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Detecting method for hexavalent chromium in gelatin and products thereof

A detection method, the technology of hexavalent chromium, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems of non-detection of hexavalent chromium and low recovery rate, Achieve the effect of solving color interference, high recovery rate and simple pre-treatment

Active Publication Date: 2012-12-26
青岛谱尼测试有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Find through the inventor's test that the above method is used to measure the colored gelatin hollow capsule and has obvious deficiencies:
[0012] (3) Utilize above-mentioned method ③ the method in the thesis, need to add a large amount of activated carbon just can fully decolorize, but have serious absorption to the hexavalent chromium in the extract solution, the recovery rate is very low
[0013] At present, there is no method for detecting hexavalent chromium in gelatin and its gelatin products. The present invention aims to establish a simple, fast and effective method for determining hexavalent chromium in products

Method used

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  • Detecting method for hexavalent chromium in gelatin and products thereof
  • Detecting method for hexavalent chromium in gelatin and products thereof
  • Detecting method for hexavalent chromium in gelatin and products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1.1 Extraction of samples

[0030] Accurately weigh 2.00 g of crushed red gelatin hollow capsules, add 1 mL of 2mol / L phosphate buffer (2mol / L phosphate buffer: pH 9.63±0.1) and Mix 80mL of water, shake in a water bath at 80°C for 1h, cool, transfer to a 100mL colorimetric tube, and dilute to the mark with distilled water. Centrifuge at 8000 rpm for 10 min at room temperature and filter with a 0.45 μm filter membrane.

[0031] 1.2 Purification of samples

[0032] Take 15 mL of the extract after passing through a 0.45 μm filter membrane, adjust the pH value to 12.5 with 1 mol / L KOH, then slowly add 100 mg of GCB, vortex and mix for 1 min, let stand until the color of the solution fades obviously, and centrifuge at room temperature at high speed (≥8000rpm) 10min to detect the absorbance of the solution, the absorbance at 540nm is less than 0.1. Then pass through a 0.22 μm filter membrane.

[0033] 1.3 Color rendering

[0034] Accurately pipette 5mL of the above filtr...

Embodiment 2

[0046] According to the experimental procedure described in Example 1, the extract of a certain blue gelatin hollow capsule was tested by adjusting different pH values, and the results are shown in Table 1.

[0047] After treating the blue hollow capsules according to step (1.1), take 15mL of the extract, add Cr(VI) standard solution (equivalent to the concentration of the actual sample is 0.5mg / kg), adjust the pH value with 1mol / L KOH In the range of 8-14, add 50 mg of graphitized carbon black, vortex and mix for 1 min, centrifuge, and pass through the membrane. Follow step (1.3) for color development.

[0048] The influence of table 1pH value on actual sample

[0049]

[0050]

[0051] For the decolorization process of a certain blue gelatin hollow capsule extract, the amount of graphitized carbon black added is 50mg, and when the concentration of the standard is 0.5mg / kg, the pH value is adjusted to be greater than or equal to 12.5 in the experiment, and the recovery...

Embodiment 3

[0053] According to the experimental procedure described in Example 1, after adjusting the pH of the extract of the same blue gelatin hollow capsule, investigate the impact of the addition of graphitized carbon black on the recovery rate, and the detection results are shown in Table 2.

[0054] After treating the blue hollow capsules according to step (1.1), take 15 mL of the extract, add Cr(VI) standard solution (equivalent to the actual sample concentration of 0.5 mg / kg), and adjust the pH value with 1 mol / L KOH to 12.6. Slowly add graphitized carbon black, vortex and mix for 1 min, and let it stand until the color of the solution fades obviously. Centrifuge at room temperature at high speed (≥8000rpm) for 10 min to detect the absorbance of the solution, and the absorbance at 540nm is less than 0.1. Then pass through a 0.22 μm filter membrane. Follow step (1.3) for color development. When adding 50mg, 100mg, 150mg of graphitized carbon black, after passing through the membr...

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Abstract

The invention discloses a detecting method for hexavalent chromium in gelatin and products thereof, particularly relates to the detecting method for the hexavalent chromium in colored gelatin products. The method includes the following steps: using phosphate buffered solution to extract; adjusting PH value of the extraction solution to >=12.5; directly adding graphitized carbon black powder in the extraction solution with colorant for purification and decoloration; in acid medium, hexavalent chromium oxidation diphenyl carbonyl two hydrazine forms soluble amaranthine compounds. The ultraviolet spectrophotometry method is adopted to detect the content of the hexavalent chromium. The detecting method has the advantages that under the condition of highly alkalinity, the recovery rate of the hexavalent chromium can be guaranteed. Moreover, the graphitized carbon black powder can carry out sufficient decoloration for pigment in samples and avoid color interference. The detecting method is high in recovery rate, simple in pretreatment, low in cost, quick and accurate in result.

Description

technical field [0001] The invention relates to a detection method of hexavalent chromium, in particular to a detection method of hexavalent chromium in gelatin and its products. Background technique [0002] Hexavalent chromium is highly toxic to the human body and persistently harmful to the environment. It is 100 times more toxic than trivalent chromium and is easily absorbed by the human body and accumulates in the body. Functional failure, significant carcinogenic, teratogenic, reproductive toxicity and other effects. Gelatin products, such as gelatin empty capsules, are usually refined from medicinal gelatin plus auxiliary materials. The Chinese Pharmacopoeia has strict product standards for its products, and the limit of chromium is 2mg / kg. Because some unscrupulous gelatin manufacturers illegally use leather scraps "blue vitriol skin" as raw materials to produce industrial gelatin, pretend to be edible gelatin or medicinal gelatin to manufacture gelatin hollow capsu...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 宋薇
Owner 青岛谱尼测试有限公司
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