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Application and method of fluorine oil as solvent for reducing water sensitivity of liquid phase biological sample for terahertz wave detection

A biological sample, water-sensitivity technology, applied in the field of medical testing, can solve the problems of insufficient water sensitivity, strong absorption of THz waves, and limited effect.

Active Publication Date: 2017-10-20
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Among the three technical means mentioned above, the THz wave frequency band optimization and reflective detection methods are only ways to optimize the THz signal extraction, the effect is limited and it does not fundamentally solve the strong absorption of THz waves by water, while the microfluidic technology is limited The influence of the manufacturing process and the internal friction of the microchannel can reduce the thickness of the water layer around the sample to millimeters or hundreds of microns at most, which is not enough to effectively eliminate the problem of "water sensitivity"

Method used

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  • Application and method of fluorine oil as solvent for reducing water sensitivity of liquid phase biological sample for terahertz wave detection
  • Application and method of fluorine oil as solvent for reducing water sensitivity of liquid phase biological sample for terahertz wave detection
  • Application and method of fluorine oil as solvent for reducing water sensitivity of liquid phase biological sample for terahertz wave detection

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1. Optimization of conditions for flushing biological samples with fluorine oil to replace surrounding water molecules

[0037] The condition optimization method for flushing biological samples with fluorine oil to replace surrounding water molecules, the specific steps are as follows:

[0038] (1) Taking tumor cells as the biological material model, the concentration of 5ml was 1.5×10 6 The cell / ml breast cancer MDA-MB-231 cell suspension was dropped on polyethylene (PE) sheets to make single-layer adherent cell slides;

[0039] (2) Clamp the cell slide into a detection fixture with a sample inlet and a sample outlet (the liquid sample detection pool of Bruker, Germany, model: A145, purchase route: Bruker), from the inlet to the outlet Fluorine oil was injected into the microfluidic syringe pump, the PE sheet was flushed at a flow rate of 20 μl / min, and the THz spectrum detection results of the sample were recorded every 1 min.

[0040] Test results such as ...

Embodiment 2

[0041] Example 2, using fluorine oil as a solvent to detect tumor cells

[0042] The method of using fluorine oil as a solvent to detect tumor cells, the specific steps are as follows:

[0043] (1) The concentration of 5ml is 1.5×10 6 The cell / ml breast cancer MDA-MB-231 cell suspension was dropped on a polyethylene (PE) sheet to make a single-layer adherent cell slide;

[0044] (2) Clamp the cell slide into a detection jig with a sample inlet and a sample outlet (same as Example 1). Experimental group: Inject fluorine oil from the injection hole with a micro-flow syringe pump to wash the PE sheet with or without adherent cells at a flow rate of 20 μl / min for at least 5 minutes; Wash the PE sheet with or without adherent cells at a flow rate of 20 μl / min for at least 5 minutes;

[0045] (3) Close the sample inlet and sample outlet of the fixture, and perform THz spectrum detection.

[0046] Test results such as Figure 5 shown. The results showed that when the cells were...

Embodiment 3

[0047] Embodiment 3, utilize fluorine oil as solvent detection bacterium

[0048] The method of using fluorine oil as a solvent to detect bacteria, the specific steps are as follows:

[0049] (1) Pick a single bacterial colony on the blood plate after pure culture and apply it evenly on a polyethylene (PE) sheet by the glass slide spin coating method to make a bacterial smear;

[0050] (2) Clamp the bacterial smear into a detection fixture (same as Example 1) with a sampling hole and a sampling hole, experimental group: inject fluorine oil from the sampling hole with a micro-fluid injection pump to have bacteria Wash the smears with a flow rate of 20 μl / min for at least 5 minutes; control group: inject PBS buffer solution from the injection hole with a microfluidic syringe pump, wash the smears without bacteria at a flow rate of 20 μl / min for at least 5 minutes;

[0051] (3) Close the sample inlet and sample outlet of the fixture, and perform THz spectrum detection.

[0052]...

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Abstract

The invention relates to application and a method of fluorine oil as a solvent for reducing the water sensitivity of a liquid phase biological sample for terahertz wave detection, by utilization of the principle that the fluorine oil has very low absorption in THz frequency band, and can replace solvent water with very high absorption around a biological substance, the problem of the water sensitivity of the liquid phase biological sample for terahertz wave detection can be solved, the invention also discloses a method for fixing a to-be-tested substance in the to-be-tested biological sample in a detection device, using the fluorine oil for scouring the sample for replacement of water molecules around the substance, and finally performing THz spectrum detection, the method has the advantages of simple operation, can reduce the signal loss of a THz detection signal passing through the liquid sample, improves the detection sensitivity, and has important significance for the application of THz spectroscopy in the biomedical field.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to the application of fluorine oil as a solvent for reducing the water sensitivity of liquid-phase biological samples detected by terahertz waves, and also relates to a method for detecting biological samples using fluorine oil as a solvent for liquid-phase biological samples detected by terahertz waves . Background technique [0002] Terahertz waves refer to electromagnetic waves with a frequency of 0.1 to 10 THz, between infrared and microwave, and are the transition band from macroelectronics to microphotonics. In recent years, with the gradual maturity of THz wave generation and detection technology and the great expansion of application fields, the research and development and application directions of THz technology at home and abroad have undergone extensive and profound changes. Due to its unique electromagnetic wave characteristics, the focus of terahertz research has shifte...

Claims

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Application Information

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IPC IPC(8): G01N21/3581G01N1/34
CPCG01N1/34G01N21/3581
Inventor 杨柯府伟灵杨翔
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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