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Freezing and drying protective agent used for infectious bronchitis virus HI antigen and application of freezing and drying protective agent in preparation of HI antigen

A technology for freeze-drying protective agent and bronchitis, which is applied in the directions of antiviral agents, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. To achieve the effect of improving heat resistance

Active Publication Date: 2013-04-24
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the test results of the prepared M41 freeze-dried HI antigen are very consistent with the test results of imported antigens, the guarantee period is only about 1 year. How to extend the preservation period urgently needs the innovation of researchers.

Method used

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  • Freezing and drying protective agent used for infectious bronchitis virus HI antigen and application of freezing and drying protective agent in preparation of HI antigen
  • Freezing and drying protective agent used for infectious bronchitis virus HI antigen and application of freezing and drying protective agent in preparation of HI antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of lyoprotectant (volume 1L)

[0025] Preparation of liquid A: Weigh 50g of trehalose and 20g of gelatin, dissolve in a small amount of deionized water, then dilute to 1L with deionized water, and autoclave at 116°C for 30 minutes;

[0026] Liquid B: Weigh 1 g of sodium azide and 10 g of sodium thiosulfate, dissolve them in a small amount of deionized water, then dilute to 1 L with deionized water, and sterilize with a 0.22 μm membrane filter.

[0027] Prepare the protective agent used in the freeze-drying technology of chicken infectious bronchitis virus HI antigen by mixing equal volumes of liquid A and liquid B.

Embodiment 2

[0028] Example 2 Preparation of lyoprotectant (volume 1L)

[0029] Preparation of liquid A: Weigh 50g of trehalose and 60g of gelatin, dissolve in a small amount of deionized water, then dilute to 1L with deionized water, and autoclave at 116°C for 30 minutes;

[0030] Liquid B: Weigh 5 g of sodium azide and 8 g of sodium thiosulfate, dissolve them in a small amount of deionized water, then dilute to 1 L with deionized water, and sterilize with a 0.22 μm filter membrane.

[0031] Prepare the protective agent used in the freeze-drying technology of chicken infectious bronchitis virus HI antigen by mixing equal volumes of liquid A and liquid B.

Embodiment 3

[0032] Example 3 Preparation of lyoprotectant (volume 1L)

[0033] Preparation of liquid A: Weigh 20g of trehalose and 80g of gelatin, dissolve in a small amount of deionized water, then dilute to 1L with deionized water, and autoclave at 116°C for 30 minutes;

[0034] Liquid B: Weigh 2 g of sodium azide and 8 g of sodium thiosulfate, dissolve them in a small amount of deionized water, then dilute to 1 L with deionized water, and sterilize with a 0.22 μm membrane filter.

[0035] Prepare the protective agent used in the freeze-drying technology of chicken infectious bronchitis virus HI antigen by mixing equal volumes of liquid A and liquid B.

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PUM

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Abstract

The invention relates to a freezing and drying protective agent used for an infectious bronchitis virus HI antigen and application of the freezing and drying protective agent in preparation of the HI antigen. The protective agent is formed by mixing a solution A and a solution B having the same volume, wherein according to the quantity volume percentage, the solution A contains 1%-10% of mycose and 2%-10% of gelatin; and according to the quantity volume percentage, the solution B contains 0.1%-1% of sodium azide and 0.5%-1% of sodium thiosulfate. In comparison with the HI antigen prepared by adopting a regular method, the storage life of the infectious bronchitis virus HI antigen prepared by adopting the freezing and drying protective agent disclosed by the invention is prolonged from one year to three years, the heat resistance of the HI antigen is improved, and wide application foreground is obtained.

Description

technical field [0001] The invention relates to a method for preparing infectious bronchitis virus HI antigen, in particular to a freeze-drying protectant for chicken infectious bronchitis virus HI antigen and its application in preparing the HI antigen. Belongs to the field of biotechnology. Background technique [0002] Chicken infectious bronchitis (Infectious Bronchitis, referred to as IB) is an acute highly contagious disease caused by chicken infectious bronchitis virus (Infectious Bronchitis Virus, referred to as IBV). Show as symptoms such as coughing, sneezing, trachea and rales. The egg production of laying hens decreased, and deformed eggs were produced, and the performance of young chickens was particularly serious. Certain strains cause kidney and intestinal disease. Chicken infectious bronchitis has caused serious economic losses to the world poultry industry. Due to the high variability of IBV, there are many serotypes, and the cross-protective reaction be...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K47/42A61K47/36A61K47/02A61P31/14
Inventor 孙德君张杨丁国杰刘鑫莹武佳斌孟福强胡瑞鸿李敏张明艳丛秋实张婷婷
Owner 哈药集团生物疫苗有限公司
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