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Preparation method of nanometer graphene oxide carrier for photodynamic therapy

A technology of nano-graphite oxide and photodynamic therapy, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. It can solve the problem of low effective utilization rate of photosensitizer and low loading rate of photosensitizer. problem, to achieve good therapeutic effect

Inactive Publication Date: 2012-03-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the loading rate of these carriers on the photosensitizer is very low (1-2wt%) resulting in low effective utilization of the photosensitizer, so increasing the loading rate of the carrier on the photosensitizer is a problem to be solved by many researchers

Method used

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  • Preparation method of nanometer graphene oxide carrier for photodynamic therapy
  • Preparation method of nanometer graphene oxide carrier for photodynamic therapy
  • Preparation method of nanometer graphene oxide carrier for photodynamic therapy

Examples

Experimental program
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Effect test

Embodiment 1

[0021] In order to increase the loading rate of the nano-carrier to the photosensitizer and effectively apply to photodynamic therapy, the present invention provides a novel and efficient system for loading photosensitizers based on nano-graphene oxide. In particular, the surface of the nano-graphene oxide is The modification of hydrophilic polymer can be well dispersed in physiological environment such as physiological saline cell culture medium. Then through π-π stacking and hydrophobic-hydrophobic interactions, strong hydrophobic photosensitizer molecules can be efficiently loaded to form a new type of efficient photosensitizer loading system.

[0022] The following uses ammoniated monomethyl polyethylene glycol (mPEG-NH 2 ) Is a hydrophilic polymer modified graphene oxide, and zinc phthalocyanine (ZnPc) is used as a photosensitizer model to give a comprehensive and detailed introduction to the preparation, function and effect of the photosensitizer carrier of the present inven...

Embodiment 2

[0026] Phagocytosis experiment

[0027] The nano graphene oxide loaded with ZnPc was prepared according to the method of Example 1, and then the cell phagocytosis experiment was performed. Inoculate human breast cancer cells (MCF-7) in a six-well plate, discard the original medium after culturing for 24 hours, and add 1 mgL of ZnPc-loaded nano-graphene oxide DMEM solution -1 , And cultivate for another 24h. Observing the phagocytosis of the material by the cells with a fluorescence microscope, it can be found that the ZnPc red light excitation light is distributed in the cell nucleus and cell membrane, indicating that nanographene oxide can be phagocytosed by breast cancer cells.

Embodiment 3

[0029] Photodynamic therapy experiment

[0030] The nano graphene oxide loaded with ZnPc was prepared according to the method of Example 1. After the nano graphene oxide loaded with zinc phthalocyanine (ZnPc) was co-cultured with the cells, the photodynamic therapy was performed under the irradiation of a xenon lamp with filtered ultraviolet light Take effect as an example. Human breast cancer cells MCF-7 were seeded in a 96-well plate at 5000 cells / 100μL. After 24 hours of culture, the original culture medium was discarded, and the DMEM solution of ZnPc-loaded nano-graphene oxide was added to each well, and then cultured for another 24 hours. The xenon lamp with filtered ultraviolet light is 200mWcm -2 The power is irradiated for a few minutes, the total dose of light is 60Jcm -2 . After the irradiation, the DMEM solution of nano-graphene oxide was aspirated and fresh DMEM medium was added to continue the culture for 4 hours. After incubation, the cell viability was measured b...

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Abstract

The invention relates to a preparation method of a nanometer graphene oxide carrier for the photodynamic therapy. The method is characterized in that: the surface of nanometer graphene oxide, which is modified by a hydrophilic polymer, loads a hydrophobic photosensitizer containing a large pi-conjugated structure through the pi-pi accumulation and the hydrophobic-hydrophobic interaction, so a product for the photodynamic therapy is obtained. Compared with the prior art, the photosensitizer loading rate of the nanometer graphene oxide carrier, which is high to 14 wt%, is higher than the photosensitizer loading rate of other photosensitizer carriers, such as a polymer micelle, a liposome, a liposome, a nanometer silicon ball and the like, so the nanometer graphene oxide carrier which can be applied to the photodynamic therapy has a good treatment effect, and has an obvious inhibitive ability on the growth of cancer cells under the irradiation of near infrared light, so a novel high efficiency loading photosensitizer system is provided for the treatment of cancers of tumors at shallow skin surfaces.

Description

Technical field [0001] The invention relates to a method for preparing a nano carrier, in particular to a method for preparing a nano graphene oxide carrier for photodynamic therapy. Background technique [0002] Photodynamic therapy is to use a suitable carrier to load a photosensitizer into cancer cells or tissues. Under near-infrared light irradiation, reactive oxygen species are generated to destroy cancer cells or tissues. Recently, photosensitizers based on phthalocyanine or porphyrin have been rapidly developed in the research of photodynamic therapy. Since most photosensitizers are highly hydrophobic, they tend to aggregate in physiological environments and have low bioavailability. Therefore, the research based on the photosensitizer carrier has also attracted the attention of many researchers. Such as the literature (van Nostrum CF.Polymeric micelles to deliver photosensitizers for photodynamic therapy.Adv Drug Deliver Rev, 2004,56:9-16; Derycke ASL, de Witte PAM.Lipo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/04A61K47/34A61K47/42A61K47/36A61K47/38A61K9/14A61P35/00C01B31/04
Inventor 董海青李永勇徐梦沈爱军温慧云时东陆
Owner TONGJI UNIV
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