Method for carrying out simultaneous quantitative analysis on four lignan components in Chinese magnoliavine raw material and Chinese magnoliavine extract

A technology of schisandrin A and schisandrin B, which is applied to the analysis of materials, measuring devices, and material separation, can solve the problems of incomplete application and imperfection of analysis conditions, and achieve the effect of simple pretreatment process and high extraction efficiency

Inactive Publication Date: 2012-04-18
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Abstract
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  • Application Information

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Problems solved by technology

[0024] At present, the quality control of Schisandra chinensis is mainly based on a single or a few components. Even if the same component is measured for different analytical instruments, the analytical conditions are not fully applicable, and it is necessary to adjust or re-explore the analytical method.
And at present, the method research reports for determining the best high-performance liquid chromatography analysis conditions in different analytical instruments are not perfect, and there has not yet been about using a reversed-phase distribution column as a ZORBAX Extend-C18 (150 × 4.6mm, 5 μm) Relevant reports on quantitative determination of schisandrin A, schisandrin ester A, schisandrin A and schisandrin B in schisandra raw material and / or schisandra extract when methanol-0.1% trifluoroacetic acid aqueous solution is the mobile phase

Method used

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  • Method for carrying out simultaneous quantitative analysis on four lignan components in Chinese magnoliavine raw material and Chinese magnoliavine extract
  • Method for carrying out simultaneous quantitative analysis on four lignan components in Chinese magnoliavine raw material and Chinese magnoliavine extract
  • Method for carrying out simultaneous quantitative analysis on four lignan components in Chinese magnoliavine raw material and Chinese magnoliavine extract

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Experimental program
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Effect test

Embodiment 1

[0067] Embodiment 1: Determination of HPLC analysis conditions

[0068] In this embodiment, the Agilent high performance liquid chromatography system: quaternary pump G1311A, online degasser G1379A, autosampler G1329A, variable wavelength detection 1314A; reagents: methanol and acetonitrile are dedicated to HPLC, and water is deionized water.

[0069] 1. Selection of chromatographic column and mobile phase

[0070] Due to the complexity and diversity of traditional Chinese medicine ingredients, the determination of the analysis method must first be based on its characteristics, to find the chromatographic mode and the best column system. For the analysis of general medicinal materials or the total extract of the compound, choose the reversed-phase liquid chromatography mode, and establish a pure water reversed-phase To the analysis method of the full concentration range of traditional Chinese medicine high performance liquid chromatography non-aqueous reversed-phase, ionic and...

Embodiment 2

[0085] Embodiment 2: quantitative correlation test

[0086] The standard solution of preparation is changed sample size by above-mentioned analysis condition and carries out chromatographic analysis, the chromatographic peak area obtained and solution concentration are done linear regression analysis, and the regression curve obtained has good linear relation ( Figure 9 ).

Embodiment 3

[0087] Embodiment 3: instrument precision experiment

[0088] Under the optimal chromatographic conditions, 25 μl of the reference solution was precisely drawn and injected in parallel for 10 times. It can be seen from the measurement results that the relative deviations of the four substances are all less than 3.0%, and the reliability of the measurement results can meet the analysis requirements.

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Abstract

The invention discloses a method for carrying out simultaneous determination on contents of schisandrin, schisantherin, deoxyschizandrin and schisandrin B in a Chinese magnoliavine raw material or a Chinese magnoliavine extract. The method employs means of crushing, sieving, methanol ultrasonic extraction, calibrating volume and filtering to treat schisandra chinensis or a Chinese magnoliavine extract to obtain a sample solution for testing; a high performance liquid chromatography is utilized for simultaneous determination on contents of four lignan components. The method utilizes a reverse direction distribution chromatographic theory to carry out analysis on the sample by gradient elution, under conditions of a column temperature of 25-45 DEG C, mobile phases of methanol and 0.05-0.2% trifluoroacetic acid aqueous solution (methanol and trifluoroacetic acid aqueous solution in a volume ratio of 50:50-100:0), an elution time of 30-60 min, a flow velocity of 0.7-1.5 ml / min and a detection wavelength of 210-280 nm. The quality detection method is simple, rapid, sensitive and accurate.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a method for quantitative analysis of traditional Chinese medicine components, in particular to a method for quantitatively analyzing schisandra chinensis alcohol A, schisandra chinensis ester A, schisandrin A, Schisandrin content method. Background technique [0002] Schisandra chinensis is commonly known as mountain pepper, weighing tuozi, medicine schisandra, noodle vine, five plums, etc., and is the dry and mature fruit of Magnoliaceae Schisandra chinesnsis Baill. Tang et al.'s "Xinxiu Materia Medica" contains "the five flavors are sweet and sour in the skin and flesh, and hard in the core, all of which have a mature flavor", so it is named Schisandra. Ancient medical books call it Chuzhu, Xuanji, and Huiji, which were first listed in the top grade of Shennong’s Herbal Classic. The efficacy of traditional Chinese medicine lies in the power of nourishing an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 肖清贵周玲徐红彬孙玉良王学魁张懿
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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