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Multi-layer porous scaffold and preparation method thereof

A porous scaffold and a technology on a scaffold, applied in the field of multi-layer porous scaffold and its preparation, can solve the problem of lack of connectivity of the scaffold, and achieve the effect of avoiding the residue of harmful substances and avoiding weakened separation

Inactive Publication Date: 2013-07-10
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simply connecting two prefabricated porous scaffolds can easily lead to a lack of connectivity between the scaffolds

Method used

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  • Multi-layer porous scaffold and preparation method thereof
  • Multi-layer porous scaffold and preparation method thereof
  • Multi-layer porous scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Firstly, salt particles and spherical paraffin porogen particles are prepared. The salt particles are crushed mechanically and then sieved through a standard sieve to collect particles in different particle size ranges. Paraffin wax microspheres use 1.0g gelatin and 20.0g paraffin wax, add them into 400ml deionized water, heat to 80°C under 400rpm mechanical stirring, then add 300ml ice water to quench to solidify the wax balls. Filter, wash twice with deionized water, and sieve with standard drying after drying to collect particles in different particle size ranges.

[0045] Next prepare the adhesive. Dissolve 0.5g of PLGA85 / 15 with a molecular weight of 300,000 in 8.0ml of dichloromethane, add 9.5g of sodium chloride salt particles with a particle size of 350-455μm into the polymer solution, mix and stir evenly.

[0046] Take two columnar PLGA85 / 15 porous scaffolds (height 5mm, diameter 10mm, porosity 90%, pore diameter 350-455μm) made of paraffin microspheres, and ...

Embodiment 2

[0048] The support adopts two columnar PLGA85 / 15 porous supports made of paraffin microspheres with different porosities, one of which has a porosity of 95%, and the other has a porosity of 85% (height 5mm, diameter 10mm, pore size 350-455μm), and the remaining conditions and implementation Same as Example 1, a double-layer porous scaffold composed of materials with different porosity was prepared.

Embodiment 3

[0050] The scaffold adopts two columnar PLGA85 / 15 porous scaffolds (height 5mm, diameter 10mm, porosity 90%, pore diameter 350-455μm) obtained by different pore-forming processes, one of which is made of sodium chloride salt particles, and the other is made of paraffin microspheres. Pore-causing, other conditions are the same as in Example 1, and a composite porous scaffold with one layer of regular spherical pores and another layer of irregular pores is prepared.

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Abstract

The invention belongs to the technical field of biological materials and tissue repair and particularly relates to a multi-layer porous scaffold and a preparation method thereof. A suitable porous material is selected, after being clipped according to set size and requirements, the material is bonded by pore-forming adhesive which contains mixture of components of polymer / pore-forming particles / solvent, and pore-forming agent is removed after solidification and bonding, thus preparing the porous scaffold which has multi-layer structures communicated with each other. The scaffold has multi-layer structures, porous transition layers are among the layers of the scaffold, and the pores in an upper layer and a lower layer of the scaffold are communicated with each other; and the scaffold is suitable for transition of different cells and fusion of tissues in a process of tissue remodeling, and weakened separation between layers of the remolding tissue is avoided. The scaffold is suitable for a bionic three-dimensional cell scaffold which repairs a multi-layer tissue and other application fields.

Description

technical field [0001] The invention belongs to the technical field of biological materials and tissue repair, and in particular relates to a multilayer porous support and a preparation method thereof. Background technique [0002] Porous scaffolds can be widely used in tissue repair, cell culture carrier, wound dressing, drug controlled release carrier and other fields. In particular, polymeric porous three-dimensional scaffolds with high porosity have been developed as important components for tissue engineering and in situ tissue regeneration. [0003] The prior art discloses that the basic principle of tissue engineering is: extract certain normal cells from human tissue, culture and proliferate, construct a cell-material complex with a degradable porous scaffold in vitro, and then implant this complex into a tissue defect, With the degradation of materials, the continuous secretion of extracellular matrix and the continuous formation of tissues, new tissues and organs ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/02A61L27/56
Inventor 丁建东张正
Owner FUDAN UNIV
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