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Bacillus licheniformis bacterial strain and application thereof

A technology of Bacillus licheniformis and bacterial strains, which is applied to novel Bacillus licheniformis strains and its application field in the production of crops, can solve problems such as unseen technical reports, biological strains or biological inoculum blanks, etc., and achieves overcoming soil acidification, Improves resistance and promotes growth

Active Publication Date: 2011-01-26
内蒙古四季丰盈农牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the technology of biological strains or biological agents applied to the planting of yellow bark or other plants is still blank, and no technical reports have been seen.

Method used

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  • Bacillus licheniformis bacterial strain and application thereof
  • Bacillus licheniformis bacterial strain and application thereof
  • Bacillus licheniformis bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Wash the fresh samples of the roots of the yellow-skinned plants in Panyu soil with distilled water, then cut the roots and place them in three sterilized petri dishes, and wash them with 3% hydrogen peroxide (H 2 o 2 ) soaked for 4 minutes to remove the rotten substances on the root surface, washed once with sterile water, and then used 0.1% mercuric chloride (HgCl 2 ) soaked for 5 minutes, and washed with vibration in sterile water for 5 times, each time for 8 minutes. The completely surface-sterilized roots were chopped up with a sterilized scalpel, and inoculated into three test tubes containing 5ml of Nfb semi-solid medium respectively. After sealing with rubber stoppers, they were placed in an artificial incubator at 28°C for cultivation. The whole operation is completed under sterile conditions. After the bacterium grows, inject 1 / 10 volume of 10% acetylene gas (final concentration: 1%) into the tube, and cultivate it for another 24 hours under the same conditi...

Embodiment 2

[0056] Application experiment of embodiment 2 vegetable planting aspect

[0057] The single bacterium colony obtained by activation adopts LB liquid culture based on 28 ℃ and cultivates for 48 hours, and mixes with vermiculite with 1: 2 weight ratio (1 liter of culture fluid mixes 2 kilograms of vermiculites) to make novel bacillus licheniformis-vermiculite inoculant. LB liquid medium composition: Yeast extact (yeast extract) 5g, Peptone (peptone) 10g, NaCl (sodium chloride) 10g, deionized water 1000mL.

[0058] Adjust the pH value of the soil to 5.0, 6.0, and 7.0 in advance, and apply the Bacillus licheniformis-vermiculite inoculant to the lettuce seedlings for 15 days. Those skilled in the art will determine the application dosage according to the specific needs. In this embodiment, each 10 One kilogram of soil was mixed with 10g of vermiculite inoculant and applied. After 75 days, compared with the uninoculated control (according to every 10 kg of soil mixed with 10 g of v...

Embodiment 3

[0059] Embodiment 3 Applied experiments on the aspect of yellow skin planting

[0060] The single bacterium colony obtained by activation is cultivated by LB liquid culture based on 28 ℃ for 48 hours, and mixed with the commercially available rice bran as the substrate bio-fertilizer at a weight ratio of 1:20 (1 liter of culture solution mixed with 20 kg of rice bran-based bio-fertilizer) Made into Bacillus licheniformis-biological fertilizer. The bacillus licheniformis-biological fertilizer was applied to the wampum plants in the growth stage, and the routinely applied chemical fertilizer group was used as control. According to the rhizosphere of each yellow bark tree, apply 1 kg of biological fertilizer to inoculate the yellow bark of the bacterial fertilizer, the fruit is full, the size is uniform, and the leaf color is dark green, see attached image 3 As shown in the left figure; the non-inoculated control group (applying 1 kilogram of bran substrate according to the rhi...

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Abstract

The invention discloses a bacillus licheniformis bacterial strain and application thereof. The bacterial strain 1*2-4 was collected in General Microbiology Centre of China Committee for Culture Collection of Microorganisms on June 4th, 2010, wherein the collection number is CGMCC No.3911. The bacterial strain of the invention has the characteristics of nitrogen fixation and high temperature resistance. The invention also provides a separation and purification method for the bacterial strain and application of the bacterial strain in preparing a microbial inoculum or a biofertilizer for promoting plant growth and improving disease resistance, can effectively promote the growth and the disease resistance of clausena lansium, lettuces and other crops.

Description

technical field [0001] The invention belongs to the field of biological fertilizers, and relates to a novel bacillus licheniformis strain and its application in the production of crops. Background technique [0002] Bacillus bacteria are a type of aerobic or facultative anaerobic rod-shaped bacteria that produce stress-resistant endospores. They live in saprophytes and are widely distributed in nature, mainly distributed in soil, plant surfaces and water bodies, and grow quickly. , Simple nutrition, easy to separate and cultivate, strong stress resistance, heat resistance, dry resistance, UV resistance, electromagnetic radiation resistance, fast propagation speed, easy to colonize on the plant surface. As an important microbial resource, Bacillus is rich in enzymes such as protease, amylase, lipase and other abundant metabolites. Some bacteria can also decompose insoluble phosphate in the soil, and play a role in the soil together with other microorganisms. play an importan...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00A01P1/00A01P3/00C05F11/08C12R1/10
CPCY02W30/40
Inventor 蒙志洪
Owner 内蒙古四季丰盈农牧业科技有限公司
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