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Heat-resisting and freeze-drying protector for live vaccine and preparation method thereof as well as live newcastle disease vaccine

A technology of heat-resistant freeze-drying and Newcastle disease, which is applied to medical preparations, antiviral agents, and pharmaceutical formulas with non-active ingredients, and can solve the problems of long-term storage and long-distance transportation restrictions, harsh storage conditions, and poor protective performance of vaccines. Achieve the effect of long storage time, stable protection performance and good protection effect

Inactive Publication Date: 2011-01-19
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the protective agents commonly used by domestic vaccine manufacturers are mainly sucrose, milk, gelatin, etc., with simple formulations and poor protective performance
If it is stored at 2-8°C, the storage period is only 3-6 months, and it is often necessary to store it below -15°C. The storage conditions are relatively harsh, which greatly restricts the long-term storage and long-distance transportation of the vaccine. In the specific use process It is easy to cause immune failure and cause epidemics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] 1. Preparation of Live Newcastle Disease Vaccine

[0018] Measure the hemagglutination titer of the chicken Newcastle disease vaccine strain to be 640, dilute it 20 times with sterile normal saline, and insert 0.2ml of the dilution solution of the Newcastle disease vaccine strain into 9-10-day-old SPF chicken embryos under aseptic conditions, and each chicken Embryos were inoculated in the allantoic cavity, sealed with paraffin and incubated in a 36°C incubator for 4-5 days. After the culture was completed, they were placed in a 4°C refrigerator overnight, and then the allantoic fluid was taken out under aseptic conditions. Finally, the hemagglutination titer of the virus was determined to be between 604-1280, and then a 0.45 μ microporous filter membrane was used to filter under pressure.

[0019] 2. Configuration of heat-resistant freeze-drying protective agent

[0020] The protective agent can be divided into two parts: one part is high temperature resistant polyvin...

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Abstract

The invention discloses a heat-resisting and freeze-drying protector for a live vaccine and a preparation method thereof as well as a live newcastle disease vaccine adopting the freeze-drying protector. The heat-resisting and freeze-drying protector for the live vaccine comprises the following compositions in percentage by weight: 10-15 percent of trehalose, 1-2 percent of polyvinylpyrrolidone (PVP), 1-2 percent of alum, 1-2 percent of glutamine, 2-10 percent of sorbitol, 0.1-0.5 percent of potassium dihydrogen phosphate, 0.5-1 percent of vitamin C and the balance of water. Compared with the traditional vaccine, the heat-resisting and freeze-drying protector for the live vaccine has the advantages of good stability, long preservation time, low preservation condition, little activity damage on the live vaccine, good dissolubility, simple raw material and the like.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant of chicken Newcastle disease live vaccine, a preparation method of the heat-resistant freeze-dry protectant, and a chicken Newcastle disease live vaccine using the heat-resistant freeze-dry protectant. Background technique [0002] Newcastle disease (ND), also known as Asian chicken plague or pseudo chicken plague, is an acute, highly contagious and fatal infectious disease of poultry caused by Newcastle disease virus (NDV), which is widely prevalent in many countries in the world. It is characterized by dyspnea, diarrhea, nervous disorders, mucosal and serosal hemorrhage, which seriously endangers the poultry industry in the world and is listed as a class A infectious disease by the World Health Organization (OIE). [0003] NDV is a single-strand, negative-strand, non-segmented RNA virus. It is an approximately spherical particle with an envelope, generally 100-400nm in diameter. The v...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/32A61K39/17C08L5/04C08L39/06C08K13/02A61P31/14
Inventor 吴红云李建正范金红
Owner 河南后羿生物工程股份有限公司
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