Pleuromutilin solvent extraction process

Abstract

The invention relates to an extraction process of microorganism source compounds, in particular to a pleuromutilin solvent extraction process; the process comprises the following steps: mycelium is separated out from pleuromutilin producing bacterium total-fermentation broth, is dried, crushed and sifted out, the total water content of the mycelium is baked to be lower than 5 percent, so as to obtain baking mycelium for spare; the obtained baking mycelium is mixed with butyl acetate, the mixture is shaken out and extracted, the extracting solution is filtered, the filtrate is washed, dehydrated and dried, and decolorized by activated carbon, the solvent is recovered until being crystallized, crystallization is carried out by standing, so as to obtain the pleuromutilin; the process has theadvantages of stability, having easy operation, convenient operation, low solvent toxicity, high safety, low solvent consumption level, short extraction time, easy purification of products and low cost.

Description

technical field [0001] The present invention relates to the extraction process of microbial source compounds, in particular to the solvent extraction process of pleuromutilin. Background technique [0002] Pleuromutilin is a tricyclic diterpenoid broad-spectrum antibiotic, which was first isolated from the basidiomycete Pleurotus mutilis and P.passeckerianus by the scholar Kavanagh et al. in 1951. Basidiomycetes Clitopilus Eleven fungi in the genus can produce such antibiotics. As an intermediate, pleuromutilin has been used in the semi-synthesis of the new veterinary antibiotics Tiamulin and Valnemulin and the new human antibiotic Retapamulin. Among them, retapamulin, which was launched in 2007, is the first brand-new topical antibacterial drug in the world in the past two decades, which marks that the development of Miaolin compounds has achieved a leap from veterinary antibiotics to human antibiotics. Pleuromutilin antibiotics mainly act on the 50S ribosomal subunit of ...

AI Technical Summary

Problems solved by technology

At present, the extraction process adopted by domestic pleuromutilin manufacturers is mainly to separate the mycelium from the whole fermentation liquid by plate and frame filter press method, and then extract it with methanol. This process has the following problems in the actual industrial application process : The extraction time is long; the solvent consumption is large; methanol has a low flash point, is flammable, explosive, volatile, toxic and irritating, and endangers the production environment and the health of operators; methanol has good compatibility with water, and the extract Contains more water-soluble impurities, which are difficult to remove in the later purification process

In recent years, foreign patents have also reported the use of methyl isobutyl ketone to extract and purify the whole fermentation broth. Although this process simplifies the operation, the solvent consumption is relatively large and the cost is relatively high.

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