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Selenylation honeylocust kernel polysaccharide gum and preparation method thereof

The technology of acacia kernel and polysaccharide gum is applied in the field of selenized polysaccharide gum and its preparation, which can solve problems such as harsh reaction conditions, and achieve the effects of low cost, reduced toxicity and abundant raw materials

Inactive Publication Date: 2011-05-18
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Invention patent 200910162003.4 uses N,N-dimethylformamide as solvent, reacts with selenous acid and thionyl chloride to prepare selenized fern polysaccharides and artemisia polysaccharides, but the reaction conditions are relatively harsh
Research papers (Zhang Ji et al., "Polymer Bulletin", No. 10, 2009) reported that under the catalysis of nitric acid and barium chloride, sodium selenite was used to prepare polysaccharide selenate from Lanzhou lily, with a selenium content of 784 μg / g, but the lily polysaccharide involved in this technology is a water-soluble polysaccharide and does not involve polysaccharide gum

Method used

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  • Selenylation honeylocust kernel polysaccharide gum and preparation method thereof
  • Selenylation honeylocust kernel polysaccharide gum and preparation method thereof
  • Selenylation honeylocust kernel polysaccharide gum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Extraction of saponins polysaccharide gum:

[0026] Weigh 5g of dried acacia kernels, soak in distilled water overnight, add 500mL of distilled water, ultrasonic for 9min, extract at 85°C for 2 hours, centrifuge (speed 4000r / min) for 30min, collect the clear liquid, add 500mL of distilled water to the precipitate, ultrasonic for 9min, 85 Extract at ℃ for 2 hours, centrifuge (rotating speed 4000r / min) for 30min, and repeat the extraction 5 times. The combined supernatant was concentrated by rotary evaporation to 1 / 4 of the original volume, and a volume concentration of 95% ethanol with a volume ratio of 1:4 was added, and a white flocculent precipitate appeared, which was separated by centrifugation (rotating speed 4000r / min), and the precipitate was separated with ethanol, acetone, and ether. Washing, freeze-drying to obtain acacia kernel polysaccharide gum as a white solid powder.

Embodiment 2

[0028] Weigh 1g of dried saponins polysaccharide gum, add 100mL weight concentration of 1.5% HNO 3 Solution, ultrasonic 10min, stirred at 80°C for 1h, dissolved, added 1.5gBaCl 2 React with 0.85g sodium selenite at 80°C for 10h, add Na 2 SO 4 There is white precipitate, centrifuge (3000r / min) to remove the precipitate, and dialyze the clear liquid with distilled water. During this period, take a small amount of dialysate and add Vc to test. After 5 days, no red appears, and the conductivity drops to 15.5μS.cm -1 , stop dialysis. Suction filter the dialysate, add 95% ethanol to the clear liquid at a volume ratio of 1:4, a large amount of white flocculent precipitates appear, stand overnight, centrifuge (3000r / min) to separate, and wash the precipitates with absolute ethanol, acetone, and ether respectively Twice, vacuum drying at 70°C (vacuum degree 0.4MPa) to obtain a white solid powder. Its chemical structure is shown in formula (1).

[0029] Weigh 10mg of saponins jelly...

Embodiment 3

[0033] The scavenging effect of embodiment 3 saponins japonicus kernel polysaccharide gum to hydroxyl free radical:

[0034] Acacia japonicum polysaccharide gum is insoluble in water, but selenized acacia japonica jelly polysaccharide gum is easily soluble in water, so the scavenging effect of selenized acacia japonica jelly polysaccharide gum aqueous solution on hydroxyl free radicals was determined.

[0035] Draw 9mmol·L respectively -1 FeSO 4 ·7H 2 O solution, 9mmol·L -1 Salicylic acid-ethanol solution, 8.8mmol·L -1 H 2 o 2 Add 1 mL each of the solution and deionized water to a cuvette. After stirring slightly, place it in a dark place for 30 minutes. Measure the absorbance at 510nm with a UV spectrophotometer, and the obtained data is A 0 .

[0036] Absorb 9mmol·L respectively -1 FeSO 4 ·7H 2 O solution, 9mmol·L -1 1 mL of salicylic acid-ethanol solution and 1 mL of selenized saponins polysaccharide gum aqueous solution in a cuvette, and finally 1 mL of deioni...

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Abstract

The invention provides a selenylation honeylocust kernel polysaccharide gum and a preparation method thereof. The preparation method comprises the following steps of: (1) adding honeylocust kernel polysaccharide gum into HNO3 solution, and dispersing; and (2) adding BaCl2 and sodium selenite for reaction, dripping Na2SO4, settling, collecting supernate, dialyzing the supernate by using water, collecting clear liquid, adding 90 to 95 percent ethanol into the clear liquid in a volume ratio of 1:2-6, settling, and collecting the sediment, namely the selenylation honeylocust kernel polysaccharide gum. The selenylation honeylocust kernel polysaccharide gum is formed by introducing selenium into a saccharide unit of the honeylocust kernel polysaccharide gum, the toxicity of selenious acid groups can be effectively reduced, selenylation honeylocust kernel polysaccharide easily dissolved in water is obtained, and the selenylation honeylocust kernel polysaccharide gum has clearing effect on free hydroxyl radicals. The selenylation honeylocust kernel polysaccharide gum has the following molecular formula.

Description

technical field [0001] The invention relates to a selenized polysaccharide gum and a preparation method thereof. Background technique: [0002] Selenium is an essential trace element for life. Studies have found that selenium can delay aging, prevent cancer and cardiovascular diseases, and treat Keshan disease and Kashin-Beck disease. Selenium enhancers in my country include inorganic selenium and organic selenium. Inorganic selenium enhancers mainly include sodium selenite and sodium selenate; organic selenium enhancers mainly include selenoprotein, selenium-enriched yeast, selenized carrageenan, selenium polysaccharide, Although selenium amino acids, selenium nucleic acids, etc., inorganic selenium fortifiers have high selenium content and low price, their absorption is not ideal, their bioavailability is low, and their toxicity is high, so their usage is strictly limited. The organic selenium enhancer has high safety, is not easy to be poisoned, is easily absorbed and uti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
Inventor 陈燕青张晓露唐博合金徐菁利郑静
Owner SHANGHAI UNIV OF ENG SCI
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