Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases

A technology for Alzheimer's disease and active ingredients, which is applied in the field of medicine to improve health quality and quality of life, improve cognitive function and improve safety.

Inactive Publication Date: 2014-04-02
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the application of forsythin in the preparation of drugs for improving cognitive function and treating Alzheimer's disease at home and abroad. Therefore, research and development use forsythin to protect brain nerve cells, regulate the secretion of brain neurotransmitters, and improve cognitive function. And the treatment of Alzheimer's disease is extremely valuable

Method used

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  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases
  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases
  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Experimental study of forsythin interfering with cognitive decline in aged mice

[0022] 1.1 Animals

[0023] There were 20 1-month-old ICR mice and 80 8-month-old ICR mice, half male and half male.

[0024] 1.2 Drugs

[0025] Forsythin was purchased from Nanjing Zelang Pharmaceutical Technology Co., Ltd., Jiangsu, and its purity was higher than 98% by HPLC.

[0026] 1.3 Test equipment

[0027] The composition of the Morris water maze: The water maze consists of three parts: a circular pool (diameter 90cm, height 50cm), platform and recording system. Arbitrarily divide the pool into four quadrants (northeast, southeast, southwest, and northwest). At the beginning of the experiment, the pool was filled with water to a depth of 30 cm, and 1 catties of milk powder was added to make the water opaque and milky white, and the water temperature was kept at about 24°C±1°C. There are abundant spatial reference objects (lights, cameras, operators, etc.) around the...

Embodiment 2

[0045] Example 2: Improvement of forsythin on cognitive decline in rats with Alzheimer's disease induced by hippocampal injection of Aβ

[0046] 2.1 Animals

[0047] 50 SD rats (8-10 months old, half male and half male, body weight 380-400 g) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0048] 2.2 Drugs and reagents

[0049] Forsythin is the same as Example 1;

[0050] Aβ1-42 was purchased from Sigma-Aldrich Company.

[0051] 2.3 Grouping

[0052] The animals were free to eat and drink water, the room temperature was 20°C-22°C, and the relative humidity was 60%-70%. / kg), 10 in each group, half male and half male. Three days after model establishment, intragastric administration was started; behavioral tests were carried out 6 weeks later.

[0053] 2.4 Aβ Rat Model Injected into the CA1 Region of the Hippocampus

[0054]Rats with normal learning and memory function were screened by platform jumping test. Aβ1-42 dimethyl sulfoxide...

Embodiment 3

[0063] Example 3: Effects of forsythin on central monoamine neurotransmitters in the brain of aged mice

[0064] 3.1. Instruments

[0065] HPLC detection system: binary pump, chromatographic column, automatic sampler, electrochemical detector.

[0066] 3.2 Drugs and reagents

[0067] NE, DA, 5-HT, 5-HIAA: purchased from Sigma-Aldrich Company.

[0068] HPLC grade perchloric acid, acetonitrile: Fisher Scientific.

[0069] 3.3 Detection method

[0070] 3.3.1 Specimen preparation

[0071] The mice after the behavioral test in Example 1 were decapitated, and the whole brain was quickly taken out on the ice table, weighed, quickly frozen in liquid nitrogen, and stored at -80°C. During extraction, the whole brain was homogenized in ice-cold 0.1mol / L perchloric acid for 20s (perchloric acid 1mL was added per 0.1g brain weight). 0.04% (w / v) Na in perchloric acid 2 S 2 o 5 and 0.04% (w / v) EDTA. The brain homogenate was centrifuged at 14000×g for 20 min at 4°C. The supernatant...

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Abstract

The invention discloses novel application of forsythin belonging to active components of natural medicinal plants, and particularly relates to application of forsythin as the only active ingredient to preparation of a medicine for improving a cognitive function and treating Alzheimer's diseases. Animal and cell research results indicate that the forsythin has the exact function of improving the cognitive function and can remarkably improve the spatial learning and memory ability of animals in an aging and Alzheimer's disease model, the level of a center monoamine neurotransmitter of an old-aged animal is increased, the genetic expression of presenilin 2 in a rat brain of an Alzheimer's disease model is reduced, and the survival rate of neuroblastoma cells induced by beta-amyloid protein is increased. The forsythin is matched with relevant auxiliary materials, a health care product or a medicine for purposefully improving the cognitive function and treating the Alzheimer's diseases can be prepared by a conventional preparation method, and the health care product and the medicine can be used for delaying and improving relevant diseases of cognitive hypofunction such as the course of the Alzheimer's diseases, enhancing the healthy quality and the life quality of old people and realizing a healthy aging social value.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of forsythin, an active ingredient of natural medicinal plants, as the only active ingredient in the preparation of drugs for improving cognitive function and treating Alzheimer's disease. Background technique [0002] Forsythiaside (CAS No. 487-41-2) is a medicinal plant active ingredient, and its sources include Forsythia suspensa (Thunb.) Vahl, a commonly used traditional Chinese medicine of the Oleaceae plant recorded in the Pharmacopoeia of the People's Republic of China. of dried fruit. [0003] With the deepening of the aging population, aging cognitive decline and Alzheimer's disease (Alzheimer's disease, a neurodegenerative disease, commonly known as senile dementia) have become more and more serious problems in Chinese society. Factors that affect cognitive decline in the elderly include demographic factors (mainly age, education level, etc.), and health and dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P25/28
Inventor 杨洋
Owner SUZHOU UNIV
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