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Spirulina beta-renieratene extract and preparation method thereof

A technology of carotene and spirulina, which is applied in food preparation, food science, drug combination, etc., can solve the problems of not meeting the demand of β-carotene and low content of β-carotene extract, and achieve the relief of symptoms of overeating, The effect of shortening the time

Active Publication Date: 2012-10-03
ORDOS XINYULI SPIRULINA IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content of β-carotene extract obtained by ordinary extraction method is low, which cannot meet the demand for β-carotene in the market

Method used

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  • Spirulina beta-renieratene extract and preparation method thereof
  • Spirulina beta-renieratene extract and preparation method thereof
  • Spirulina beta-renieratene extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Extraction of β-carotene: Take 0.5g of dried spirulina powder, and 12.5mL of methanol-acetone mixed solution (3:2) for ultrasonic vibration treatment at 51% power for 17min at 40°C.

[0025] 2) Purification of β-carotene extract: take 20mL of wet resin NKA-2, wash it with water until there is no alcohol smell. Take 100mL of the prepared crude extract, add the supernatant to the chromatographic column, adjust the sample loading rate to 1.0mL / min, and stop sample loading when the effluent is close to the saturated solution. Wash the column with distilled water for residual impurities.

[0026] 3) Next, desorb with acetone-methanol mixed solution at a flow rate of 1 mL / min, collect fractions every 5 min and detect the carotene concentration with an ultraviolet spectrophotometer. When the effluent reaches 40mL, start to collect the β-carotene extract until the effluent is colorless. The collected extracts were evaporated under reduced pressure to obtain crystals and st...

Embodiment 2

[0028] 1) Extraction of β-carotene: Take 10Kg of dried spirulina powder and treat it with 250L methanol-acetone mixed solution (3:2) at 51% power for 30min at 40°C.

[0029] 2) Resin X5 pretreatment for industrial purification of β-carotene extract: after fully soaking and swelling with 95% ethanol, wash with distilled water until there is no turbidity. Vacuum dry at 60°C for 40-50 hours.

[0030] 3) Purification of β-carotene extract: Take 40L of the treated resin X5 and wash with water until there is no alcohol smell. Take 2000L of the prepared crude extract, add the supernatant to the chromatographic column, adjust the sample loading rate to 1.0mL / min, and stop sample loading when the effluent is close to the saturated solution. Wash the column with distilled water for residual impurities.

[0031] Next, use acetone-methanol mixed solution to desorb at a flow rate of 1 mL / min, and collect fractions every 5 min to detect the concentration of β-carotene extract with a UV sp...

Embodiment 3

[0033] Extract animal (mice) activity assay. After several normal mice were fed for 7 days, the mice were randomly selected and divided into a normal control group and a normal administration group. After the remaining mice were fasted for 12 hours, a one-time intraperitoneal injection of streptozotocin (STZ) solution (STZ concentration of 30 mg / ml, prepared with citric acid buffer, pH 4.5) was used to establish a hyperglycemia model, as a model mouse They were randomly divided into negative control group and model administration group. The number of mice in each group was equal.

[0034] The CMC suspension of Spirulina β-carotene extract was administered to mice at a dose of 0.2 mL at 100 mg / kg, and the fasting blood lipids, body weight, food intake and water intake of the mice were measured for 10 consecutive days.

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Abstract

The invention discloses a method for extracting beta-renieratene with an in-vivo hyperglycemic active function. The method comprises the following steps of: performing ultrasonic-assisted extraction on spirulina powder serving as a raw material, and extracting by optimizing an organic solvent mixing proportion to obtain a crude beta-renieratene extract; and purifying by using a porous adsorption resin to obtain a beta-renieratene extract. Due to the adoption of the method, the yield of beta-renieratene can be increased effectively, and the product has the effects of lowering blood sugar and relieving polydipsia symptom, can be applied to functional foods and nutrient health products, and belongs to the technical fields of modern food processing and biology.

Description

technical field [0001] The invention relates to a preparation method for obtaining beta-carotene extract from spirulina. The extract has hypoglycemic activity and belongs to the field of modern food processing and biotechnology. Background technique [0002] Spirulina (Spirulina platensis) is recommended by the Food and Agriculture Organization of the United Nations as "the most ideal food in the 21st century". This type of algae is rich in protein, polysaccharides, unsaturated fatty acids, nucleic acids and trace elements, and the carotenoids (0.2-0.4%) it contains are 10-15 times that of other plants including carrots. Spirulina can be used as an important source of high-quality carotene. [0003] Beta-carotene is a type of carotenoid. It is ubiquitous in nature and is an important food coloring and nutritional enhancer. According to a number of researches at home and abroad, natural β-carotene has anti-oxidation, anti-tumor, radiation protection, and immunity-enhancin...

Claims

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Application Information

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IPC IPC(8): C07C403/24A61P3/10A23L1/30
Inventor 任迪峰陈林房明鲁军孙冰洁
Owner ORDOS XINYULI SPIRULINA IND GRP
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