Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof
A chitosan and biological enzyme technology, applied in the fields of medical science, absorbent pads, prostheses, etc., can solve the problems affecting the normal tissue growth of the receptor, improve the stability and mechanical properties, reduce the production cost, expand the application and Application-wide effects
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Embodiment 1
[0031] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:
[0032] 1) Preparation of solution:
[0033] Weigh 2g of gelatin and add it to 50mL of deionized water, stir in a water bath at 50°C for 1 hour until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.04g / mL; weigh 1g of chitosan and add it to 50mL of 1% (volume percentage v / v%) acetic acid solution, stirred at room temperature for 3 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly added 1mol / L sodium hydroxide solution dropwise to the chitosan solution to The pH of the solution was 5.5; the microbial transglutaminase was added to deionized water and stirred at room temperature for 10 minutes, and then filtered to obtain a 2U / mL microbial transglutaminase solution; the tyrosinase was added to PBS (pH7. 0) ...
Embodiment 2
[0047] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:
[0048] 1) Preparation of solution:
[0049] Weigh 3g of gelatin and add it to 50mL of deionized water, stir in a water bath at 40°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06g / mL; weigh 1.5g of chitosan and add it to 50mL of 1% ((volume percentage v / v%) acetic acid solution, stirred at room temperature for 4 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.03 g / mL, and slowly added dropwise 0.5 mol / L hydroxide to the chitosan solution Sodium solution until the pH of the solution is 5.5; add microbial transglutaminase to deionized water and stir for 15 minutes, then filter to obtain a 2U / mL microbial transglutaminase solution; add tyrosinase to PBS (pH7 .0) buffer solution, stirred at room tempe...
Embodiment 3
[0055] A method for preparing a blended biological material of gelatin and chitosan modified by dual biological enzymes, comprising the following specific steps:
[0056] 1) Preparation of solution:
[0057] Weigh 6g of gelatin and add it to 100mL of deionized water, stir in a water bath at 60°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06g / mL; weigh 2g of chitosan and add it to 100mL of 1% ((volume percentage v / v%) acetic acid solution, stirred at room temperature for 5 hours until the chitosan was fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly added dropwise 0.8mol / L sodium hydroxide to the chitosan solution solution until the pH of the solution is 5.5; add microbial transglutaminase to deionized water and stir at room temperature for 20 minutes, then filter to obtain a 2U / mL microbial transglutaminase solution; add tyrosinase to PBS (pH7. 0) buffer solution, sti...
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