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Nano photoelectronchemical sensor for detecting dopamine with high sensitivity

A sensitive detection, photoelectrochemical technology, applied in the field of analysis and detection, to achieve the effect of good selectivity and high sensitivity

Inactive Publication Date: 2013-03-20
江苏天协医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To the best of our knowledge, so far, no photoelectrochemical method has been reported to measure dopamine

Method used

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  • Nano photoelectronchemical sensor for detecting dopamine with high sensitivity
  • Nano photoelectronchemical sensor for detecting dopamine with high sensitivity
  • Nano photoelectronchemical sensor for detecting dopamine with high sensitivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. 250μL of thioglycolic acid solution and 50ml of 0.02mol / L Cd(ClO 4 ) 2 After the solution is mixed, add 1mol / L NaOH solution to adjust the pH of the solution to 7, and after feeding high-purity nitrogen for 30 minutes, add 5ml of 0.1M NaOH solution 2 Se aqueous solution, continue to feed nitrogen gas and stir for 4 hours to obtain water-soluble CdSe nanomaterials;

[0019] b. Immerse the ITO glass sheet in a 2% PDDA polymer solution containing 2mol / L NaCl, rinse the surface with deionized water after 5 minutes, then immerse it in the water-soluble CdSe nanomaterial, and rinse it with deionized water after 5 minutes Clean the electrode surface. The prepared electrode can be stored at room temperature.

[0020] c. The prepared CdSe-modified electrode is used as a working electrode, immersed in an electrolyte solution composed of 0.1mol / L triethanolamine and 0.1mol / L phosphate buffer solution (pH=7.0), adding different concentrations of dopamine, first using Cyclic ...

Embodiment 2

[0022] a, 0.527g of cysteine ​​hydrochloride and 50ml of 0.02mol / L CdCl 2 After the solution is mixed, add 1mol / L NaOH solution to adjust the pH of the solution to 11, and after passing high-purity nitrogen for 30 minutes, add 5ml of 0.1M NaOH solution 2 S aqueous solution, continue to feed nitrogen and stir for 4 hours to obtain water-soluble CdS nanomaterials;

[0023] b. Immerse the ITO glass piece in 2% PDDA polymer solution containing 2mol / LNaCl, rinse the surface with deionized water after 10 minutes, then immerse it in the water-soluble CdS nanomaterial, and wash it with deionized water after 10 minutes electrode surface. The above steps were repeated 5 times to prepare an electrode decorated with 5 layers of CdS nanomaterials. The fabricated electrodes were stored at room temperature.

[0024] c. The prepared CdS-modified electrode is used as a working electrode, immersed in an electrolyte solution composed of 0.05mol / L ascorbic acid and 0.1mol / L phosphate buffer so...

Embodiment 3

[0026] a, 0.588g of trisodium citrate dihydrate and 50ml of 0.02mol / L Cd(NO 3 ) 2 After the solution is mixed, add 1 mol / L NaOH solution to adjust the pH of the solution to 7.5; after passing high-purity nitrogen gas for 30 minutes, add 5 ml of 0.1M NaHTe aqueous solution, and continue stirring and reacting with nitrogen gas for 4 hours to obtain water-soluble CdTe nanomaterials;

[0027] b. Immerse the ITO glass sheet in 2% PDDA polymer aqueous solution containing 2mol / L NaCl, rinse the surface with deionized water after 8 minutes, and then immerse it in the water-soluble CdTe nanomaterial, rinse with deionized water after 10 minutes Clean the electrode surface. The above steps were repeated 5 times to prepare an electrode modified by 5 layers of CdTe nanomaterials. The prepared electrodes can be stored at room temperature.

[0028] c. The prepared CdTe-modified electrode is used as a working electrode, immersed in an electrolyte solution composed of 0.05 mol / L triethylami...

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Abstract

The invention provides a nano photoelectrochemical sensor for detecting dopamine with high sensitivity. The dopamine can be deposited to the surface of an electrode by using a chemical oxidation or electrochemical oxidation method, so as to form a polymer film, so that the photocurrent intensity of a quantum dot is reduced. Compared with a most common method for measuring the dopamine by using electrochemical cyclic voltammetry, the selectivity in detection of dopamine is improved, the measurement is not interfered by common coexisting substance ascorbic acid and uric acid in relatively high concentration, and the nano photoelectrochemical sensor is the first application of a photoelectrochemical method in detection of dopamine. With the adoption of the nano photoelectrochemical sensor, the dopamine detection with high-sensitivity is realized, the linear range is 2*10<-10>mol / L to 2*10<-5>mol / L, and the detection limit is 1*10<-10>mol / L. With the adoption of the method, the detection result of the dopamine in human urine is satisfactory, so that the method has the possibility of being applied to detection of the dopamine in human urine.

Description

Technical field: [0001] The invention relates to the field of analysis and detection, in particular to water-soluble quantum dots as photoelectrochemical probes and their application in photoelectrochemical detection of dopamine in human urine. Background technique: [0002] Dopamine (DA) is an important neurotransmitter in the mammalian central nervous system. The concentration distribution of dopamine in a specific area of ​​the brain directly affects the coordination of pituitary endocrine performance and is closely related to neural activity. Changes or disorders of its content can lead to certain diseases such as Parkinson's disease and schizophrenia [R.M.Wightman, L.J.May, A.C.Michael, Anal.Chem., 1988, 60, 769A-779A; A.Heinz, H .Przuntek, G.Winterer, A.Pietzcker, Nervenarzt, 1995, 66, 662-669; C.Martin, Chem.Br., 1998, 34, 40-42; J.W.Mo, B.Ogorevc, Anal.Chem., 2001, 3, 1196-1202.]. Therefore, the study of DA analysis methods is of great significance for neurophysio...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/333
Inventor 王光丽焦焕军董玉明傅侠张梁波
Owner 江苏天协医疗器械有限公司
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