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Method for rapidly determining anthocyanin content in blueberries

A blueberry anthocyanin, rapid determination technology, applied in the direction of measuring device, color/spectral characteristic measurement, material analysis through optical means, etc., can solve the problems of unsatisfactory, chemical pollution, low analysis precision, etc., and achieve high accuracy , high accuracy and reliability, fast analysis speed

Inactive Publication Date: 2012-08-22
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former has low analytical precision and cannot determine the content of a single component of anthocyanin; the latter has high analytical precision, but the analysis process is complicated and slow, and it may cause chemical pollution during the pretreatment of the sample, which cannot meet the production requirements. Requirements for on-line component content monitoring in the process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The anthocyanin content of Elliott, a very late-maturing variety of Beigaocong, was selected. First, use the Antarise Fourier Transform Near Infrared Spectrometer to collect the spectrum at room temperature, the sampling mode is integrating sphere diffuse reflection, and the spectral range is 4000cm. -1 ~10000cm -1 , Scanning times 32, resolution 8cm -1 , InGaAs detector collects the signal. Each group of samples was scanned 3 times, and the average value was taken as the analysis spectrum. When sampling, the samples should be evenly and tightly arranged in the sample slot to prevent the beam from leaking. Input the collected spectrum into the built model, and read the content of anthocyanin directly. The obtained Elliott anthocyanin content ranges from 2.33 to 3.72 mg / g fresh weight, with an average value of 3.13 mg / g fresh weight.

Embodiment 2

[0014] Darrow, a late-maturing variety of Beigaocong, was selected to determine its anthocyanin content. First, use the Antarise Fourier Transform Near Infrared Spectrometer to collect the spectrum at room temperature, the sampling mode is integrating sphere diffuse reflection, and the spectral range is 4000cm. -1 ~10000cm -1 , Scanning times 32, resolution 8cm -1 , InGaAs detector collects the signal. Each group of samples was scanned 3 times, and the average value was taken as the analysis spectrum. When sampling, the samples should be evenly and tightly arranged in the sample slot to prevent the beam from leaking. Input the collected spectrum into the built model, and read the content of anthocyanin directly. The obtained darnocyanin content ranged from 1.53 to 2.25 mg / g fresh weight, with an average value of 1.81 mg / g fresh weight.

Embodiment 3

[0016] The mid-high cluster late-maturing variety Northblue was selected to determine its anthocyanin content. First, use the Antarise Fourier Transform Near Infrared Spectrometer to collect the spectrum at room temperature, the sampling mode is integrating sphere diffuse reflection, and the spectral range is 4000cm. -1 ~10000cm -1 , Scanning times 32, resolution 8cm -1 , InGaAs detector collects the signal. Each group of samples was scanned 3 times, and the average value was taken as the analysis spectrum. When sampling, the samples should be evenly and tightly arranged in the sample slot to prevent the beam from leaking. Input the collected spectrum into the built model, and read the content of anthocyanin directly. The obtained northern cyanidin content ranged from 1.52 to 2.89 mg / g fresh weight, with an average value of 2.05 mg / g fresh weight.

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Abstract

The invention relates to a method for rapidly determining the anthocyanin content in blueberries by using near infrared spectroscopy (NIRS). The method includes: establishment of a model: principal component analysis and partial least squares are adopted; and spectral scanning: under the room temperature, an Antarise Fourier transform near-infrared spectrometer is adopted to acquire a spectrogram, the sampling mode is integrating sphere diffuse reflection, the spectral region is 4000cm-1 to 10000cm-1, the scanning frequency is 32, the resolution is 8cm-1, and signals are acquired by an InGaAs detector. Samples are blueberries, the acquired spectrogram is inputted into the established model, and the content of anthocyanin is rapidly read out. The method is applicable to the same model of near-infrared spectrometers and the determination of the anthocyanin of different varieties of blueberries. The accuracy reaches 92.7 percent, and the method has the characteristics of rapidness and nondestructiveness.

Description

Technical field [0001] The invention relates to a method for rapidly detecting the content of anthocyanins in blueberries by using near infrared spectroscopy technology (NIRS). Background technique [0002] The anthocyanins contained in blueberries have a good health care effect on the eyes, can reduce eye fatigue and improve night vision. The human eye can see objects due to the presence of retinoin on the retina, which decomposes opsin and retinal coloring substances under the stimulation of light to produce neurotransmitting substances to transmit to the brain. Resin is repeatedly decomposed, synthesized, and continuously transmitted to the brain. The important function of blueberry anthocyanins is to activate and promote the resynthesis of retinoin, thereby improving the acuity of human vision and accelerating the adaptation to the dark environment. Taking advantage of this characteristic of blueberries, the internationally developed health food for increasing the reputatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 孙爱东王姗姗陈健陶晓赟李路宁王寅张师
Owner BEIJING FORESTRY UNIVERSITY
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