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Method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in large scale

A technology of Dendrobium officinale and test-tube seedlings is applied in the field of plant cultivation, which can solve the problems of vitrification and large variation rate of seedlings, low transplanting survival rate, low seed germination rate, etc., and achieves shortened germination time and transplanting adaptability. Good, the effect of increasing growth rate

Inactive Publication Date: 2012-01-04
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing rapid seedling propagation process, usually the whole production stage adopts the stages of seed sowing into solid medium, subculture transfer, strong seedling rooting, etc. The whole production stage from sowing to strong seedling rooting is completely used as the light source by fluorescent lamp It leads to problems such as long production cycle, low seed germination rate, high seedling vitrification and mutation rate, high energy consumption, high production cost, and low transplanting survival rate. After the seedlings, they need to go through the seedling training stage before they can be transferred to the greenhouse for cultivation and planting.

Method used

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  • Method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in large scale
  • Method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in large scale
  • Method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in large scale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) On the ultra-clean workbench in the inoculation room, place the uncracked Dendrobium candidum capsules in a jar, pour 70% (v / v) alcohol into the bottle, soak for about 50s, and filter out the alcohol. Then soak in 0.1% (w / w) mercuric chloride aqueous solution for about 12 to 15 minutes, repeatedly shake horizontally during the soaking process, so that the surface of the capsules can be thoroughly disinfected, and then rinse repeatedly with sterile water for 3 to 4 times, and the sterilized capsules Cut open and take the seeds for later use.

[0028] (2) Dendrobium officinale seeds are sown in the liquid medium of 1 / 4MS medium+6-benzylpurine 0.2mg / L+naphthaleneacetic acid 0.2mg / L+potato juice 80g / L+sucrose 25g / L, pH 5.4; Put it into a constant temperature shaking incubator for shaking culture. The culture conditions of the shaking incubator are set at a temperature of 25°C, a rotation speed of 120-150r / min, an illumination of 300-1000 lux, and a light time of 12h / d. ...

Embodiment 2

[0033] Except that in step (2) of the embodiment 1, the seed is sown in the liquid medium and the vibration culture is changed to the solid medium for germination, and the step (5) the natural light and sunlight cultivation in the greenhouse is changed to the fluorescent lamp cultivation in the cultivation room, the others are the same as the embodiment 1; steps (2), step (3), step (4) in seed germination, strong seedling cultivation, strong seedling rooting cultivation three stages are screened respectively with the substratum combination listed in Table 1, to optimize the optimum substratum combination formula. Table 1 illustrates: ① The optimization of the medium formula is mainly to design different basic medium, hormone concentration, natural additives, activated carbon concentration, etc. to optimize the design of the medium. In this example, it is designed as a solid medium. The agar concentration was 5g / L, the sucrose was 25g / L, and the culture environment was a culture...

Embodiment 3

[0039] Others are the same as in Example 1, the Dendrobium officinale seeds obtained in step (1) are sown respectively in the following 4 different mediums: 1. 1 / 4MS medium+6-benzylpurine 0.2mg / L+naphthaleneacetic acid 0.2mg / L+potato Juice 80g / L+sucrose 25g / L+pH 5.4 liquid medium; ②1 / 4MS medium+6-benzylpurine 0.2mg / L+naphthaleneacetic acid 0.2mg / L+banana juice 100g / L+sucrose 25g / L+pH 5.4 liquid culture ③ MS medium + 6-benzylpurine 0.2mg / L + naphthaleneacetic acid 0.2mg / L + potato juice 80g / L + sucrose 25g / L + pH 5.4 liquid medium; ④ 1 / 4 MS medium + 6-benzylpurine 0.2mg / L+naphthaleneacetic acid 0.2mg / L+potato juice 80g / L+sucrose 25g / L+agar 5.0g / L+pH 5.4 solid medium. The culture environment of the liquid medium was set at a temperature of 25°C, a rotational speed of 120r / min, an illuminance of 1000 lux, and a light time of 12h / d, and the culture was shaken. The culture environment of the solid medium was set at a temperature of 25°C, an illuminance of 1000 lux, and a light tim...

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Abstract

The invention discloses a method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in a large scale. The method provided by the invention comprises the following steps of: disinfecting Dendrobium candidum capsules, rinsing and slicing off to obtain seeds; sowing seeds into a liquid culture medium and carrying out a shaking culture for 50-60 days; germinating seeds to form protocorms and further forming gemmules; putting the gemmules into a strong seedling culture medium to be cultured for 2-2.5 months to form plantlets with 2-3 leaves and 1-2 radicles; transferring the radicles into a strong seedling rooting culture medium and covering an opening sealing membrane on a bottle opening of a tissue bottle; transferring the tissue bottle to a plastic greenhouse seedling bed with an outer shade; providing sunlight or natural light and culturing for 2-3 months to obtain bigger seedlings; taking out the bigger seedlings from the bottle, washing, disinfecting and airing; then transplanting the bigger seedlings into cultivation matrix of a plastic greenhouse to be cultured for 24-30 months to obtain the product. The process provided by the invention can be directly used for industrially producing germchit in a large scale and has the advantages of less pollution, high seed germination rate, low variation rate, strong germchit, low production cost, high transplanting survival rate and the like.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for propagating test-tube seedlings of dendrobium officinale. Background technique [0002] Dendrobium candidum is an extremely rare and precious Chinese medicinal material recorded in the Chinese Pharmacopoeia. It is known as "Chinese Immortal Grass" and "Gold in Medicine". With the depletion of wild resources, the rising market demand for plants under national second-class protection has promoted the development of the artificial cultivation industry, and the high-quality seedling breeding link in industrial development has increasingly become the bottleneck of industrial development. In the existing rapid seedling propagation process, usually the whole production stage adopts the stages of seed sowing into solid medium, subculture transfer, strong seedling rooting, etc. The whole production stage from sowing to strong seedling rooting is complete...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 席刚俊史俊徐超赵桂华杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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