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Application of toyocamycin in prevention and control of cucumber rhizoctonia rot

A technology of toyocamycin and blight, applied in the field of microorganisms, can solve problems such as the types of unsafe microbial source pesticides

Inactive Publication Date: 2011-04-27
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of actinomycete-amylase chromogenous Streptomyces D isolated and screened out from Hangzhou soil for the deficiency that chemical pesticide is unsafe and microbial source pesticide kind is less; Another purpose of the present invention is Provides a microbial pesticide for preventing and treating some plant fungal diseases by using the strain to extract its metabolites through fermentation and a preparation method thereof

Method used

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  • Application of toyocamycin in prevention and control of cucumber rhizoctonia rot
  • Application of toyocamycin in prevention and control of cucumber rhizoctonia rot
  • Application of toyocamycin in prevention and control of cucumber rhizoctonia rot

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Embodiment 1

[0021] Embodiment 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)

[0022] The present invention obtained the amylase chromogenic Streptomyces chromogen from the soil sample collected in Tianmu Mountain, Lin'an, Zhejiang Province in August 2006, through the steps of separation, cultivation, fermentation, activity determination and screening of effective strains for inhibiting plant fungal disease pathogens. diastatochromogenes) D, preservation.

[0023] Determination of antibacterial activity of amylase Streptomyces diastatochromogenes D: Take 1mL of its fermentation supernatant in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and mix it evenly with 9mL of PDA medium cooled to 50°C. Place a 4mm-diameter Cucumber solanum blight bacteria cake for testing on the base plane, connect the cake to the center of the petri dish (the diameter of the petri dish is 9cm), put it in an incubator for 36 hours at 28°C, and treat it with sterile water a...

Embodiment 2

[0026] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolite)

[0027] Follow these steps:

[0028] (1) Bacteria test tube slant activation culture: the slant medium is Gaoshi No. 1 medium, and its components and content are: K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, 20g soluble starch, 0.5g NaCl, FeSO 4 0.01g, 20g of agar, add water to 1L after dissolving; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15×150mm ), placed in an incubator at 28°C±1°C, activated for 96 hours, until the slant of the test tube is covered with spores;

[0029] (2) Fermentation culture: The fermentation medium contains 21.5g of yellow corn flour, 10g of soluble starch, 5g of bran, and CaCO per 1000mL. 3 2.5g, NH 4 NO 3 1.5g, FeSO 4 0.5g, KH 2 PO 4 1.5g, the balance is water; 40mL fermentation broth per 300mL triangu...

Embodiment 3

[0031] Embodiment 3: (the biocontrol effect of toyocamycin to cucumber blight)

[0032] Follow these steps:

[0033] The pot experiment method was used to determine the biocontrol effect of toyocamycin on cucumber blight. The indoor potting of cucumber seedlings was carried out in a plastic pot with a height of 11 cm and an upper diameter of 14 cm. When the melon seedlings grow to the four-leaf stage, select plants with consistent growth for the test. Rhizoctonia solani of cucumber was inoculated by root irrigation method. The toyocamycin activity test included 2 treatments of toyocamycin mass concentration of 19.5mg / L and 9.8mg / L, each treatment was repeated 3 times, and 98% hymexazol wettable powder was used as the drug control, and water was set For blank control.

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Abstract

The invention discloses an application of toyocamycin in prevention and control of cucumber rhizoctonia rot, which belongs to the technical field of microorganism. The method comprises the following steps: performing strain activation culture on streptomyces diastatochromogenes separated and screened from the soil samples collected from Tianmu Mountain, Lin'an, Hangzhou, Zhejiang; performing fermentation culture; extracting the fermented product; and separating and purifying the fermented product to obtain the active compound toyocamycin. Pot experiments indicate that the toyocamycin has obvious treatment and protection effect on cucumber rhizoctonia rot and better prevention and control effect than the conventional chemical agents, while ensuring safety.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the cultivation of biocontrol actinomycete-amylase Streptomyces diastatochromogenes D and the separation and application of metabolites. Background technique [0002] Actinomycetes are closely related to humans. According to statistics, 70% of the discovered antibiotics are produced by actinomycetes. Soil is an important place for actinomycetes to inhabit. Obtaining actinomycetes with high antibacterial activity from soil to produce antibiotics is an indispensable basic work for the development of new medicines and new pesticides worldwide. [0003] At present, the control of plant diseases mainly adopts the method of chemical pesticide control. Although chemical pesticides have ideal control effects on many diseases, long-term use will induce the resistance of bacteria. In addition, the frequent use of pesticides not only increases the production cost of agricultural product...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00C12P19/32C12R1/465
Inventor 申屠旭萍俞晓平于冰郝培应董胜张边亚琳
Owner CHINA JILIANG UNIV
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