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Natural antioxidant polypeptide mixture and preparation method and application thereof

An anti-oxidation peptide and anti-oxidation peptide technology, applied in the biological field, can solve the problems of limited oxidation activity and high price, and achieve the effect of inhibiting the activity of melanin formation

Inactive Publication Date: 2012-07-04
梁醒财
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common antioxidants in cosmetics, such as vitamin C, vitamin E and SOD, etc., have limited oxidative activity
However, natural astaxanthin and other natural antioxidant products are generally too expensive due to their “natural” selling point, which is difficult to be accepted by the public.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] ① Acquisition of skin secretions: Stimulate the skin of the Yunnan stinky frog with a voltage of 6V for 6s, then wash the body of the stinky frog with 25mM Tris-HCl buffer (pH=7.8), collect the skin secretions, and put the collected secretions in Centrifuge at 4000rpm for 15min, vacuum freeze-dry the supernatant, and store at -80°C for later use;

[0020] ②Redissolve the lyophilized supernatant sample, and put it on a Sephadex G-75 gel filtration column equilibrated with 25mM Tris-HCl buffer (pH=7.8, containing 0.1M NaCl) in advance, at a flow rate of 0.3ml / min Carry out elution, collect the eluate every 10min; eluate is monitored by 280nm, draw the separation and purification map, collect the antioxidant active ingredients, and use C 4 The column removes the salt inside and then freezes and vacuum-dries to obtain a natural antioxidant peptide mixture.

[0021] The natural antioxidant polypeptide mixture is a gene-encoded product. Activity: 30% of free radicals can be ...

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Abstract

The invention relates to a natural antioxidant polypeptide mixture and preparation method and application of the mixture. The natural antioxidant polypeptide mixture is prepared from the skin of Odorrana frogs which can only be found in China and exits in frog skin to protect the exposed skin from ultraviolet injury. The antioxidant polypeptide mixture is applied to the preparation of cosmetics. The antioxidant polypeptide mixture is original and pure natural and conforms to the consumption needs of modern people, and the activity of the antioxidant polypeptide mixture is 1000 times higher than that of the conventional antioxidant butylated hydroxytoluene (BHT). Besides the antioxidant activity, the polypeptide mixture also has antimicrobial, immunoregulatory and melanin formation inhibiting activities.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a natural antioxidant polypeptide mixture and its preparation method and application in cosmetics. Background technique [0002] Free radicals are harmful compounds produced in the oxidation reaction of the body. They have strong oxidative properties and can damage the tissues and cells of the body, causing chronic diseases and aging effects. Under normal physiological conditions, the generation and removal of free radicals in the body are in a state of dynamic balance, but when the body is in an unfavorable environment and with age, the body will produce too many free radicals or remove them too slowly. Free radical scavengers, also known as antioxidants, can remove excess free radicals in the body and reduce their damage to the body. [0003] At present, it has been recognized that there are two sets of antioxidant systems in natural organisms. The first set is protein macromolecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61Q19/08G01N21/31
Inventor 梁醒财
Owner 梁醒财
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