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Method for bringing forward sexual maturity of cock

A mature, rooster technology, applied in the field of animal husbandry, that can solve problems such as little research on livestock and poultry

Inactive Publication Date: 2012-11-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The regulation of the Kisspeptin / GPR54 system on the reproduction of higher animals has become a hot spot in the field of reproductive biology research, but the research is mainly concentrated on humans, mice and fish animals, and there is little research on livestock and poultry

Method used

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  • Method for bringing forward sexual maturity of cock
  • Method for bringing forward sexual maturity of cock
  • Method for bringing forward sexual maturity of cock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Experimental animals Select 600 breeding eggs of Isha brown layer hens, and divide them into 3 groups randomly before hatching, disinfect the small head of the breeding eggs, use a toad spinal cord needle to gently drill a hole in the disinfected part, and use a micro-injection sample 100μL normal saline (control group, Con group); 0.5ng / 100μL Kp-10 (low dose group, L group); 2ng / 100μL Kp-10 (high dose group) dosage group, group H), and finally sealed with paraffin, and placed in an incubator for incubation. Standardized feeding was performed after hatching, and the fasting body weight was recorded weekly during the feeding period.

[0017] (2) Sample collection Blood was collected from the wing veins of roosters at the age of 14 weeks, 24 weeks and 30 weeks, and the serum was taken and stored at -20°C for testing. Collect rooster semen at 30 weeks old, dilute it with semen diluent, and observe the sperm count and sperm motility with a hemocytometer under an electr...

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Abstract

The invention belongs to the field of livestock raising and discloses a method for bringing forward the sexual maturity of a cock. The method for bringing forward the sexual maturity of the cock comprises the following steps that before the hatching, the small end of a hatching egg is sterilized, a hole is slightly drilled in the sterilized part by a toad spinal cord puncture needle, a trace sample feeder is used for introducing 100 muL 0.5 to 2ng / muL kisspeptin-10 injection reagents into egg white through the drilled hole, and an opening is sealed with paraffin wax. The kisspeptin-10 is used for processing the hatching chicken egg for the first time, in addition, the processing concentration of kisspeptin-10 is optimized, results show that when the method provided by the invention is used for Kp-10 (kisspeptin-10) egg injection, the body weight of the cock can be obviously increased, the sperm density of the cock is improved, the sexual behavior of the cock is increased, and the obvious influence is realized on the T content in the blood serum of the 14-week and 24-week cock.

Description

technical field [0001] The invention belongs to the field of animal husbandry and relates to a method for advancing the sexual maturity of roosters. Background technique [0002] At present, with the development of the economy and the continuous improvement of people's living standards, consumers consume more and more broilers and eggs, thus promoting the rapid development of the modern chicken industry. The fertilization rate and hatching rate of chicken breeding eggs are a key step in determining the breeder farm and the chicken industry. More and more studies have shown that the hatching rate of chicken farms largely depends on the fertilization of the breeder rooster. Ability, the early sexual maturity of the rooster is extremely important to the realization of the breeding benefits of the hen and the impact on the production performance of the offspring. With the continuous improvement of breeding technology, separate feeding of male and female has become a conventiona...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K45/00
Inventor 倪迎冬付文艳吴晶董海波赵茹茜
Owner NANJING AGRICULTURAL UNIVERSITY
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