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Bivalent inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and preparation method thereof

A dual inactivated vaccine and porcine circovirus technology, which is applied in the direction of antiviral agents, virus antigen components, pharmaceutical formulations, etc., can solve the problems of failure to develop the dual vaccine, difficulty in control, mutual interference, etc., and achieve immunity Convenient and quick, reduce side effects, improve survival rate

Inactive Publication Date: 2013-03-13
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only reports in the literature that the injected piglets are injected twice, so it is suggested that PCV2 antigen and PPV antigen may interfere with each other when injected at the same time
Therefore, the dual vaccine of PCV2 and PPV has not been successfully developed.
In addition, PPV in domestic farms also has a certain degree of variation. The mutated PPV and PCV2 work together to make the sow reproductive disorder and piglet PWMS syndrome more difficult to control. Therefore, there is an urgent need for a single injection to prevent and treat sows Porcine circovirus type 2 and porcine parvovirus dual vaccine for reproductive failure in pigs, especially pregnant sows, and PWMS syndrome in piglets

Method used

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  • Bivalent inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and preparation method thereof
  • Bivalent inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and preparation method thereof
  • Bivalent inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation and inspection of the dual inactivated vaccine of embodiment 1PCV2, PPV

[0045] 1.1 Preparation of poisonous seeds for production

[0046] Preparation of PCV2SH strain: Dilute the virus seeds appropriately with the virus diluent (serum-free MEM medium), inoculate them on PK-15 cells (CCTCC, No. GDC0060) at a multiplicity of infection (M.O.I.) of 0.01, and incubate at 37°C for 30 minutes , add MEM cell maintenance solution containing 4% (v / v) calf serum and 2mmol / L D-glucosamine hydrochloride, culture at 37°C for 4 days, freeze and thaw 2 to 3 times, harvest virus, virus titer ≥ 10 6.5 TCID 50 / ml.

[0047] Preparation of PPV HN-2011 strain: Dilute the virus seeds appropriately with virus diluent (serum-free α-MEM medium), inoculate them in ST cells (CCTCC, number GDC0007) at a multiplicity of infection (M.O.I.) of 0.01, and cultivate them for 37 ℃ for 30 min, add MEM cell maintenance solution containing 1% (v / v) calf serum and 2 mmol / L D-glucosamine ...

Embodiment 2

[0100] Example 2 PCV2, PPV dual inactivated vaccine and the comparison of the immune effect of using two kinds of vaccines (PPV inactivated vaccine and PCV2 inactivated vaccine) to immunize piglets alone

[0101] 1. Materials

[0102] PCV2, PPV double inactivated vaccine, select the laboratory product among the embodiment 1 for use (batch number is trial Z001); PCV2 inactivated vaccine (SH strain), Pulaike Biological Engineering Co., Ltd. produces (batch number is 100903), virus The content is at least 10% before inactivation 6.5 TCID 50 / ml; PPV disease inactivated vaccine, Wuhan Zhongbo Biological Co., Ltd. (batch number is 101001), the virus content is at least 10% before inactivation 7.5 TCID 50 / ml.

[0103] 2. Design of animal experiments

[0104] 80 weaned piglets aged 21-28 days were selected and divided into 4 groups, 20 pigs in each group; each pig in the first group was injected with 2ml of PCV2 and PPV dual inactivated vaccine (batch number 001) in the neck mu...

Embodiment 3

[0110] Reproductive performance and growth of piglets after embodiment 3 PCV2, PPV dual inactivated vaccine immunization sow

[0111] 1. Materials

[0112] PCV2, PPV double inactivated vaccine, select the laboratory product among the embodiment 1 for use (lot number is trial Z001). PCV2 inactivated vaccine (SH strain), produced by Pulaike Bioengineering Co., Ltd. (batch number 100903); PPV disease inactivated vaccine, Wuhan Zhongbo Biological Co., Ltd. (batch number 101001).

[0113] 2. Design of animal experiments

[0114] Select 60 gilts aged 6-7 months and divide them into 3 groups with 20 gilts in each group. In the first group, each gilt was immunized with dual inactivated vaccine one month before mating, boosted 1 time after 14 days, and was immunized for the third time 1 month before delivery, with 2ml / head each time. In the second group, each gilt was immunized with PCV2 and PPV disease inactivated single vaccine at the same time 1 month before mating, and boosted i...

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Abstract

The invention relates to polyvalent vaccines for prevention and treatment of porcine infectious diseases, especially to a combined vaccine for treatment and prevention of porcine circovirus type 2 (PCV2) and porcine parvovirus. By selecting PCV2 and porcine parvovirus, the preparation method provided in the invention consists of: culturing PCV2, and conducting inactivation and concentration; culturing a porcine parvovirus, and performing inactivation and concentration; mixing the two antigenic components in proportion, and supplement an adjuvant to prepare the vaccine. The bivalent vaccine prepared in the invention is easy to use, more secure, and has an immune effect superior to that of combined use of two single vaccines.

Description

technical field [0001] The invention relates to a dual inactivated vaccine of porcine circovirus type 2 and porcine parvovirus and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus, while PCV2 is a pathogenic virus. In addition to causing piglet multisystem wasting syndrome (Postweaning Multisystemic Wasting Syndrome, PMWS), PCV2 can also cause reproductive disorders in sows. cause serious economic losses. [0003] Porcine parvovirus (PPV) is one of the main pathogens causing reproductive disorders in pigs. PPV infection mainly causes embryo and fetal death, sow abortion, stillbirth and mummified fetuses. In addition, PPV is also related to non-suppurative myocarditis, dermatitis, enteritis and viremia in pigs. In recent years, studies have found that PPV and PCV2 ...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/20A61K39/12A61K39/23
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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