Universal strong promoter among species derived from white spot syndrome virus and application thereof

Abstract

The invention relates to a universal strong promoter among species derived from white spot syndrome virus and an application thereof. The invention provides the universal strong promoter among the species derived from the white spot syndrome virus, two primers of the strong promoter, an expression vector of the strong promoter and recombinant host cells of the expression vector. The promoter is an immediate early gene promoter of the white spot syndrome virus and can high-efficiently start the expression of downstream genes in insect cells, mammalian cells and the white spot syndrome virus host cells (including cells of crayfish and shrimp). The recombinant host cells are obtained by transfection of the expression vector containing the universal strong promoter among the species derived from the white spot syndrome virus in the host cells, and the recombinant host cells can express target protein genes controlled by the universal strong promoter among the species derived from the white spot syndrome virus.

Description

technical field [0001] The present invention relates to a promoter, in particular to a strong interspecies general promoter derived from white spot syndrome virus, and an expression vector containing the above promoter, which is used for expressing foreign genes in cells of different species. Background technique [0002] In the field of genetic engineering, the recombinant expression of target genes is of great significance both in theoretical research and practical application. To express the target gene in the host cell, it must be cloned into a genetic engineering vector with various elements required for gene expression, which is called an expression vector. Gene expression systems can be divided into prokaryotic expression systems and eukaryotic expression systems. [0003] Escherichia coli is currently the most widely used prokaryotic expression system, which can express and purify recombinant proteins conveniently and efficiently. However, when the target gene is a...

AI Technical Summary

Problems solved by technology

[0006] Although there are powerful promoters in several expression systems commonly used at present, which can ensure the high-efficiency expression of genes, but because the promoters carried by the vectors are species-specific, these expression vectors are universal among different expression systems Not strong, so when it is necessary to express foreign genes in different species, people need to replace the appropriate expression vector for the target gene through experimental steps such as gene amplification, enzyme digestion, and ligation, which is time-consuming and laborious

Although some companies have developed some methods based on DNA recombination technology (such as Invitrogen's Gateway system) in recent years to facilitate the transfer of target genes between different expression vectors, the application of this method still requires the replacement of vectors, and the experimental cost is much higher Compared with the traditional gene cloning method (such as Invitrogen's Gateway system, the price of the enzyme used in a cloning experiment needs to be 100-200 yuan, while the enzyme used for a cloning in the traditional method is generally less than 10 yuan), so the application of this new method does not Not widely, and constructing a vector that can be used in different expression systems will be an effective way to solve this problem

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