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Method for building model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for human body

A bioavailability and model establishment technology, applied in the field of model establishment for joint evaluation of food cadmium accumulation on human bioavailability and toxicity, can solve the problems of bias, inaccuracy, and complicated operation in toxicity evaluation, and it is easy to achieve the test conditions. The effect of control, less pollution and simple operation

Inactive Publication Date: 2012-09-19
HENAN UNIV OF SCI & TECH
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Problems solved by technology

At present, the biological effects on the human body are evaluated indirectly mainly by measuring the accumulation of cadmium in food, which inevitably has great inaccuracies; animal experiments are also a method to evaluate food safety, but due to the existence of animal experiments The cycle is long, the operation is complicated, and it is easily affected by experimental factors (such as other feed ingredients, experimental conditions, animal physiological conditions, etc.), so there are often deviations in toxicity evaluation

Method used

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  • Method for building model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for human body
  • Method for building model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for human body

Examples

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Embodiment 1

[0024] Example 1: Bioavailability and toxicity of rice cadmium accumulation to human body:

[0025] (1) Collect rice grains, grind them, pass through pepsin digestion solution with pH 2.0, digest in 37°C constant temperature water bath for 1 hour; trypsin-bile extract digestion liquid with pH 7.0, digest in 37°C constant temperature water bath 2h; centrifuge, collect the supernatant, and autoclave the supernatant;

[0026] (2) Evenly mix the supernatant with the culture solution (without cadmium) to make a mixed solution, draw 2.5mL of the mixed solution and add it to the Caco-2 cell layer of the established joint culture model, and at the same time, draw the isotonic culture medium Add 2.5mL of solution to the 293T cell layer, and continue to culture in a carbon dioxide incubator at 37°C for 24 hours; if the food cadmium accumulation is low, a chronic toxicity test can be carried out, and the longest culture can be continued for 10 days;

[0027] (3) Collect Caco-2 cells and...

Embodiment 2

[0030] Example 2: Bioavailability and toxicity of animal liver cadmium accumulation to human body:

[0031] (1) Cook the animal liver, grind it, pass through the pepsin digestion solution with pH 2.0, and digest it in a constant temperature water bath at 37°C for 1 hour; trypsin-bile extract digestion liquid at pH 7.0, and digest it in a constant temperature water bath at 37°C Digest for 2 hours; centrifuge, collect the supernatant, and autoclave the supernatant;

[0032] (2) Evenly mix the supernatant with the culture solution (without cadmium) to make a mixed solution, draw 2.5mL of the mixed solution and add it to the Caco-2 cell layer of the established joint culture model, and at the same time, draw the isotonic culture medium Add 2.5mL of solution to the 293T cell layer, and continue to culture in a carbon dioxide incubator at 37°C for 24 hours; if the food cadmium accumulation is low, a chronic toxicity test can be carried out, and the longest culture can be continued f...

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Abstract

The invention relates to a method for building a model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for a human body, and the method comprises the following steps of (1) food sample digestion and determination liquid acquiring: a, grinding a food sample after the food sample is collected, and sequentially digesting the food sample by sequentially adopting gastric juice and intestinal juice; and b, centrifuging the digested mixed liquid, collecting supernate to be sterilized through high pressure; (2) independently culturing Caco-2 cells and 293T cells; (3) combined culture of Caco-2 cells and 293T cells: moving an insertion groove with the Caco-2 cells into a hexagonal-porous plate with the 293T cells to be continuously cultured for 24h; (4) evaluation of bioavailability and toxicity of cadmium: placing the food sample determination liquid on a Caco-2 cell layer of a combined culture model, simultaneously adding iso-osmotic incubation liquid on a 293T cell layer, and continuing the culture for 24h; in the chronic toxicity test, maximally collectively culturing the sample for 10d; and (5) detecting indexes. The method has characteristics of simpleness in operation, easiness in controlling test conditions, small pollution, economical efficiency, accurate result and the like, and also has the advantages for collectively evaluating the bioavailability and toxicity. The method is suitable for evaluating the safety of the cadmium accumulation in grains, vegetables and animal food.

Description

technical field [0001] The invention relates to a method for establishing a model for jointly evaluating the bioavailability and toxicity of food cadmium accumulation on human body. Background technique [0002] Cadmium pollution has been widespread in the world, which leads to a certain amount of cadmium enrichment in human's main food, which poses a potential threat to human health. Food safety testing is an important guarantee to prevent human cadmium poisoning. At present, the biological effects on the human body are indirectly evaluated mainly by measuring the accumulation of cadmium in food, which inevitably has great inaccuracies; animal experiments are also a method to evaluate food safety, but due to the existence of animal experiments The cycle is long, the operation is complicated, and it is easily affected by experimental factors (such as other feed ingredients, experimental conditions, animal physiological conditions, etc.), so there are often deviations in tox...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N27/62G01N27/626
Inventor 何万领李晓丽张才李健张纪亮李旺李元晓杨盼盼
Owner HENAN UNIV OF SCI & TECH
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