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Avidin-biotin system cell patterning-based chip and preparation and applications thereof

A cell patterning and biotin technology, which is applied in the field of biological microsystems, can solve the problems of many animal experiments, large amount of drugs, complex technology, etc., and achieve the effect of low requirements for experimental conditions, simple method, and firm combination

Active Publication Date: 2013-04-03
TSINGHUA UNIV
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Problems solved by technology

[0002] The traditional drug screening method determines the key biological substance that causes the disease by tracking the biochemical pathway of animal tissue pathology, and then uses the target molecule of this substance for drug screening, which has the advantages of complex technology, low throughput, many animal experiments, large drug consumption, and high cost. shortcoming

Method used

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  • Avidin-biotin system cell patterning-based chip and preparation and applications thereof
  • Avidin-biotin system cell patterning-based chip and preparation and applications thereof
  • Avidin-biotin system cell patterning-based chip and preparation and applications thereof

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Embodiment Construction

[0018] Further description will be made below in conjunction with the accompanying drawings and specific embodiments.

[0019] The cell array structure after separation and positioning, such as figure 1 shown. The invention uses photolithography and CVD to pattern HMDS and PEG on a glass substrate, and then uses Biotin-BSA, avidin, and a sandwich structure of cells to form a (single) cell array on the HMDS array.

[0020] 1. Graphical treatment of HMDS and PEG passivation area:

[0021] Ultrasonic cleaning of glass slides with Piranha lotion to remove metal ions and organic matter on the surface of glass slides; spin-coat photoresist on the cleaned glass slides, and pre-baking; photoresist exposure and intermediate baking ; develop in NaOH developing solution to obtain photoresist patterns and harden the film; use chemical vapor deposition, cool in HMDS vapor, deposit HMDS on the glass slide without photoresist; use acetone to remove the glue, remove all photoresist, Form a...

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Abstract

The invention discloses an avidin-biotin system cell patterning-based chip and preparation and applications thereof, belonging to the technical field of a biological micro system. The method comprises the steps: preparing a bioaffinity hexamethyl disilazane (HMDS) lattice array and bio-passivated polyethylene glycol pattering area by adopting photoetching and chemical precipitation methods; then forming a sandwich structure of biovine serum albumin-biotin, avidin and biotinylation cells on the bioaffinity HMDS lattice array, thereby directionally distributing cells and realizing the separation and locating of cells. The materials used in the method are simple, and the method is simple and convenient; the distribution of the cell arrays can be realized by controlling the size and distribution mode of the HMDS; the method is used for patterning various cells; single-cell arrays are not easily broken in the following detection and application of the chip; and the materials and biochemical substances have small influence on the activity of cells, thereby being used for detecting living cells.

Description

technical field [0001] The invention belongs to the technical field of biological microsystems, and in particular relates to a cell patterned chip based on an avidin-biotin system and its preparation and application. Background technique [0002] The traditional drug screening method determines the key biological substance that causes the disease by tracking the biochemical pathway of animal tissue pathology, and then uses the target molecule of this substance for drug screening, which has the advantages of complex technology, low throughput, many animal experiments, large drug consumption, and high cost. shortcoming. Micrographic technology not only provides an alternative to expensive and time-consuming animal experiments for drug screening research, but also raises the research level to the level of cells and even genes. The high-throughput feature greatly shortens the cycle of drug screening and new drug development. At the cellular level, micropatterning technology can...

Claims

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Application Information

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IPC IPC(8): C12M1/34C40B40/02C40B50/06
Inventor 任大海尤政夏亦秋王俊谢锦宇
Owner TSINGHUA UNIV
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