Method for preparing camellia sinensis extract through dynamic/ static combined subcritical CO2 extraction

Abstract

The invention relates to a method for preparing a camellia sinensis extract through dynamic / static combined subcritical CO2 extraction, which belongs to the field of processing of natural products. The method provided in the invention comprises the procedures of selective picking of camellia sinensis, dehydration, fragmentation, selection of an entrainer, dynamic / static combined subcritical CO2extraction, fractionation, condensation, etc. According to the invention, extraction conditions for the method are mild; the method enables main aroma components in camellia sinensis like acetophenone, leaf alcohol, phenylethyl alcohol and alpha-phenylethyl alcohol to be effectively retained, polar and non-polar ingredients in aroma components of camellia sinensis to be highly effectively extracted and high extraction efficiency to be obtained; the obtained camellia sinensis extract can be directly used as a product and can also be further separated and purified for preparation of high-grade essence or champignon products, having a promising market prospect and development value.

Description

technical field [0001] The invention relates to an extraction method of natural plant aroma components, in particular to a method for preparing tea tree flower extract by combining dynamic and static subcritical CO2 extraction. Background technique [0002] Tea tree flower ( Camellia sinensis ) is a by-product of tea production. my country is rich in tea tree flower resources, with an area of ​​18 million mu of tea gardens, ranking first in the world, and 20kg to 30kg of dried tea tree flowers can be picked per mu. From this, it is estimated that tea regions across the country can produce more than 4.5 million tons of dried tea tree flowers every year. [0003] Tea tree blossoms are rich in protein, tea polysaccharides, amino acids, vitamins and other beneficial ingredients and active substances, which have detoxification, lipid-lowering, hypoglycemic, anti-cancer, nourishing, and beauty-enhancing effects on the human body; mature tea tree flowers secrete Honey juice is fr...

AI Technical Summary

Problems solved by technology

[0004] At home and abroad, there are very few extractions and researches on the aroma components of tea tree flowers, and traditional organic solvent extraction methods are mostly used, such as petroleum ether or ether to extract the aroma components of tea tree flowers, but the resulting aroma loss is serious and the yield is low.

[0005] In recent years, supercritical CO 2 Fluid extraction does not have these disadvantages, however, supercritical CO 2 The operating pressure of the extraction is high, which requires high equipment safety and stability, and the extraction process is easy to cause short circuit and bonding of the material layer, while the subcritical CO 2 The extraction operation conditions are milder, the safety and stability are higher, and the subcritical CO 2 The density ratio of supercritical CO 2 Larger, the solubility of fat-soluble substances is also greater, and the CO extracted from the same amount of tea tree flowers 2 The consumption is less and the cost is lower; on the other hand, CO 2 The polarity of the tea tree flower is low, and the extraction ability of the polar compounds in the aroma components is weak. Therefore, if the solvents with different polarities are selected as the entrainer combination according to the aroma composition of the tea tree flower, the unique aroma of the tea tree flower can be effectively maintained and the extraction efficiency can be improved; In terms of operating technology, the dynamic and static combined extraction process can loosen the cells of the tea tree flower tissue and increase the mass transfer between the solvent and the matrix, while the fractional separation process is based on the specific components of the tea tree flower extract in CO 2 Different from the solubility in the entrainer, the primary separation is achieved, and the two operating processes are designed based on the characteristics of the tea tree flower