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Drug compound for preventing and curing diabetes mellitus

A composition and a technology for diabetes are applied in the field of compositions extracted from natural plants, and can solve the problems of complex process route, high production cost, lack of significant medicinal effect, effective ingredients and mechanism of action, etc.

Active Publication Date: 2010-06-23
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although various existing Chinese patent medicines have a certain therapeutic effect on diabetes, the single-component preparations currently used clinically or the prescription decoctions prepared by doctors lack significant efficacy, clear active ingredients and mechanism of action, and the quality is controllable and reliable. Modern traditional Chinese medicine with convenient dosage form
Chinese invention patent ZL200510110763.2 discloses a composition for treating diabetes, which is composed of Anemarrhena anemarrhena, Cortex Phellodendri and cinnamon extracts. The route is more complicated

Method used

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  • Drug compound for preventing and curing diabetes mellitus
  • Drug compound for preventing and curing diabetes mellitus
  • Drug compound for preventing and curing diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Determination of timosaponin A-III, B-II and B-III content

[0089] Column: Diamonsil TM C 18 (200mm×4.6mm, 5μm). Column temperature: 25°C, flow rate 1.0ml / min. ELSD detector, drift tube temperature 110°C, nebulizing gas flow rate 2.6 SPLM. The injection volume was 20 μl.

[0090] Mobile phase A: acetonitrile; mobile phase B: 0.5% aqueous formic acid; equilibrated with 30% mobile phase B, eluted with a linear gradient over 30 minutes, and terminated with 90% mobile phase B.

[0091] Accurately weigh 25.0 mg each of Timosaponin A-III, B-II and B-III standard products into a 10 ml measuring flask, add appropriate amount of methanol, ultrasonically treat to dissolve, let cool, add methanol to dilute to the mark, shake well, Reconfigured into 1.0mg / ml Timosaponin B-II, B-III reference solution and 1.0mg / ml Timosaponin A-III reference solution for later use.

[0092] Accurately weigh 25mg of total Anemarrhena saponin extract, put it in a 25ml volumetric flas...

Embodiment 2

[0096] Example 2 Determination of mangiferin content

[0097] Column: Diamonsil from Dima R C 18 Column (250 mm x 4.6 mm, 5 μm); column temperature was 20°C.

[0098] Mobile phase: acetonitrile-water (15:85, v / v), (pH adjusted to 3.0 with phosphoric acid); flow rate: 1 ml / min; injection volume 20 μL.

[0099] Detection wavelength: 254nm.

[0100] Precisely weigh 6.3 mg of mangiferin reference substance, put it in a 25ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat it to dissolve, let it cool, add methanol to dilute to the mark, shake well, and prepare a 0.252mg / ml mangiferin reference substance solution for use. .

[0101] Accurately weigh 10 mg of Anemarrhena chinensis total flavonoids extract, put it in a 25ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat it to dissolve, let it cool, add methanol to dilute to the mark, shake well, and then dilute to an appropriate concentration, it is the total flavonoids o...

Embodiment 3

[0105] Example 3 Determination of Berberine Content

[0106] High performance liquid chromatography: Agilent 1100 system; Hangzhou Yingpu chromatography workstation.

[0107] Column: Diamonsil TM C 18 (250mm×4.6mm, 5μm); the column temperature is 20°C; the injection volume is 20μl.

[0108] Mobile phase: water-acetonitrile-glacial acetic acid-triethylamine (67:33:0.3:0.8, v / v), detection wavelength: 345 nm; flow rate: 1 ml / min.

[0109] Accurately weigh 10.0 mg of berberine hydrochloride into a 25 ml volumetric flask, add methanol to dissolve to the mark, prepare a 400 μg / ml stock solution, accurately draw 0.5 ml of the berberine stock solution, add methanol to dissolve and dilute to 10 ml, Make 1ml of reference solution containing 20μg of berberine.

[0110] Precisely weigh 10 mg of Phellodendron chinensis extract, put it in a 25ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat it to dissolve, let it cool, add methanol to dilute to the mark,...

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PUM

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Abstract

The invention discloses a drug compound for preventing and curing diabetes mellitus, which at least comprises two components including Timosaponin B-II and berberine. Preventing and curing effect can be improved by adding one or a plurality of components from mangiferin, Timosaponin A-III and Timosaponin B-III. The drug compound can also be compounded by anemarrhena extractive and golden cypress extractive, or can be directly extracted from anemarrhena and golden cypress. The drug compound has the advantages of obvious blood sugar reduction effect, and high effective component content, easy quality control. The preparation process of the drug compound is easy for industrialization, and can be applied as drugs to prevent and cure diabetes mellitus.

Description

technical field [0001] The present invention relates to a composition extracted from natural plants, in particular to a composition extracted from Anemarrhena and Phellodendron, for the prevention and treatment of diabetes. Background technique [0002] Diabetes Mellitus (DM) is a series of clinical syndromes caused by absolute or relative insufficiency of insulin in the body. The disease will also cause some complications in the development process, such as: hypoglycemia (Hypoglycemia), ketoacidosis (Ketoacidosis), nonketotic hyperosmolar coma (Nonketotic Hyperosmolar Coma), cardiovascular disease, chronic renal failure, Retinopathy, neuropathy and microvascular disease. There are three main types of drugs commonly used in the treatment of diabetes: biological drugs, such as insulin (Insulin); chemical drugs, such as: sulfonylurea (Sulfonylurea), biguanide (Biguanide), glitazone (Glitazone) and Chinese patent medicine kind. Due to the lack of a relatively mature non-inje...

Claims

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Application Information

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IPC IPC(8): A61K36/8964A61K31/7048A61P3/10A61K31/4375A61K31/352
Inventor 黄成钢范明松唐意红李志雄孙兆林吴斌马春晖刘会会
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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