Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting diazepam and chemoluminescence immunoassay kit special for same

A technology of chemiluminescence immunity and diazepam, which can be applied in microorganism-based methods, biological tests, immunoglobulins, etc., can solve problems such as affecting physical health, drug residues, life-threatening safety, etc., and achieves good preservation and stability. , Easy operation, high sensitivity effect

Active Publication Date: 2011-01-05
CHINA AGRI UNIV
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abuse of diazepam in feed will eventually lead to drug residues in animal products, which will seriously affect people's health and threaten people's lives

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting diazepam and chemoluminescence immunoassay kit special for same
  • Method for detecting diazepam and chemoluminescence immunoassay kit special for same
  • Method for detecting diazepam and chemoluminescence immunoassay kit special for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, preparation and detection method of chemiluminescent immunoassay kit

[0037] 1. The composition of the chemiluminescence immunoassay kit:

[0038] (1) Original coating solution: obtained by dissolving the original coating in the coating buffer, wherein the concentration of the original coating is 0.08 μg / mL; the original coating is the coupling of diazepam hapten and ovalbumin thing.

[0039] (2) Horseradish peroxidase-labeled goat anti-mouse anti-antibody working solution: obtained by diluting horseradish peroxidase-labeled goat anti-mouse anti-antibody with diluent, the dilution ratio is 1:1000; the diluent consists of 50mL It is obtained by mixing bovine serum albumin and 950 mL of phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH value is 7.4. Horseradish peroxidase-labeled goat anti-mouse anti-antibody was purchased from Jackson ImmunoResearch Laboratories Inc. catalog number 115-035-003.

[0040] (3) Diazepam standa...

Embodiment 2

[0086] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0087] 1. Kit sensitivity experiment

[0088] The zero standard solution (that is, the diluent is pH7.4, 0.05M phosphate buffer) was tested 20 times, and the average value of the measurement results plus 3 times the standard deviation was used as the minimum detection limit of the kit.

[0089] Table 1 Statistical table of zero standard measurement results μg / L

[0090]

[0091] It can be seen from Table 1 that the minimum detection limit of the kit is 0.1 μg / L.

[0092] 2. Standard product precision test:

[0093] From the described three batches of kits (01 batches, 02 batches, 03 batches) obtained by Example 1, 10 kits are extracted from each batch, and the measurement concentration is the luminescence intensity of the diazepam standard solution of 1 μg / L value, and calculate the coefficient of variation. The detection method is consistent with that described in Experiment 3 in Example 1.

[009...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting diazepam and a chemoluminescence immunoassay kit special for the same. The chemoluminescence immunoassay kit special for detecting the diazepam provided by the invention comprises a diazepam specific antibody, a coating antigen and standard substance solution, wherein the coating antigen is a conjugate of a diazepam semi-antigen and a carrier protein. The detection method of the invention has the characteristics of simple sample pretreatment process, simple and convenient operation, low cost, high specificity, high sensitivity, high accuracy and the like, can be monitored on site and is suitable for screening a large number of samples.

Description

technical field [0001] The invention relates to a method for detecting diazepam and a special chemiluminescence immunoassay kit thereof. Background technique [0002] At present, the safety of animal food has become the focus of attention all over the world, and the problem of veterinary drug residues is one of the most important factors affecting the safety of animal food. Due to the complex composition of animal samples, the concentration of analytes is low, and most of the samples are very small , which puts forward higher requirements on the selectivity and sensitivity of the analytical method. Chemiluminescence immunoassay (Chemiluminescence analysis, CLIA) technology combines highly sensitive chemiluminescence technology with highly specific immune reaction, which has high sensitivity, strong specificity, wide linear range, easy operation, and does not require very expensive equipment Features. CLIA does not require external light sources, has a higher signal-to-nois...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53G01N33/577G01N33/531C07K16/44C12N5/20C12R1/91
Inventor 史为民沈建忠张素霞王战辉吴聪明程林丽曹兴元汤树生
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com