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Quantitative determination method of component in pill of six ingredients with rehmannia

A quantitative detection method, the technology of Liuwei Dihuang Pills, is applied in the field of quality detection of Liuwei Dihuang Pills, which can solve the problems of cumbersome operation steps, high inspection costs, and limited supply, and achieve simplified inspection processes, simple preparation methods, and reduced inspection costs Effect

Inactive Publication Date: 2014-03-12
LIAONING PROVINCE INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Taking the classic drug Liuwei Dihuang Pills as an example, the main components of Liuwei Dihuang Pills are 5-hydroxymethylfurfural, morroniside, loganin, paeoniflorin and paeonol. The reference substance solution of the components, and then prepare the test solution respectively, and then detect according to different detection conditions. , loganin, paeoniflorin and paeonol, the reference substance prices are high, especially the supply of morroniside reference substance is extremely limited, the price of morroniside per 20mg is more than 2000 yuan, resulting in high inspection cost, the current traditional Chinese medicine inspection field The technology development trend is fast, efficient, simultaneous determination of multi-component content

Method used

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  • Quantitative determination method of component in pill of six ingredients with rehmannia
  • Quantitative determination method of component in pill of six ingredients with rehmannia
  • Quantitative determination method of component in pill of six ingredients with rehmannia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Liuwei Dihuang Pills Control Chinese Patent Medicine Extract

[0033] (1) Preparation of Liuwei Dihuang pill control Chinese patent medicine extract

[0034] 1) Preparation of Liuwei Dihuang Pills as a control Chinese patent medicine: take 160 g rehmannia glutinosa, 80 g wine cornus, 60 g moutan cortex, 80 g yam, 60 g Poria cocos and 60 g Alisma, grind them into fine powder, pass through a 40-mesh sieve, Mix well; add 100g of refined honey to every 100g of powder to make Liuwei Dihuang Pills as a Chinese patent medicine;

[0035] 2) Preparation of Liuwei Dihuang Pills control Chinese patent medicine extract: Take 15g of Liuwei Dihuang Pills as a control Chinese patent medicine, put it into a stoppered Erlenmeyer flask, add 200mL of methanol, ultrasonically extract for 25 minutes, filter, and add the filtrate to a neutral alumina column (100-200 mesh, 10g, inner diameter 1cm), elute with 100mL methanol, collect the eluate, evaporate to 50mL, transfer to a 10...

Embodiment 2

[0055] Example 2 Detection of Commodity Contents of Commercialized Liuwei Dihuang Pills

[0056] 1) The Liuwei Dihuang Pill prepared in Example 1 was used as the standard substance for the detection of the control Chinese patent medicine extract;

[0057] 2) Processing of commercial Liuwei Dihuang Wan samples: Weigh 1 g of 26 batches of commercial Liuwei Dihuang Wan from different manufacturers in the market, put them in a stoppered Erlenmeyer flask, add 30 mL of methanol, extract by ultrasonic for 25 minutes, filter, and evaporate the filtrate to to 5mL, transfer to a 10mL volumetric flask, add methanol to the mark, and prepare the test solution;

[0058] 3) Detection: High performance liquid chromatography is used for detection, and the detection conditions are as follows:

[0059] Chromatographic column: octadecyl bonded silica gel as filler;

[0060] Mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.05% phosphoric acid water;

[0061] Gradient elution:...

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Abstract

The invention relates to a quantitative determination method of pills of six ingredients with rehmannia, and a preparation method and application thereof. The adopted technical scheme is as follows: each millilitre of the contrast Chinese patent medicine extract of the pills of six ingredients with rehmannia contains 16.71-66.82 mu g of 5-hydroxymethylfurfural, 30.56-122.22 mu g of mrroniside, 19.52-78.06 mu g of loganin, 12.71-50.84 mu g of paeoniflorin, and 40.27-161.06 mu g of paeonol. The content determination of the pills of six ingredients with rehmannia is carried out by taking the contrast Chinese patent medicine extract of the pills of six ingredients with rehmannia provided by the invention as the standard substance and adopting a high-erformance liquid chromatography; therefore, the method is capable of simultaneously controlling a plurality of components, simplifying a checkout process and greatly reducing checkout cost and meets with the technology development tendency in the traditional Chinese checkout field; furthermore, the preparation method of the contrast extract is simple and convenient and is beneficial to being popularized and applied and has strong practicability.

Description

technical field [0001] The invention belongs to the field of component analysis of traditional Chinese medicines, and in particular relates to a quality detection method for commercialized Liuwei Dihuang Pills. Background technique [0002] As we all know, a large number of reference substances are often used in the inspection of Chinese patent medicines. The high price and limited quantity of reference substances have become an important factor restricting the wide application of reference substance inspection methods. At the same time, the cost of production and inspection of enterprises has increased, resulting in high commodity prices. one of the factors. [0003] In addition, the Pharmacopoeia stipulates that for the measurement items of the content of Chinese medicine components: for the simultaneous determination of the content of two or more components of Chinese patent medicines, it is necessary to prepare two or more corresponding reference substance solutions in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 郝延军桑育黎孙苓苓李虹陈婧王维宁赵晓笠徐秋阳
Owner LIAONING PROVINCE INST FOR FOOD & DRUG CONTROL
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