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Natural antioxidant peptide Tiannanin-C1, and preparation method and application thereof

A technology of tiannanin-c1 and anti-oxidative peptides, which is applied in the preparation methods of peptides, chemical instruments and methods, cosmetic preparations, etc., can solve the problems of limited oxidation activity and high price

Inactive Publication Date: 2012-07-11
梁醒财
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common antioxidants in cosmetics, such as vitamin C, vitamin E and SOD, etc., have limited oxidative activity
However, natural astaxanthin and other natural antioxidant products are generally too expensive due to their “natural” selling point, which is difficult to be accepted by the public.

Method used

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  • Natural antioxidant peptide Tiannanin-C1, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0020] 1. Acquisition of skin secretions: Stimulate the skin of the Yunnan stinky frog with a voltage of 6V for 6s, then wash the body of the stinky frog with 25mM Tris-HCl buffer solution (pH=7.8), collect skin secretions, and collect the collected secretions Centrifuge at 4000rpm for 15min, vacuum freeze-dry the supernatant, and store at -80°C for later use;

[0021] 2. Purification of the polypeptide: redissolve the lyophilized supernatant sample, put it on a Sephadex G-75 gel filtration column equilibrated with 25mM Tris-HCl buffer (pH=7.8, containing 0.1M NaCl) in advance, and use 0.3 The flow rate of ml / min was eluted and collected every 10 min. The eluted product was monitored at 280nm, the separation and purification map was drawn, and the antioxidant active ingredients were collected, and used C 4 The column was freeze-dried after removing the salt inside. Afterwards, the sample was dissolved with 20 mM NaAc-HAc buffer (pH=4.0), purified with a Resource S separation...

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Abstract

The invention discloses natural antioxidant peptide Tiannanin-C1, and a preparation method and application thereof. The natural antioxidant peptide Tiannanin-C1 is a gene coding product, and the sequence from a terminal N to a terminal C is RLFKCYKPDS. The natural antioxidant peptide Tiannanin-C1 is prepared from specific Odorrana graminea Boulenger in China; the antioxidant peptide Tiannanin-C1 in Batrachia skin has the main function of protecting exposed skin from being hurt by ultraviolet, and the natural antioxidant peptide Tiannanin-C1 is applied to preparation of cosmetics. The natural antioxidant peptide Tiannanin-C1 returns to the source and is purely natural, and meets the current consumption requirement of masses. The activity of the natural antioxidant peptide Tiannanin-C1 is 1,000 times that of the conventional antioxidant butylated hydroxytoluene (BHT). The natural antioxidant peptide Tiannanin-C1 has antioxidant activity, certain antibacterial activity, immune adjustment activity and melanin inhibiting activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a natural antioxidant peptide Tiannanin-C1 with antioxidant activity, its preparation method and its application in cosmetics. Background technique [0002] Free radicals are harmful compounds produced in the oxidation reaction of the body. They have strong oxidative properties and can damage the tissues and cells of the body, causing chronic diseases and aging effects. Under normal physiological conditions, the generation and removal of free radicals in the body are in a state of dynamic balance, but when the body is in an unfavorable environment and with age, the body will produce too many free radicals or remove them too slowly. Free radical scavengers, also known as antioxidants, can remove excess free radicals in the body and reduce their damage to the body. [0003] At present, it has been recognized that there are two sets of antioxidant systems in natural organisms. The firs...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/20C07K1/16A61K8/64A61Q19/08
Inventor 梁醒财
Owner 梁醒财
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