Method for preparing tissue engineering cornea

Abstract

The present invention discloses a producing method for tissue engineering cornea. The method involves using amniotic epithelial cells and amniotic stromal cells as seed cells, planting at two sides of decellularized natural corneal stroma after inducement and differentiation in vitro, forming tissue engineering cornea through organ culture in vitro. The scaffold of present invention not only resolves the problem of strong immunological rejection of heterogenetic corneal stroma, but also sustains the structure (which can restore transparency of cornea) and major components (including growth factors which could promote corneal cell growth, proliferation and differentiation) of natural cornea. Compared with the prior art, present invention has advantages of low cost, easy operation, wide range of sources and easy preservation. The said tissue engineering cornea containing live cells has certain elasticity and tenacity, and its shape, size and thickness can be changed easily. The tissue engineering cornea has very low immunogenicity. It could avoid complications caused by non-corneal material, be reconstructed by recipient cells after being transplanted in body, and be incorporated into organism rapidly to become transparent. It could be used to repair corneal wounds caused by all kinds of reasons.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a preparation method of tissue engineering cornea. Background technique [0002] The anatomical characteristics of the eyeball determine that the position of the eyeball is exposed, the tissue structure is fragile, and a small external force or foreign body can cause serious damage. In addition, some corneal diseases include infectious keratopathy, corneal degeneration, malnutrition and immune keratopathy, etc. Can cause corneal damage. Once damaged, it will seriously affect vision or cause blindness. Corneal lesions are the second leading cause of blindness next to cataracts, and the number of cases is increasing at a rate of 1.5 million to 2 million cases per year. While keratoplasty is the most effective means of treating corneal lesions, donors for traditional keratoplasty mainly come from cadavers and donations. At present, t...

AI Technical Summary

Problems solved by technology

[0004] The current tissue-engineered cornea mainly uses amniotic membrane with decellularized components as a carrier, and limbal stem cells are inoculated on the surface to construct it. The main disadvantage is that the source of limbal stem cells is limited, and the amniotic membrane depleted of cellular components cannot play the role of amniotic membrane stem cells; at the same time The amniotic membrane structure is very thin, has no strength, and cannot repair corneal defects, making it ineffective in clinical application