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Method for breaking algae cells

A technology for algal cells and algal liquid, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microbial dissolution, etc., can solve the problems of limited application scope, difficult operation, and many steps, and achieves good wall breaking effect and equipment. Simple, easy-to-use effects

Inactive Publication Date: 2013-01-23
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for crushing algae cells in order to solve the above-mentioned technical problems such as numerous steps, difficult operation, and limited scope of application. The method can effectively crush algae cells and has the characteristics of high processing efficiency.

Method used

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  • Method for breaking algae cells

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Embodiment 1

[0028] A method for breaking algae cells, specifically comprising the following steps:

[0029] (1) Measure 200 ml of harvested algae liquid into a 1 L autoclave, and stir for 5 min at 120-130 r / min;

[0030] (2) Turn on the ozone generator and air compressor, and pass the mixed gas composed of ozone and air with a volume ratio of 1:150 of ozone and air into the high-pressure reactor at a total flow rate of 2 L / min, and continue Stir the algae liquid at a constant speed of 120-130 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0031] (3) Control the pressure of the high-pressure reactor to 0.6 MPa. When it reaches 0.6 MPa, maintain it for 1 to 2 s, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.6 MPa. When it reaches 0.6 Mpa, the exhaust valve of the high-pressure reactor is opened again to reduce the pressure to normal pressure, and the broken algae cells are obta...

Embodiment 2

[0046] A method for breaking algae cells, specifically comprising the following steps:

[0047] (1) Measure 200 ml of the harvested chlorella solution in a 1 L autoclave, and stir for 5 min at 120-130 r / min;

[0048] (2) Turn on the ozone generator and air compressor, and pass the mixed gas composed of ozone and air with a volume ratio of 1:150 of ozone and air into the high-pressure reactor at a total flow rate of 2 L / min, and continue Stir the algae liquid at a constant speed of 120-130 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0049] (3) Set the pressure to 0.6 MPa. When it reaches 0.6 MPa, maintain it for 1-2 s, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.6 MPa. When it reaches 0.6 Mpa, open the exhaust valve of the high-pressure reaction kettle again to reduce the pressure to normal pressure, and cycle the boosting and depressurization process 80 times...

Embodiment 3

[0052] A method for breaking algae cells, specifically comprising the following steps:

[0053] (1) Measure 200 ml of the harvested chlorella solution in a 1 L autoclave, and stir for 5 min at 120-130 r / min;

[0054] (2) Turn on the ozone generator and air compressor, and pass the mixed gas composed of ozone and air with a volume ratio of 1:150 of ozone and air into the high-pressure reactor at a total flow rate of 2 L / min, and continue Stir the algae liquid at a constant speed of 120-130 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0055] (3) The set pressure is 0.8 MPa. When it reaches 0.8 MPa, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.8 MPa. When it reaches 0.8 MPa, open the high-pressure reactor again The exhaust valve of the kettle reduces the pressure to normal pressure, and the crushed algae cells are obtained after 80 cycles of increasing and decre...

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Abstract

The invention discloses a method for breaking algae cells. The method is characterized by firstly culturing algae species and acquiring algae liquid, then putting the harvested algae liquid into a reaction kettle and stirring the algae liquid uniformly, pumping a mixed gas of ozone and air in a volume ratio of 1:150 and stirring the substances uniformly, adding the pressure to 0.4-0.8MPa and maintaining the pressure for 1-2 seconds, opening an exhaust valve of the reaction kettle to release the pressure to the normal pressure so that the supersaturated gas is released from the algae liquid and repeating the processes of stirring the substances uniformly, pumping the mixed gas of ozone and air, adding pressure, releasing the pressure and releasing the supersaturated gas from the algae liquid for 40-80 cycles, thus completing breaking of the algae cells. The method has the following beneficial effects that the ozone is taken as a medium, acts on the algae liquid through gas-liquid reaction and is uniformly mixed with the algae liquid under the action of a stirrer and then breaks the algae cells; and the method has the characteristics of simple equipment, convenience in operation, good wall breaking effect and easiness in realization of automatic control.

Description

technical field [0001] The invention relates to a method for crushing algae cells, more specifically a new method for crushing chlorella cells by utilizing the joint action of ozone oxidation and pressurization-decompression. Background technique [0002] Human production and life produce a large amount of wastewater containing nutrients such as nitrogen and phosphorus. These wastewater enter lakes, rivers, bays and other water bodies through municipal drainage pipe networks and other systems, causing algae to multiply rapidly, the dissolved oxygen content in the water body decreases, and the water quality deteriorates. , which in turn leads to the mass death of fish and other organisms. In recent years, the deterioration of the environment caused by pollution has gradually increased, and the degree of algae pollution in water bodies has also deepened year by year. When eutrophication occurs in the water body, due to the large number of algae and other plankton, the water b...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/89
Inventor 黄远星李亮李洋洋许智华金小颖崔晨晨宋翠红方学友
Owner UNIV OF SHANGHAI FOR SCI & TECH
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