Bacterial cellulose facial mask with gradient structure and preparation method of facial mask
A technology of bacterial cellulose and gradient structure, applied in microorganism-based methods, biochemical equipment and methods, preparations for skin care, etc., can solve the problems of uneven film, single film structure, etc. , the effect of good experience
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Embodiment 1
[0049] 1) the deployment of fermentation broth;
[0050] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, methyl Cellulose 0.1, wheat starch 0.1;
[0051] The pH of the culture solution is 4.0;
[0052] Heat the culture medium to 50°C to dissolve methyl cellulose and evenly disperse it in the culture medium, at the same time make the wheat starch gelatinize and evenly disperse in the fermentation medium, sterilize with high pressure steam, irradiate with ultraviolet light and cool to room temperature , through pure oxygen, the fermentation medium is obtained;
[0053] 2) strain expansion;
[0054] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 5 pieces / ml.
[0055] 3) Static cultivation;
[0056] Transfer the ...
Embodiment 2
[0060] 1) the deployment of fermentation broth;
[0061] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 3, peptone 0.2, yeast extract 0.2, citric acid 0.05, disodium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.05, methyl Cellulose 0.5, wheat starch 0.5;
[0062] The pH of the culture solution is 5.0;
[0063] Heat the culture medium to 70°C to dissolve the methylcellulose and disperse it uniformly in the culture medium, meanwhile, gelatinize the wheat starch and disperse it evenly in the fermentation medium, sterilize it with high pressure steam, irradiate it with ultraviolet light and cool it down to room temperature , through pure oxygen, the fermentation medium is obtained;
[0064] 2) strain expansion;
[0065] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 6 pieces / ml.
[0066] 3) Static cultivation;
[0067] Tran...
Embodiment 3
[0071] 1) the deployment of fermentation broth;
[0072] Components of the culture medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 5, peptone 0.5, yeast extract 0.5, citric acid 0.1, disodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1, methyl Cellulose 1, Wheat Starch 1;
[0073] The pH of the culture solution is 6.0;
[0074] Heat the culture medium to 80°C to dissolve the thickener and evenly disperse it in the culture medium, and at the same time make the wheat starch gelatinize and evenly disperse in the fermentation medium, sterilize it with high pressure steam, irradiate it with ultraviolet light and cool it to room temperature. Through pure oxygen, the fermentation medium is obtained;
[0075] 2) strain expansion;
[0076] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of cells of Acetobacter xylinum is 2×10 7 pieces / ml.
[0077] 3) Static cultivation;
[0078] Transfer the ex...
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