Method for preparing hogcholera vaccine

Abstract

The invention discloses a method by adopting cell micro-carrier suspension culture system to prepare hogcholera (CSF) vaccine, which comprises the following steps that: (1) cells for preparing the vaccine is inoculated into a carrier tank containing culture liquid and micro-carriers, and the cells are uniformly mixed with the micro-carriers, so the cells are attached onto the micro-carriers; (2) when the quantity of the cells is increased to 5 to 40 times of the initial inoculation concentration, the hog cholera virus (HCLV) is inoculated onto the cells so as to breed the virus according to the virus multiple of infection (M.O.I) is of the ratio of 0.01 to 1; and (3) the prepared virus liquid is mixed, appropriate freezing drying protective agent is added, the virus liquid is quantitatively packed after being uniformly mixed with the freezing drying protective agent to be frozen and dried so as to obtain the hogcholera vaccine (CSF). The method which is adopted to produce the hogcholera vccine has the advantages that the concentration of the cultured cells is high, the cells can be continuously cultured, the virus yield is high, the immunity effect of the vaccine is high, the safety is good, and the like, and has complete immunity protection effect against the attack of the hog cholera virus.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a method for preparing a swine fever vaccine. Background technique [0002] Classical Swine fever (CSF for short), also known as Hog cholera or European swine fever, is a viral infectious disease with acute or hyperacute fever in pigs, clinically characterized by Rapid dissemination, fever, and typical hemorrhagic lesions can be seen on autopsy. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. This disease is one of the most important infectious diseases threatening the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 notifiable infectious diseases of category A, and China defines it as a severe infectious disease. [0003] Compulsory immunization with swine fever live virus vaccine is currently the...

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Problems solved by technology

[0005] Among them, the tissue seedling method and the primary bovine testicular cell seedling method generally have shortcomings such as low yield, low efficiency, foreign virus contamination, low toxin production titer, and large batch-to-batch differences; the cell line method, although it has solved the problem of exogenous virus Pollution problem, the titer of toxin production has also been greatly improved, and the difference between batches has also been reduced, but the vaccine production still cannot be greatly improved, and the process is cumbersome and inefficient, resulting in a lot of waste of personnel

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