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Method for preparing spirulina polypeptide-chitosan nano particles

A technology of chitosan nanoparticles and spirulina polypeptides, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve problems such as human injury

Inactive Publication Date: 2013-05-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current treatment methods for cancer patients are mainly chemotherapy and radiotherapy, which seriously damage the human body.

Method used

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  • Method for preparing spirulina polypeptide-chitosan nano particles
  • Method for preparing spirulina polypeptide-chitosan nano particles
  • Method for preparing spirulina polypeptide-chitosan nano particles

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The preparation method of spirulina polypeptide-chitosan nanoparticle, the steps are as follows:

[0033] (1) Dissolve 20 g of spirulina powder in 300 mL of Milli-Q water, and stir magnetically for 30 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was disrupted by ultrasonication for 300 times at 100 W ultrasonic power at intervals of 10 s for 5 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at 4 °C and 8000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then rapidly freeze-dried in vacuum to obtain the Spirulina protein powder.

[0034] (2) Based on the spirulina protein powder obtained in step (1), configure ...

Embodiment 2

[0042] The preparation method of spirulina polypeptide-chitosan nanoparticle, the steps are as follows:

[0043] (1) Dissolve 10 g of spirulina powder in 200 mL of Milli-Q water, and stir magnetically for 20 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was broken by sonicating 100 times at an ultrasonic power of 50 W at intervals of 5 s and 4 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at 4 °C and 6000 r / min for 25 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the Spirulina protein powder.

[0044] (2) Based on the spirulina protein powder obtained in step (1), configure a 2% spi...

Embodiment 3

[0048] The preparation method of spirulina polypeptide-chitosan nanoparticle, the steps are as follows:

[0049] (1) 30 g of spirulina powder was dissolved in 500 mL of Milli-Q water, and magnetically stirred for 40 min at room temperature. After it was completely dissolved, it was frozen in a -20 °C refrigerator for 80 min, and then thawed in a 4 °C refrigerator, and the freezing and thawing was repeated three times. Then, the Spirulina wall was broken by sonicating 200 times at 150 W ultrasonic power at intervals of 10 s for 6 s, so that the protein was freed. To prevent protein denaturation from the heat generated during sonication, samples were placed in an ice bath. Finally, the obtained solution was centrifuged at a temperature of 4 °C and a speed of 5000 r / min for 40 min to remove the precipitate to obtain a protein supernatant, and then rapidly freeze-dried in a vacuum to obtain Spirulina protein powder.

[0050] (2) Based on the spirulina protein powder obtained in ...

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Abstract

The invention provides a method for preparing spirulina polypeptide-chitosan nano particles. The method mainly comprises the following steps: extracting spirulina proteins by employing a method of combining multigelation and ultrasonication, hydrolyzing the spirulina proteins through trypsin, separating and purifying by a sephadex column, filtering through an ultrafiltration centrifugation tube, and preparing the spirulina polypeptide-chitosan nano particles by employing a sodium tripolyphosphate ion exchange method. The obtained spirulina polypeptide-chitosan nano particles Y2-CS have the following antitumor activities that the spirulina polypeptide-chitosan nano particles Y2-CS have a certain function of inhibiting in-vitro growth of human breast cancer cell MCF-7 and have a certain dose relationship; and when the concentration is 250mug / mL, the highest inhibition rate can be 26.05 percent. Therefore, the spirulina polypeptide-chitosan nano particles contribute to developing and utilizing antitumor health-care foods and medical products.

Description

technical field [0001] The invention relates to the field of spirulina deep processing and biotechnology, in particular to a preparation method of spirulina polypeptide-chitosan nanoparticles. Background technique [0002] Spirulina (Spirulina platensis) is a kind of cyanobacteria, which has physiological functions such as liver protection, anti-oxidation, anti-virus, anti-cancer and enhancing immunity. One of the highest species. The protein composition of spirulina is closest to the standard stipulated by the World Food and Agriculture Organization (FAO). It contains 18 kinds of amino acids, and has all 8 kinds of amino acids necessary for the human body. It is a high-quality protein source. Available in dry powder and tablet form. Due to its limited solubility, which is not conducive to direct absorption by the human body, its application is limited to a certain extent. Therefore, the separation and purification of active peptides from Spirulina has huge social and econ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K38/02A61K47/36C12P21/06A61P35/00
Inventor 张学武张博超
Owner SOUTH CHINA UNIV OF TECH
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