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Canine distemper attenuated vaccine strain and application thereof

A technology of attenuated vaccine and canine distemper virus, which is applied in the field of canine distemper prevention and control, can solve the problems of high cost and low antigenicity, and achieve good effect, good immune protection and long-lasting immunity

Inactive Publication Date: 2010-12-15
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the above vaccines have disadvantages such as high cost and poor antigen specificity. Therefore, it is imminent to develop a new and efficient CD vaccine.

Method used

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  • Canine distemper attenuated vaccine strain and application thereof
  • Canine distemper attenuated vaccine strain and application thereof
  • Canine distemper attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation and identification of canine distemper virus (CDV-YB) isolates

[0016] 1.1 Test material

[0017] 1.1.1 Disease data

[0018] The affected dog came from the Yanbian area of ​​Jilin Province. The clinical manifestations were elevated body temperature and purulent discharge from the eyes and nose. The sick dog failed to be treated with a large amount of antibiotics.

[0019] 1.1.2 Cells, nutrient solutions and reagents

[0020] Chicken embryo fibroblasts were prepared by our laboratory. The cell growth medium and maintenance medium were MEM nutrient solution containing 10% and 2% calf serum and serum-free DMEM respectively; FITC-labeled goat anti-rabbit secondary antibody and gel recovery reagent Boxes were purchased from Bao Biological Engineering Co., Ltd. CDV A75-17 and 5804 strains were purchased from ATCC.

[0021] 1.1.3 Test animals

[0022] 2-4 months old healthy puppies (CDV antibody titer ≤1:2).

[0023] 1.2 Method

[0024] 1.2.1 Diseas...

Embodiment 2

[0079] Example 2 Cultivation of Canine Distemper Virus Attenuated Vaccine Strain CDV-YBR

[0080] 1 Materials and methods

[0081] 1.1 Test material

[0082] The experimental animals were healthy dogs aged 2 to 4 months (CDV antibody titer <1:2); anti-canine distemper virus specific serum and 1% red blood cell suspension were prepared by our laboratory, and the virulence was the seventh generation of CDV-H (isolated from Example 1).

[0083] 1.2 Cultivation and Identification of Attenuated CDV-YB Strain

[0084] 1.2.1 Cultivation of attenuated strain of CDV-YB

[0085] CDV-YB was passaged through CEF cells to 70 passages, then transferred to Vero cells for another 20 passages, and then returned to CEF cells to passage CDV-YB to 105 passages.

[0086] 1.2.2 Morphological observation of CDV-YB attenuated strain

[0087] Centrifuge the harvested CDV-YB 5, 10, 20, 30, 50, 70, 90, 100, and 105 passage virus cell cultures at 5000r / min for 30min, take the supernatant and ultrace...

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Abstract

The invention discloses a canine distemper attenuated vaccine strain and application thereof. In the invention, passing and cloning are performed on a separated canine distemper virulent strain to culture the canine distemper attenuated vaccine strain, and the microorganism collection number is CGMCC No.3810. The canine distemper attenuated vaccine strain of the invention can provide better protection for the canines suffering homological virulent attack, has perfect immunogenicity and can provide relatively good immune protection for the canines infected with the canine distemper virus. The attenuated vaccine strain can be prepared into single vaccine or united vaccine (live vaccine or inactivated vaccine) and can effectively prevent or cure the canine distemper. The attenuated vaccine strain of the invention has the advantages of stable transmissibility, lasting immunity, good effect, safety, reliability, long storage time and the like.

Description

technical field [0001] The present invention relates to attenuated vaccine strains, in particular to a virulent strain of canine distemper virus CDV-YB and an attenuated vaccine strain attenuated by the virulent strain. The use in distemper biological products belongs to the field of prevention and control of canine distemper. Background technique [0002] Canine distemper is an acute infectious disease of dogs and other animals. Young animals usually have an acute and fatal course, while adult animals can show chronic persistent infection. Clinical symptoms include biphasic fever, mucous membrane catarrh, and central nervous system symptoms. And foot pad swelling is the main feature. The disease has been distributed almost worldwide since it was discovered in 1926. It is currently one of the most harmful diseases to the dog industry, fur animal breeding industry and wildlife protection industry in my country. It often causes a large number of dogs, minks, foxes and other a...

Claims

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Application Information

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IPC IPC(8): C12N7/08A61K39/175A61P31/14C12R1/93
Inventor 刘大飞张洪英曲联东王牟平刘立奎
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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