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Method for detecting ractopamine and chemiluminescence immunoassay kit special for same

A chemiluminescence immunity, ractopamine technology, applied in chemiluminescence/bioluminescence, microorganism-based methods, immunoglobulins, etc., can solve the problems of ractopamine toxicity, public health, tachycardia, etc. The effect of good performance and stability, low cost and high accuracy

Inactive Publication Date: 2010-12-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ractopamine has relatively high toxicity and side effects. When the cumulative intake exceeds a certain value, it will cause poisoning symptoms such as muscle tremors, tachycardia, and arrhythmia in humans and animals. Countries such as the European Union and China prohibit its use as a feed additive. However, there are often illegal applications in livestock production, resulting in residues in livestock and poultry products, endangering public health

Method used

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  • Method for detecting ractopamine and chemiluminescence immunoassay kit special for same
  • Method for detecting ractopamine and chemiluminescence immunoassay kit special for same
  • Method for detecting ractopamine and chemiluminescence immunoassay kit special for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, preparation and detection method of chemiluminescent immunoassay kit

[0041] 1. The chemiluminescent immunoassay kit includes:

[0042] (1) It is obtained by dissolving the coating original in the coating buffer, wherein the concentration of the coating original in the coating original solution is 0.08 μg / mL; the coating original is the coupling of ractopamine hapten and ovalbumin things.

[0043] (2) Horseradish peroxidase-labeled goat anti-mouse anti-antibody working solution: obtained by diluting horseradish peroxidase-labeled goat anti-mouse anti-antibody with diluent, the dilution ratio is 1:1000;

[0044] The diluent is obtained by mixing 50mL bovine serum albumin and 950mL phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH value is 7.4.

[0045] Horseradish peroxidase-labeled goat anti-mouse anti-antibody was purchased from Jackson ImmunoResearch Laboratories Inc. catalog number 115-035-003.

[0046](3) Ractopamine ...

Embodiment 2

[0092] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0093] 1. Kit sensitivity experiment

[0094] The zero standard solution (that is, the diluent is pH7.4, 0.05M phosphate buffer) was tested 20 times, and the average value of the measurement results plus 3 times the standard deviation was used as the minimum detection limit of the kit.

[0095] Table 1 Statistical table of zero standard measurement results μg / L

[0096]

[0097] It can be seen from Table 1 that the minimum detection limit of the kit is 0.1 μg / L.

[0098] 2. Standard product precision test:

[0099] From the three batches of kits described in Example 1 (batch 01, batch 02, and batch 03), 10 kits were extracted from each batch, and the luminous intensity value of the 1 μg / L standard solution was measured to calculate the coefficient of variation. The detection method is consistent with that described in Experiment 3 in Example 1.

[0100] The experiment was repeated three times, and...

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Abstract

The invention discloses a method for detecting ractopamine and a chemiluminescence immunoassay kit special for the same. The chemiluminescence immunoassay kit for detecting the ractopamine provided by the invention comprises a ractopamine specific antibody, a coating antigen and standard solution, wherein the coating antigen is a conjugate of ractopamine hapten and carrier protein. The method for detecting the ractopamine has the characteristics of simple pre-processing process of a sample, simple and convenient operation, low cost, high specificity, high flexibility, high accuracy and the like, on-site monitoring can be preformed by the method and the method is suitable for screening of a great number of samples.

Description

technical field [0001] The invention relates to a method for detecting ractopamine and a special chemiluminescent immunoassay kit thereof. Background technique [0002] At present, the safety of animal food has become the focus of attention all over the world, and the problem of veterinary drug residues is one of the most important factors affecting the safety of animal food. Due to the complex composition of animal samples, the concentration of analytes is low, and most of the samples are very small , which puts forward higher requirements on the selectivity and sensitivity of the analytical method. Chemiluminescence immunoassay (Chemiluminescence analysis, CLIA) technology combines highly sensitive chemiluminescence technology with highly specific immune reaction, which has high sensitivity, strong specificity, wide linear range, easy operation, and does not require very expensive equipment Features. CLIA does not require external light sources, has a higher signal-to-no...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531G01N21/76C07K16/44C12N5/20C12R1/91
Inventor 王战辉沈建忠张素霞史为民吴聪明程林丽曹兴元汤树生
Owner CHINA AGRI UNIV
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