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Dihydric alcohol and organic acid co-production and separation method

A separation method and organic acid technology, applied in the field of bioengineering, can solve the problems of single fermentation products, carbon dioxide emissions, and low utilization rate of raw materials, and achieve the effects of simplified separation process, easy scale-up and industrialization, and easy implementation

Active Publication Date: 2014-07-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of carbon dioxide emission, low raw material utilization rate and single fermentation product in the current 1,3-propanediol and 2,3-butanediol fermentation, the present invention proposes pressurized fermentation / carbonate to adjust pH-salting out extraction-CO 2 A New Method for Immobilization-Coupled Production of Glycols and Organic Acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Adding sodium carbonate solution to adjust the fermentation pH to realize the coproduction of 1,3-propanediol, lactic acid and succinic acid

[0027] (1) Bacterial species: Klebsiella pneumoniae (CGMCC 2028)

[0028] (2) Culture medium composition:

[0029] ①Seed medium (1L):

[0030] Glycerin: 20g; KH 2 PO 4 : 1.3g; CaCO 3 : 2g; K 2 HPO 4 ·3H 2 O: 4.454g; (NH 4 ) 2 SO 4 : 2.0g; MgSO 4 ·7H 2 O: 0.2g; Yeast powder: 1g; Trace element A: 2mL; Ca 2+ Solution: 1 mL; Fe 2+ Solution: 1 mL.

[0031] ②Fermentation medium (1L):

[0032] Glycerin: 40g; KH 2 PO 4 : 1.36g; Citric acid: 0.42g; MgCl 2 ·6H 2 O: 0.26g; (NH 4 ) 2 SO 4 : 6.61g; yeast powder: 1g; trace element B: 5mL.

[0033] ③ Fe 2+ Solution composition (100ml): saturated hydrochloric acid: 0.4ml; FeSO 4 ·7H 2 O: 0.5g;

[0034] ④Ca 2+ Solution (100ml): CaCl 2 : 2g.

[0035] ⑤ Composition of trace element A (1L):

[0036] Saturated hydrochloric acid: 0.9mL; MnCl 2 4H 2 O: 100...

Embodiment 2

[0042] Embodiment 2: Add sodium carbonate solution to adjust pH to realize 1,3-propanediol, succinic acid and lactic acid co-production

[0043] (1) Bacterial species: Klebsiella pneumoniae (CGMCC 2028)

[0044] (2) Substratum composition: fermentation medium yeast powder: 2g, other with embodiment 1.

[0045] (3) Fermentation control: 5L fermenter, fed-batch fermentation, liquid volume 3L, fermentation temperature 37°C, feed 95% industrial glycerin, initial concentration 40g / L, inoculum size 10% (v / v) , the stirring speed is 300r / min, the air volume is 0.02vvm, and the pH is adjusted to 7.0 by using 5mol / L sodium hydroxide solution and 2.5mol / L sodium carbonate solution respectively. 4 hours after the start of fermentation, detect the concentration of glycerol, add glycerol to control the concentration of glycerol at about 15-20g / L, and ferment until the end of 36 hours.

[0046] (4) Fermentation result: when adopting 5mol / L sodium hydroxide solution to adjust the pH, ferme...

Embodiment 3

[0048] Embodiment 3: Sodium carbonate / ethanol raffinate adjusts the 1,3-propanediol batch fed-batch fermentation of pH value

[0049] (1) Bacterial species: Klebsiella pneumoniae (CGMCC 2028)

[0050] (2) Culture medium composition: with embodiment 1.

[0051] (3) Fermentation control: 5L fermenter, batch fed-batch fermentation, liquid capacity 3L, fermentation temperature 37°C, stirring speed 200r / min, inoculum size 10% (v / v), air volume 0.02 vvm, 5mol / L sodium hydroxide solution and sodium carbonate / ethanol salting-out extraction system were used to adjust the pH to 7.0 during the fermentation process. Feed 95% industrial glycerin, the initial concentration is 40g / L, when the glycerin in the fermentation broth is consumed to a certain concentration (about 20g / ), start to feed glycerin, control the glycerin concentration at 15-25g / L during the fermentation process, and ferment for 36 hours Finish.

[0052] (4) Fermentation results: when using 5mol / L sodium hydroxide solution...

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Abstract

The present invention belongs to the field of bio-engineering technology, and provides a method for co-producing and separating diols and organic acids. The characteristics are that the object of study is Klebsiella pneumoniae for producing diols through bio-converting glycerol or glucose, etc., using pressurized fermentation and fermentation adjustment with carbonates, pH - salting out and extraction - CO2 immobilization coupling to produce diols and organic acids; 1,3-propanediol and 2,3-butanediol are separated from lactic acid and succinic acid by a two-step salting out and extraction. The present invention can achieve the co-production of different diols and organic acids in the same culture system by controlling different fermentation conditions, and enhance the utilization of raw materials; and at the same time recycle carbon dioxide generated from tail gas in fermentation, and solve the common problem of carbon dioxide emission in the fermentation industry. The present invention establishes a new high-efficiency and low-cost process integrated with fermentation - separation - CO2 immobilization, and provides references for CO2 recycling and co-producing other chemicals.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to microbial fermentation co-production and carbon dioxide recovery and utilization technology, in particular to a method for co-production and separation of dihydric alcohol and organic acid. Background technique [0002] Under the circumstances of expanding energy demand and unstable supply of petroleum resources, biorefinery technology using renewable biomass resources as raw materials has attracted more and more attention from researchers at home and abroad, especially the use of industrial biotechnology to produce biomass Energy and bio-based bulk chemicals, such as fuel ethanol, biodiesel, biogas, biohydrogen and 1,3-propanediol, 2,3-butanediol, lactic acid, succinic acid, butanol / acetone, etc. Among them, 1,3-propanediol, 2,3-butanediol, lactic acid and succinic acid have attracted much attention and are the most industrialized diol and dibasic acid fermentation products. B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/22
CPCC12P7/56C12P7/18C12N1/20C12P7/46C07C51/48C07C29/86
Inventor 孙亚琴魏搏超李志刚戴建英修志龙
Owner DALIAN UNIV OF TECH
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