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Breeding method of erythromycin-producing strain mutagenesis by injecting plasma into spore bud

A technology of ion implantation and spore buds, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low mutation rate, weaken the mutagenic effect of mutagens, and fail to meet production needs, etc., to achieve High mutation rate, the effect of improving production efficiency

Inactive Publication Date: 2013-01-02
NINGXIA QIYUAN PHARMA
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AI Technical Summary

Problems solved by technology

However, because of the physiological characteristics of the spores, the spores have certain stress resistance to the mutagen, thereby greatly weakening the mutagenic effect of the mutagen on the genetic material, resulting in a low mutation rate and unable to meet production needs

Method used

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Examples

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Embodiment Construction

[0017] 1. Breeding steps: starting strain (selecting and using the strain saccharopolyspora erythraea for production), spore germination culture, collection of spore buds, ion implantation mutagenesis, isolation and culture, picking a single colony and connecting the slope, shaking the flask for investigation , fermentation culture, statistical analysis of experimental data, preliminary screening and retention of seeds.

[0018] 2 Mutagenesis screening process:

[0019] 2.1 Select the well-grown working cell bank slope used in large-scale production, add 30ml of sterile water, scrape the spores with an inoculation loop, suspend them in sterile water, and make a uniformly mixed spore suspension. Filter with sterilized filter paper, and collect the filtered spore liquid for later use.

[0020] 2.2 Spore germination culture.

[0021] 2.2.1 Physiological characteristics of spore buds. The spore germination is to absorb water and expand first, and then grow a germ tube, and the ...

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Abstract

The invention relates to a breeding method of erythromycin-producing strain mutagenesis by injecting plasma into spore buds. The method is characterized in that cultivated saccharopolyspora erythraea spore buds are employed for plasma injection for mutagenesis. According to the invention, plasma is injected into the spore buds for mutagenesis of erythromycin-producing strain, so as to realize high mutagenesis rate, and quickly and effectively obtain erythromycin strain with high positive mutation rate; and the strain can be stabilized and purified for production to effectively improve production efficiency.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a breeding method for erythromycin-producing bacteria induced by ion injection into spore buds. Background technique [0002] The strength of the strain directly affects the fermentation titer. Therefore, breeding a high-quality strain is of great significance for the fermentation of erythromycin. [0003] The conventional mutagenesis selection method of erythromycin-producing bacteria is: first prepare a spore suspension of a certain concentration, and the spore suspension is to scrape the inclined plane grown to the spore state with sterile water to make a suspension, and then use various mutagenic treatment with physical and chemical mutagens. This mutagenesis method is aimed at mutagenizing spores. Since spores are cells with reproductive and dormant functions produced during the growth and reproduction of bacteria, their vegetative cells can res...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00C12R1/645
Inventor 李学兵郭惠芬陈荔杨玉芳蒋鸿
Owner NINGXIA QIYUAN PHARMA
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