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Detection method for soybean water-soluble proteins

A technology of water-soluble protein and detection method, applied in the field of soybean water-soluble protein detection, can solve the problems of unstable linear fitting degree of photoelectric signal, limit detection accuracy, etc. The effect of improving detection efficiency

Active Publication Date: 2013-09-18
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Coomassie Brilliant Blue method is simple, fast, and sensitive, but the photoelectric signal is unstable and linear fit limits its detection accuracy

Method used

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  • Detection method for soybean water-soluble proteins
  • Detection method for soybean water-soluble proteins
  • Detection method for soybean water-soluble proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take soybeans with a mass of 0.020 g, and take samples from the dorsal side of the hilum along the direction parallel to the hilum, and the sampling amount is 0.01-0.07 g / grain. The test results of the relationship between sampling amount and germination are shown in Table 1.

[0051] Table 1 The relationship between soybean grain intake and germination

[0052] Check weight

Germination rate

germination variance

detection value

Variance of detection value

0.01g / grain

100.00％

0.00％

31.33％

1.37％

0.02g / grain

100.00％

0.00％

31.45％

0.99％

0.03g / grain

95.00％

2.65％

31.48％

0.83％

0.04g / grain

90.33％

3.06％

31.46％

0.73％

0.05g / grain

83.33％

4.04％

31.57％

0.62％

0.06g / grain

80.33％

4.16％

31.36％

0.55％

0.07g / grain

65.33％

5.51％

31.62％

0.51％

[0053...

Embodiment 2

[0090] Detection of water-soluble protein in soybean variety Ji nf58:

[0091] Clamp the soybean seeds with needle-nose pliers, use a clean file along the direction parallel to the hilum, and file part of the cotyledons into powder on the back side of the hilum, and the rest can continue to grow seedlings. The weight is 14.5g, and the weighing part accounts for 1 / 7 of the soybean single grain weight).

[0092] Weigh 0.020 g of soybean powder (accurate to 0.001 g) and place it in a 2 mL centrifuge tube, add 2 mL of distilled water, and shake on a shaker at 20° C. for 15 min. Remove the centrifuge tube, centrifuge at 3000r / min for 10min, take the supernatant and place it in a 10mL volumetric flask. Repeat the extraction of the residue, oscillate on a shaker at 22°C for 30 minutes, remove the centrifuge tube, centrifuge at 3500r / min for 10 minutes, mix the extraction solution, and finally dilute to 10mL with distilled water to prepare a water-soluble protein test solution.

[0...

Embodiment 3

[0101] The offspring 138 population of Jidou 12 crossed with Ji nf58 was used as the test material to test the correlation and consistency of the detection results of the Kjeldahl method provided by the present invention.

[0102] Detection method provided by the invention:

[0103] Clamp the soybean seeds with needle-nose pliers, use a clean file along the direction parallel to the hilum, and file part of the cotyledons into powder on the dorsal side of the hilum, and continue to raise seedlings for the rest, and file 0.020g from a single seed (the weighed part accounts for about 1 / 10 of the single grain weight of soybean).

[0104] Weigh 0.030 g of sample powder and place it in a 2 mL centrifuge tube, add 2 mL of distilled water, and shake on a shaker at 22°C for 15 min. Remove the centrifuge tube, centrifuge at 3500r / min for 5min, take the supernatant and put it in a 10mL volumetric flask, repeat the leaching of the residue for 30min, and finally adjust the volume to 10mL ...

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Abstract

The invention relates to the technical field of biology, in particular to a detection method for soybean water-soluble proteins. In the method, the soybean sampling way is improved, so that the requirement of detection on water-soluble proteins in early generation soybeans is met, normal seedling raising of the residual part is not influenced, and low generation screening and high generation heredity analysis are realized. In the detection method, a reaction system is reduced, so that the proportion of a protein sample to a coomassie brilliant blue dye is changed, and the linear fitting degree of the detection method is increased. The detection method is suitable for detecting samples of which the sample sizes are small.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting soybean water-soluble protein. Background technique [0002] The protein content in soybeans is about 38%, which is 4 to 5 times that of cereals. Its amino acid composition is similar to that of milk protein. Except for slightly lower methionine, other essential amino acids are rich in content. In terms of nutritional value, soybean protein is equivalent to animal protein. The arginine content in soybean protein drinks is higher than that in milk, and the ratio of arginine to lysine is also reasonable. The content of lipids and linoleic acid is extremely rich and does not contain cholesterol, which can prevent the occurrence of cardiovascular diseases in adulthood. In addition, soybeans are also rich in lecithin, which can remove excess sterols in the blood. [0003] In recent years, with the improvement of people's living standards, soybean protein, especial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 张孟臣唐晓东
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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