Cylinder plate method-based leather material bacteriostasis effect test method
A detection method, the cup and saucer method, is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of lack of antibacterial effect detection methods and less research on antibacterial performance, so as to achieve difficult observation and recording, Simple operation and accurate results
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[0034] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.
[0035] 1. Materials
[0036] 1.1 Sample: Leather from Fuhua Leather Co., Ltd., Quzhou, Zhejiang (anti-mold treatment, no anti-mold treatment)
[0037] 1.2 Culture medium: beef extract peptone solid medium, beef extract peptone semi-solid medium, beef extract peptone liquid medium
[0038] 1.3 strains
[0039] Escherichia coli 1.358; Staphylococcus aureus 1.801; Bacillus subtilis 1.108.
[0040] 1.4 Drugs and reagents
[0041] 2,3,5-Triphenyltetrazolium chloride (TTC), NaCl, etc. are domestic analytical grade
[0042] 1.5 Vessels and Instruments
[0043] Petri dishes, test tubes, Erlenmeyer flasks, pipettes, pipette guns, suction balls, inoculation loops;
[0044] Bacteria counting plate; electronic analytical balance; vacuum drying oven; binocular microscope; biochemical incubator; ultra-clean workbench; vertical pressure steam...
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