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Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage

A feed additive and compound technology, applied in animal feed, animal feed, anti-fungal/algae/lichen immunoglobulin, etc., can solve the problems of easy secondary infection, easy inactivation, antibiotic residue, etc., to improve immunity Disease resistance and production efficiency, radiation-resistant animal performance, effects of improving animal performance

Active Publication Date: 2005-03-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects that the feed additives in the prior art are easy to cause antibiotic residues, poor thermal stability, easy inactivation, large addition ratio, high cost, and some preparations still have toxic and side effects, especially to overcome some antibiotics that cause animal The shortcomings of obvious suppression of immune function, decreased disease resistance, and easy to cause secondary infection, thus providing a non-toxic, harmless, no residue, safe, high-efficiency, low-cost, can significantly promote the development of animal immune system, enhance animal immunity , a glycopeptide complex immunoenhancing feed additive that improves disease resistance

Method used

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  • Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, prepare the fermented liquid L-1 of Ganoderma lucidum

[0035] 1) slant culture activation: in a 15ml test tube, inoculate the mycelium of Ganoderma lucidum on the slant medium of modified potato dextrose agar (PGA), and cultivate it for 4 days at 25°C until the mycelium covers the slant; The specific preparation method of the slant medium of the improved potato dextrose agar (PGA) is: 200 grams of potatoes are peeled and cut into small slices or small pieces, added to 500 ml of boiling water and boiled for 30 minutes, then filtered with 4 layers of gauze, in the filtered juice Add 20 grams of glucose and 2 grams of yeast powder, then add water to make up to 1000ml, subpackage, sterilize, and set aside;

[0036] 2) Prepare shake flask seed liquid: put the agar slant medium covered with mycelia obtained in step 1) into about 100ml of liquid medium A, and cultivate it on a shaker at 25°C with a rotation speed of 80 rpm for 2 days. Obtain the shake flask see...

Embodiment 2

[0041] Embodiment 2, prepare the fermented liquid L-II of Ganoderma lucidum

[0042] 1) slant culture activation: in a 15ml test tube, inoculate the mycelium of Ganoderma lucidum on the slant medium of modified potato dextrose agar (PGA), and cultivate it for 7 days at 27°C until the mycelium covers the slant; The specific preparation method of the slant medium of the improved potato dextrose agar (PGA) is: 250 grams of potatoes are peeled and cut into small slices or small pieces, added to 500 ml of boiling water and boiled for 30 minutes, then filtered with 4 layers of gauze, in the filtered juice Add 20 grams of glucose and 2 grams of yeast powder, then add water to make up to 1000ml, subpackage, sterilize, and set aside;

[0043] 2) Prepare shake flask seed liquid: put the agar slant medium covered with mycelium obtained in step 1) into about 100ml liquid medium A, and cultivate it on a shaker at 27° C. with a rotation speed of 120 rpm for 3 days. Obtain the shake flask s...

Embodiment 3

[0047] Embodiment 3, prepare the fermented liquid L-III of Ganoderma lucidum

[0048] 1) slant culture activation: in a 15ml test tube, the mycelium of Ganoderma lucidum was inoculated on the slant medium of modified potato dextrose agar (PGA), and cultivated at 32°C for 10 days until the mycelium covered the slant; The specific preparation method of the slant medium of the improved potato dextrose agar (PGA) is: 300 grams of potatoes are peeled and cut into small slices or small pieces, added to 500 ml of boiling water and boiled for 30 minutes, then filtered with 4 layers of gauze, in the filtered juice Add 20 grams of glucose and 2 grams of yeast powder, then add water to make up to 1000ml, subpackage, sterilize, and set aside;

[0049] 2) Prepare shake flask seed liquid: put the agar slant medium covered with mycelium obtained in step 1) into about 100ml liquid medium A, and cultivate it on a shaker at 35° C. with a rotation speed of 200 rpm for 5 days. Obtain the shake f...

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Abstract

The invention relates to an immunity strengthened feedstuff additive of a glycopeptide composite, a main component of the additive is a fermentation liquor of glossy ganoderma or / and coriolus versicolor. The fermentation liquor is obtained by inoculating a mycelium the glossy ganoderma or / and coriolus versicolor on a slope culture medium of an improved potato glucose agar; then adding a liquid culture medium A to prepare a shake flask seed liquor; subsequently adding a liquid culture medium B to prepare a first level fermentation seed liquor; finally adding a liquid culture medium C and deep liquid fermentating. The fermentation liquor is further processed to obtain a glycopeptide composite immunity strengthened feedstuff additive PSPc-1 (crystal elaboration product), PSPc-2 (adsorbent powder), PSPc-3 (liquid agent) and PSPc-4 (mycelium powder) of the invention respectively. The feedstuff additive would not generate residue of antibiotics, is able to promote animal growing or improve production efficiency, stimulate growth of immune systems, enhance immunity and disease resistance, and the feedstuff additive has a goode heat stability, is difficult to dead, is an innocuous, no-residue, safety, high efficiency, low cost immunity strengthened feedstuff additive of the glycopeptide composite.

Description

technical field [0001] The invention relates to a feed additive and a preparation method thereof, in particular to a glycopeptide complex immunoenhancing feed additive produced by fermenting Ganoderma lucidum and Yunzhi, as well as a preparation method and application thereof. Background technique [0002] Feed additives play an indispensable role in promoting growth, preventing diseases and improving production efficiency in livestock and poultry farming. Early feed additives were mainly antibiotics, which played an important role in the prevention of animal diseases and growth promotion, but scientific research also found that the drug residues of antibiotics endanger the health of the public and have a great impact on the export of animal products in my country. negative impact. With the improvement of people's food safety awareness, it is urgent to use green feed additives to produce green animal products, pollution-free food, antibiotic-free food or safe food. [0003]...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/30A23K20/147A23K20/24C07K16/14
Inventor 张日俊王铮
Owner CHINA AGRI UNIV
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