Nucleic acid dual fluorescence PCR (Polymerase Chain Reaction) detection kit for influenza A/B virus

Abstract

The invention provides a nucleic acid dual fluorescence PCR (Polymerase Chain Reaction) detection kit for an influenza A / B virus. The detection kit comprises an RNA (Ribonucleic Acid) enzyme inhibitor, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) liquid, an enzyme mixed liquor, an influenza A / B virus dual reaction liquid, a positive control and a negative control, wherein the influenza A / B virus dual reaction liquid comprises a component (1) which consists of a pair of primers for detecting the influenza A virus and a probe for detecting the influenza A virus and a component (2) which consists of a pair of primers for detecting the influenza B virus and a probe for detecting the influenza B virus; and the enzyme mixed liquor comprises a Taq enzyme and a reverse transcription enzyme. By adopting the detection kit, the influenza A and B viruses can be detected at the same time, and the problem that only one influenza virus can be detected in one reaction in the conventional product is solved. The detection kit has the advantages of easiness and convenience for operating, high repeatability, quick and objective detection result and the like, and has a great application prospect in the field of in-vitro diagnosis of influenza viruses.

Description

technical field [0001] The invention relates to a dual fluorescent PCR detection kit for simultaneous detection of type A / type B influenza virus nucleic acid, which belongs to the field of biotechnology. Background technique [0002] Influenza is a seasonal disease. In temperate regions, influenza prevails throughout the winter. In tropical regions, influenza viruses exist throughout the year, and are more common in rainy seasons. Common influenza A, commonly known as cold, is an acute respiratory infectious disease caused by influenza A virus as the pathogen, and the main mode of transmission is air droplet transmission. The severity of influenza is related to the individual immune status. Generally, only about 50% of infected patients will develop typical clinical symptoms of influenza. Typical symptoms of influenza include sudden fever, dizziness, headache, myalgia, mild systemic symptoms, and may be accompanied by sore throat and cough, nasal congestion, runny nose...

AI Technical Summary

Problems solved by technology

[0005]1) Virus isolation and culture have high technical requirements, are expensive, and time-consuming, and the positive rate of isolation varies from laboratory to laboratory, which cannot meet the requirements of virus epidemic at the same time. The need to handle a large number of samples, currently only used for experimental research

[0006]2) Serological methods cannot process samples with high throughput, and cannot accurately reflect whether they are currently infected or carry the virus

[0008] At present, most of the products adopt the detection method for a single virus. For the clinical detection of multiple viruses, it is necessary to perform multi-tube detection on one sample, which increases the detection Cost and complexity of operation

[0009] From the above, it can be seen that the commonly used virus isolation method is time-consuming, the serological diagnosis method has hysteresis, and the gene sequence determination method is also relatively complicated and cumbersome.

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