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Novel microbial wax removal and control system and application thereof

A wax and anti-microbial technology, applied in the direction of cleaning equipment, mining fluids, drilling compositions, etc., can solve the problems of serious environmental pollution, stratum pollution, high operating costs, etc., to improve economic benefits, reduce crude oil viscosity, improve The effect of mobility

Inactive Publication Date: 2011-06-15
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the four types of wax removal methods, electric heating wax removal is the most effective wax removal and anti-wax measure, but it has disadvantages such as high cost and unsafety.
Organic solvent wax removal mostly uses mixed benzene and light hydrocarbons, the effect of wax removal is very good, but the disadvantage is that it is flammable and explosive, and the price is high
Chemical cleaning and anti-wax have good effect, low cost, safety and reliability, appropriate selection of drugs, and good effect, but the process and management are complex and cause serious environmental pollution
At present, the hot oil wax removal vehicle widely used in oil fields has good wax removal effect, but this method has high operating costs and consumes a lot of fuel. In addition, water is used as the heat carrier during hot washing, which may also cause pollution to the formation.
In addition, too frequent wax removal operations also seriously affect the normal production of oil wells and bring great losses to the economic benefits of oil fields

Method used

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  • Novel microbial wax removal and control system and application thereof
  • Novel microbial wax removal and control system and application thereof
  • Novel microbial wax removal and control system and application thereof

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Effect test

preparation example Construction

[0023] 2. The preparation method of microbial wax-removing and anti-wax nutrient

[0024] At room temperature and normal pressure, add yeast extract with a mass ratio of 1 to 5%, dipotassium hydrogen phosphate 5 to 30%, ammonium nitrate 5 to 30%, and the balance with water, and stir at a low speed. Dissolve it completely in water for 4 hours, and distribute it in plastic buckets to make wax-removing and anti-wax nutrient. The wax-removing and anti-wax nutrient used in the present invention has fully considered the demand of bacterial strains for nutrients and the economics of the process in the well, including the requirements of carbon source, nitrogen source and nutrient salts. Under anaerobic conditions, high-efficiency electron acceptors are provided, thereby ensuring the biological activity of the Qingfangwax strain in the well (facultative anaerobic conditions). The concentration of the nutrient composition of the present invention has also been verified by many experim...

Embodiment 1

[0027] Embodiment 1: the preparation of expansion culture medium

[0028] First, remove the strains stored at -20°C with glycerol, streak and activate a single colony on the prepared solid broth medium plate, and culture it statically at 37°C for 3 days to obtain a single colony. Pick a single colony and inoculate it in a liquid broth medium, and cultivate it in a shaker at 37°C at 180rpm until it reaches the exponential growth phase as a strain. And according to the composition of the fermentation medium (g / L): peptone 1-5, ammonium salt 1-10, liquid paraffin 20-50, yeast powder 0.5-1.0, sodium chloride 5, pH 6-8 to prepare 100L fermentation medium, Insert 10L of 10% strains, cultivate to the stable stage, collect the bacterial liquid, and pack it in plastic barrels, store it at low temperature as the expanded culture liquid.

Embodiment 2

[0029] Embodiment 2: the preparation of microbiological wax-removing and anti-wax nutrient

[0030] Under the condition of room temperature and normal pressure, add yeast extract with a mass ratio of 5%, 30% dipotassium hydrogen phosphate, and 30% ammonium nitrate into the stirring tank in sequence, and the balance is mixed with clear water, and the nutrients are evenly dissolved in the stirring tank at a low speed. Water, divided into plastic barrels, and stored at low temperature.

[0031] In addition, the proportioning ratio of the microorganism wax-removing and anti-wax nutrient agent can also be: 1% yeast extract, 20% dipotassium hydrogen phosphate, 30% ammonium nitrate, and the balance is mixed with clear water.

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Abstract

The invention relates to a microbial wax removal and control system and an application method thereof. The invention is characterized by comprising the following steps of: preparing amplification culture solution of a Rhodococcus ruber Z25 bacterium; mixing the amplification culture solution with a wax removal and control nutrient agent to prepare a wax removal and control viscosity reducer; and injecting into an oil production shaft for wax removal and control treatment. The strain selected by the microbial wax removal and control system is high-activity hydrocarbon degrading flora, which can degrade paraffin wax in crude oil under the action of high-efficiency microbes, reduce the viscosity of the crude oil, improve the fluidity, remove the paraffin wax attached to the shaft, and effectively prolong the period of removing wax of an oil well; meanwhile, the wax removal and control nutrient agent can effectively ensure that the strain can effectively remove and control the wax under the shaft. The preparation cost for the system is low, the method is simple, and the system is convenient to apply, is suitable for large-scale promotion and application in high-wax oil wells and can effectively improve the economic benefit of oil field production.

Description

technical field [0001] The invention belongs to the technical field of wax removal and prevention in petroleum production, and in particular relates to a novel microbial wax removal and prevention system and its application. The microbial wax removal and prevention system involved can degrade paraffin in crude oil through the action of efficient microorganisms and reduce the viscosity of crude oil , improve fluidity, remove paraffin attached to the wellbore, effectively prolong the cycle of wax removal operations in oil wells, and improve economic benefits. Background technique [0002] During the crude oil extraction process, the wax in the crude oil will crystallize and precipitate in the wellbore due to the decrease of pressure and temperature, and adhere to the wellbore and pump valves, etc., affecting the normal operation of the liquid extraction system. Periodic wax removal is usually required The operation removes the wax attached to the wellbore. The wax removal and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K8/524C09K8/582E21B37/06E21B43/22
Inventor 郑承纲韩一凡张小霞温丹黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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