restarted and used , can be reopened easily and without traces, so the nucleic acid amplification product is only relatively closed. If the device that has been reacted is opened unintentionally, the contamination of the amplification product is inevitable; 4. The device In this method, only a single reaction solution can be detected, but multiple reaction solutions cannot be detected, that is, the detection of multiple samples cannot be completed on one test strip in the form of multiple reaction solutions

This is not conducive to environmental protection, but also increases the difficulty and cost of injection molding production

Images