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Application of terpene compound

A technology of terpenoids and drugs, applied in the field of natural medicinal chemistry, can solve the problems of unclear antifungal active ingredients and achieve the effect of preventing and/or treating fungal infections

Inactive Publication Date: 2013-05-08
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its antifungal active ingredient is not clear

Method used

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  • Application of terpene compound
  • Application of terpene compound
  • Application of terpene compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Isolation and preparation of formula I compound

[0023] Dried Kochia scoparia medicinal material, pulverized, subjected to CO 2 Supercritical extraction to remove grease, the dregs were extracted three times with 80% ethanol reflux, the extract was concentrated, the obtained extract was mixed with diatomaceous earth (1:1), and petroleum ether, chloroform, ethyl acetate, methanol were eluted in sequence, and the obtained The methanol eluted part was then extracted with water and saturated n-butanol to obtain the n-butanol part. The n-butanol fraction was prepared through repeated silica gel column chromatography, gel Sephadex LH-20 chromatography, and medium-low pressure reversed-phase silica gel liquid phase to obtain the compound of formula I.

[0024] Formula I compound structure is by ESI-MS, 1 H-NMR and 13 C-NMR identification. ESI-MS measuring instrument: MSD-Trap-XCT, Q-Tof micro (ESI-MS) type mass spectrometer (Agilent Company), 1 H-NMR measur...

Embodiment 2

[0029] Embodiment 2: the antifungal activity determination of formula I compound

[0030] The compound obtained in Example 1 was tested for its antibacterial activity against Candida albicans by the paper sensitive sheet method.

[0031] Preparation of the test solution: take the compound of formula 1 and dissolve it in methanol to prepare 5mg·mL -1 solution, set aside.

[0032] Preparation of bacterial suspension: Take an appropriate amount of 0.85% sterile saline and add it to the activated Candida albicans slant test tube, wash the surface bacteria with an inoculation spatula, and pour it into a sterile small triangular flask with glass beads , shattered by shaking, and then diluted the bacterial solution with normal saline to a bacterial concentration of 10 9 / ml, stored in a refrigerator at 4°C until use.

[0033] Take 1.5ml of Candida albicans solution and place it in 100ml of yeast extract peptone glucose agar medium cooled to 48°C-50°C after melting, shake it up qui...

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Abstract

The present invention provides an application of a terpene compound represented by a formula I in preparation of drugs for prevention and / or treatment of fungal infections. The compound represented by the formula I can be adopted as a novel antifungal active ingredient with characteristics of high efficiency and low toxicity so as to provide a good fungi inhibition effect, wherein drugs made from the compound further have characteristics of high efficiency and low toxicity, such that prevention and / or treatment of fungal infections, especially deep fungal infections can be achieved.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to the application of a terpenoid compound. Background technique [0002] Mycoses can be divided into superficial fungal infections that occur in the skin, mucous membranes, and subcutaneous tissues, and deep fungal infections that invade deep layers of the skin and visceral tissues (also known as invasive fungal infections). Early mycosis is mostly superficial fungal infection, and deep fungal infection is rare. In recent decades, with the extensive use of broad-spectrum antibiotics, hormones, immunosuppressants and anti-tumor drugs, the popularization of catheter intervention, organ transplantation and other treatment technologies, and the continuous growth of serious diseases such as AIDS, deep fungal infections have increased significantly. According to the analysis of my country's nosocomial infection monitoring network, the fungal infection rate in hospitals rose fro...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P31/10
Inventor 华茉莉徐云辉张念黄浩
Owner SHANGHAI INST OF PHARMA IND
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