Modeling processing method of non-alcoholic fatty liver mouse model

Abstract

The invention discloses a modeling method of a non-alcoholic fatty liver mouse model, and the method is characterized by consisting of the following steps that (1) a healthy mouse is caught and first adaptively raised for 3 to 7 days in an animal house, a backing material and clean water in a mouse cage are changed every day, and basic fodder is fed during that period of time; (2) after adaptive raising, composite high-fat fodder is fed every day; (3) 5 percent CC14 liquid is injected into the abdominal cavity of the mouse two weeks later, the liquid carbon tetrachloride is diluted to 5 percent emulsion with deionized water, and the liquid which is injected into the abdominal cavity shall be newly prepared; the abdominal cavity injection state continues for the 28th day, and meanwhile the composite high-fat fodder is fed; and the model is obtained. The modeling method has the advantages of short time, high modeling success rate, extremely low mortality rate and low cost.

Description

technical field [0001] The invention relates to a modeling technique of an animal model, in particular to a novel technique of a nonalcoholic fatty liver mouse model. Background technique [0002] With the improvement of people's living standards, the dietary structure has changed significantly, and the incidence of fatty liver disease (also known as fatty liver), which is called "rich man's disease", has increased significantly, especially in Asia. It has become the second most common liver disease after viral hepatitis. Fatty liver is caused by a variety of factors. The main pathological changes are in the hepatic lobules. It is one of the common diffuse liver diseases. Clinically, fatty liver is divided into alcoholic fatty liver disease (AFLD) and nonalcoholic fatty liver disease (NAFLD) according to whether there is excessive alcohol intake. Non-alcoholic fatty liver disease is more complicated, if not controlled in time, it will lead to inflammation and liver fibrosi...

AI Technical Summary

Problems solved by technology

At the beginning, the appetite of the mice was relatively large, but because the content of lard is too high, it is easy to produce a sense of satiety and greasy feeling, which leads to the loss of appetite of the mice and a significant decrease in food intake, which prolongs the time for modeling

There is another disadvantage of the traditional modeling method, that is, the dosage of carbon tetrachloride is 10% per injection, 0.2ml each time

A high concentration of carbon tetrachloride can effectively cause damage to liver cells, but the mice are weak or in poor condition, and intraperitoneal injection of this dose can easily lead to sudden death of model animals

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