Method for detecting content of Danshensu in Fuyankang tablets

A technology of Fuyankang Tablets and determination methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of easy popularization, good repeatability, and high accuracy

Inactive Publication Date: 2012-01-18
湖南湘泉药业股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing literature only records the HPLC determination method of paeoniflorin, berberine hydrochloride and matrine in Fuyankang Tablets, but there is no report on the determination method of danshensu content

Method used

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  • Method for detecting content of Danshensu in Fuyankang tablets
  • Method for detecting content of Danshensu in Fuyankang tablets
  • Method for detecting content of Danshensu in Fuyankang tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Take 10 Fuyankang Tablets, remove the sugar coating from the sugar-coated tablets, grind finely, take about 1.0g, weigh it accurately, put it in a stoppered Erlenmeyer flask, and add methanol-0.5% glacial acetic acid solution (methanol and 0.5% Glacial acetic acid solution (volume ratio 1:1) 20ml, plugged tightly, weighed, ultrasonically treated (power 150W, frequency 40kHz) for 20 minutes, allowed to cool at room temperature, weighed again, and methanol-0.5% glacial acetic acid solution (including methanol and 0.5% glacial acetic acid solution (volume ratio 1:1) to make up for the lost weight, shake well, filter, and take the subsequent filtrate to obtain the sample solution of Fuyankang Tablets to be tested;

[0024] 2) Accurately weigh 12.58 mg of Danshensu sodium reference substance which has been dried under reduced pressure by phosphorus pentoxide for 12 hours, put it in a 25 ml measuring bottle, dissolve it with 70% methanol and dilute to the mark, and shake we...

Embodiment 2

[0028] Embodiment 2 repeatability test

[0029] Take the same batch number (batch number: 20080724) 6 samples of Fuyankang Tablet sample fine powder, follow the step (1) of Example 1 to prepare Fuyankang Tablet to be tested sample solution and operate in parallel, and accurately absorb the reference substance Solution (0.0503 mg / ml) and 10 μl of the sample solution of Fuyankang Tablet to be tested were injected into the liquid chromatograph for determination. The results are shown in Table 2. The results show that the method has good repeatability.

[0030]

Embodiment 3

[0031] Embodiment 3 accuracy test

[0032] Take the same batch number (080724) Fuyankang tablet sample powder (containing Danshensu 0.19mg / tablet) 6 parts, about 1.25g, accurately weighed, put into a 50ml measuring bottle, and accurately add the reference substance solution (0.0503 mg / ml) 25ml, operate in parallel according to the method under step (1) of Example 1, accurately draw 10 μl, inject it into a liquid chromatograph, and measure it. The results are shown in Table 3, and the results show that the recovery rate of this method is good.

[0033]

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Abstract

The invention relates to a method for detecting the content of Danshensu in Fuyankang tablets, which comprises the following steps: 1) preparing a sample solution to be measured of Fuyankang tablets; 2) drafting a standard curve of a comparison product solution of Danshensu; 3) injecting the obtained sample solution to be measured of Fuyankang tablets in a step 1) into a liquid chromatograph for chromatogram determination of Danshensu in Fuyankang tablets; 4) calculating the content of Danshensu in Fuyankang tablets according to the chromatogram of the obtained sample solution to be measured in a step 3) and the standard curve of the comparison product solution of Danshensu. The high performance liquid chromatography (HPLC) detection method for detecting the Danshensu content in Fuyankang tablets has advantages of simple sample treatment method, exclusive test method, good repeatability and high accuracy, and has significance, for controlling the product quality of the Fuyankang tablets.

Description

technical field [0001] The invention relates to a method for quality control in the field of pharmacy, in particular to an HPLC method for determining the content of danshensu in Fuyankang tablets. Background technique [0002] Fuyankang Tablet is a traditional Chinese medicine preparation recorded in the 2010 edition of "Chinese Pharmacopoeia". The content of matrine is the quality control standard. [0003] Existing literature only records the HPLC determination method of paeoniflorin, berberine hydrochloride and matrine in Fuyankang Tablets, but there is no report on the determination method of danshensu content. [0004] However, Danshen occupies a relatively large proportion in Fuyankang Tablets. Danshensu in Danshen is one of the active ingredients for promoting blood circulation and removing blood stasis. Determination of the content of Danshensu in Fuyankang Tablets can better reflect its function of regulating qi and promoting blood circulation , which is of consi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 王实强谢新平
Owner 湖南湘泉药业股份有限公司
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