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Method for separating neutral longan polysaccharide fraction

A technology of longan polysaccharides and separation methods, applied in the field of separation of neutral longan polysaccharide fractions

Active Publication Date: 2011-05-25
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the separation and purification of neutral components of longan polysaccharides

Method used

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  • Method for separating neutral longan polysaccharide fraction
  • Method for separating neutral longan polysaccharide fraction
  • Method for separating neutral longan polysaccharide fraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select fresh longan raw materials, dry them, remove the skin and core, and crush them to obtain longan pulp; add 30 times the weight of water to the longan pulp, heat it to 80°C, and place it under 300W ultrasonic intensity for 30 minutes, cancel the ultrasonic and continue leaching Extract for 120 minutes; the extract is filtered and separated from the pulp residue, concentrated in vacuum at 60°C until slightly viscous, and the concentrated solution is collected and vacuum freeze-dried to obtain the longan pulp aqueous extract.

[0026] D301-F anion exchange resin is firstly soaked in water, poured out the suspended particles and impurities in the upper layer, then soaked in 4% NaCl solution by weight for 4 hours, and then washed with water; then soaked in 4% NaOH solution by weight for 4 hours , washed with water to a near-neutral pH value, and finally dried at 50°C for later use.

[0027] Dissolve the longan pulp extract in 20 times the weight of water, add 3 times t...

Embodiment 2

[0033]Select fresh longan raw materials, dry them, remove the skin and core, and crush them to obtain longan pulp; add 35 times the weight of water to the longan pulp, heat it to 75°C, and place it under 250W ultrasonic intensity for 40 minutes, cancel the ultrasonic and continue leaching Extract for 110 minutes; the extract is filtered and separated from the pulp residue, then concentrated in vacuum at 65°C until slightly viscous, and the concentrated solution is collected and vacuum freeze-dried to obtain the water extract of longan pulp.

[0034] D301-F anion exchange resin is firstly soaked in water, poured out the suspended particles and impurities in the upper layer, then soaked in 4.5% NaCl solution by weight for 3.5 hours, and then washed with water; then soaked in 4.5% by weight NaOH solution 3.5h, washed with water to near neutral pH value, and finally dried at 52.5°C for later use.

[0035] Dissolve the longan pulp extract in 25 times the weight of water, add 3.5 ti...

Embodiment 3

[0041] Select fresh longan raw materials, dry them, remove the skin and core, and crush them to obtain longan pulp; add 40 times the weight of water to the longan pulp, heat it to 70°C, and place it under 200W ultrasonic intensity for 50 minutes, cancel the ultrasonic and continue leaching Extracted for 100 min; the extract was filtered and separated from the pulp residue, concentrated in vacuum at 70°C until slightly viscous, and the concentrated solution was collected and vacuum freeze-dried to obtain the longan pulp aqueous extract.

[0042] D301-F anion exchange resin is firstly soaked in water, poured out the suspended particles and impurities in the upper layer, then soaked in 5% NaCl solution for 3 hours and washed with water; then soaked in 5% NaOH solution for 3 hours , washed with water to a near-neutral pH value, and finally dried at 55°C for later use.

[0043] Dissolve the longan pulp extract in 30 times the weight of water, add 4 times the D301-F anion exchange r...

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Abstract

The invention discloses a method for separating a neutral longan polysaccharide fraction. The method comprises the following steps of: (1) selecting longan fruit pulp water extract, and removing most of protein and pigment from the longan fruit pulp water extract through D301-F anion exchange resin to obtain crude longan polysaccharide; (2) separating and purifying the crude longan polysaccharide by a diethylaminoethyl DEAE-52 cellulose ion exchange chromatographic column, eluting with deionized water, collecting longan polysaccharide eluent, concentrating and freeze-drying under vacuum to obtain neutral longan polysaccharide powder; and (3) allowing the neutral longan polysaccharide to pass through a SephadexG-100 gel permeation chromatographic column, eluting with the deionized water, collecting different kinds of longan polysaccharide fraction eluent, concentrating and freeze-drying under vacuum to obtain the neutral longan polysaccharide fraction. By the method, a neutral single polysaccharide component can be separated and prepared.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a method for separating neutral longan polysaccharide fractions. Background technique [0002] Longan is mainly distributed in my country's Guangdong, Guangxi, Fujian and Taiwan, and is a typical subtropical economic crop. Longan is a medicinal and edible plant announced by the Ministry of Health. It is recorded in the "Compendium of Materia Medica" that "longan is the best source of benefits", and it is pointed out that longan meat has the effect of "appetizing and strengthening the spleen, nourishing deficiency and improving intelligence". And longan pulp is commonly used among the people to treat symptoms such as insufficiency of blood, heart palpitations, insomnia, forgetfulness, anemia, spleen deficiency and diarrhea. [0003] At present, the separation and preparation methods of longan polysaccharides are mainly water extraction and alco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张名位易阳魏振承唐小俊邓媛元张雁池建伟张瑞芬李健雄张业辉李武马永轩
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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