Calcium-resistant soyabean protein and preparation method thereof
A soybean protein and calcium tolerance technology, applied in the field of soybean protein, can solve problems such as poor calcium tolerance, and achieve the effect of reducing co-precipitation, weakening electrostatic interaction and high purity
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Embodiment 1
[0026] The low-temperature defatted soybean meal (NSI=89, protein content 51%) was pulverized and passed through a 60-mesh sieve to obtain the defatted soybean powder, mixed with water according to the mass-volume ratio of 1:10, added 0.08% sodium sulfite by weight of the soybean powder, and heated at room temperature and pH8 0 under the condition of stirring for 1.5 hours, then homogenized 20min in the colloid mill, the obtained defatted soy flour slurry was figure 1 Jet cooking is carried out in the continuous heat treatment device shown, the treatment temperature is 140°C, and the time is 90s. After the obtained heat treatment slurry is cooled, the pH value is adjusted to 8.0 with 2mol / L NaOH, centrifuged at 25°C for 30min, and the centrifugal speed is 3000rpm. The final supernatant was adjusted to pH 4.5 with 2mol / L HCL, left to stand for 5 minutes, and centrifuged at 25°C for 30 minutes at a speed of 5000 rpm. ℃, air outlet temperature 85 ℃), to obtain calcium-tolerant so...
Embodiment 2
[0038] The defatted soybean meal (NSI=89, protein content 51%) was crushed and passed through a 60-mesh sieve to obtain the defatted soybean powder, mixed with water according to the mass-volume ratio of 1:10, adding 0.15% sodium sulfite of soybean powder weight, and using 2mol / L NaOH adjusted the pH value to 8.0 and stirred at room temperature for 2 hours, then homogenized in a colloid mill for 10 minutes, and the obtained defatted soybean flour slurry was figure 1 Jet cooking treatment is carried out in the continuous heat treatment device shown, the temperature and time are respectively 120°C, 90s (E) and 140°C, 30s (F), the obtained heat treatment slurry is cooled, adjusted to pH 8.0, and centrifuged at 25°C for 30min, the centrifugal speed After centrifugation, the supernatant was adjusted to pH 4.5 with 2 mol / L of HCL. After standing for 5 minutes, it was centrifuged at 25°C for 30 minutes at a speed of 5000 rpm. The resulting precipitate was re-dissolved in water, adjust...
Embodiment 3
[0040] The soybean protein raw materials obtained in the above-mentioned Examples 1, 2 and Comparative Example 1 are mixed with corresponding ingredients to prepare calcium-enriched soybean protein liquid food. Maltodextrin, 0.4g sodium citrate and 0.2g calcium chloride (the amount of calcium added is based on the amount of calcium ions, which is 0.9% of the weight of protein raw materials), add 64.9g water and stir well, and use 2mol / L HCL and NaOH Adjust the pH to 7.0, then add 2.5g of corn oil and homogenize at a speed of 20000rpm for 2 minutes, then homogenize the mixture twice at 50MPa, and finally sterilize the mixture in a sterilizing pot at 121°C and 0.1MPa. Bacteria 20min. Among them, the properties and states of each sample before and after sterilization were measured and evaluated, and the results are shown in Table 2.
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